No abstract available.
Colonic Pouches* ; Cystectomy* ; Urinary Bladder Neoplasms* ; Urinary Bladder*

No abstract available.
Urinary Bladder Neoplasms* ; Urinary Bladder*

No abstract available.
Bacillus* ; Mitomycin* ; Urinary Bladder Neoplasms* ; Urinary Bladder*

No abstract available.
Carcinoma, Renal Cell* ; Cell Line* ; Humans*

OBJECTIVE: To evaluate the effect of transduced tumor necrosis factor-a(TNF-a) gene expression on growth of human bladder tumor cell lines in vitro. MATERIALS AND METHODS: The complete cDNA of TNF-a was introduced to three human bladder tumor cell lines(F-24, J-82, HT-1197) using a retroviral vector, a recombinant form of Molony murine leukemia virus with TNF-a and Neo gene and transfected cells were selected by exposure to neomycin analog G418. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR)-Southern blotting. Cell growth was measured by MTT assay Result is Successful gene transfer and expression were confirmed in all three cell bladder tumor lines. Growth of transfected cells were compared with parental cell lines and no differences were found in all three cell lines(p>0.05). CONCLUSION: Expression of transduced TNF-t gene could not show any effect on growth of human bladder tumor cells in vitro.
Cell Line* ; DNA, Complementary ; Gene Expression* ; Genetic Therapy ; Humans* ; Leukemia Virus, Murine ; Necrosis ; Neomycin ; Parents ; Reverse Transcription ; Tumor Necrosis Factor-alpha* ; Urinary Bladder Neoplasms* ; Urinary Bladder* ; Zidovudine

OBJECTIVE: To evaluate the effect of tumor necrosis factor-alpha (TNF-alpha)gene transduced bladder tumor cells on survival of C3H/He mice. MATERIALS AND METHODS: The complete cDNA of TNF-alpha was introduced to mouse bladder tumor cell line(MBT-2) using a retroviral vector. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR). Produced TNF-alpha was measured by ELISA. TNF-alpha gene transduced and parental cells were xenografted in C3H/He mice. Implantation and growth rate and survival of experimental animals were analysed. RESULTS: Successful gene transfer and expression were confirmed. TNF-G gene transduced MBT-2 cells secreted TNF-alpha at the concentration of 47.4pg/106cells/24hours. The implantation and growth rate of TNF-t gene transduced MBT-2 cells were much lower than those of parental cells and survival of experimental animals was different between TNF-t gene transduced MBT-2 and parental cells(P<0.05). CONCLUSION: This study shows potential advantages of localized TNF-a secretion to induce potent antitumorigenic response. Tumor cell targeted TNF-a gene therapy could become a useful tool in treatment of bladder cancer.
Animals ; DNA, Complementary ; Enzyme-Linked Immunosorbent Assay ; Genetic Therapy ; Heterografts* ; Humans ; Mice* ; Parents ; Reverse Transcription ; Tumor Necrosis Factor-alpha* ; Urinary Bladder Neoplasms* ; Urinary Bladder* ; Zidovudine

We analyzed 57 patients with tuberculous nonfunctioning kidneys, which were diagnosed pathologically or bacteriologically at Seoul National University, from October 1981 to December 1989. The patients were divided into 3 groups according to the treatment ; Group 1 : 12 patients, only chemotherapy for more than 12 months ; Group 2 :32, nephrectomy and chemotherapy for more than 6 months ; Group 3 . 13, nephrectomy and chemotherapy only for 3 months. As a standard antituberculous regimen, isoniazid, rifampin, and ethambutol were used, and pyrazinamide was added in 15 patients. The follow-up tests were urinalysis, urine AFB smear and culture, and intravenous pyelography. The duration of the follow-up was 6 to 1O8 months, with a mean of 23.3 months. There was no evidence of recurrence in any patient among the 3 groups. Two postoperative complications (hematoma and fistula formation) occurred in 45 nephrectomized patients. Pathologic examination of the resected kidneys revealed findings compatible with tuberculosis in all patients of Groups 2 and 3. Coexisting cortical adenomas were found incidentally in 2 patients. Final diagnoses of the other 2 patients were hydronephrosis and chronic pyelonephritis without any evidence of tuberculosis, although these 2 patients were excluded in this study. Severe drug toxicity occurred in 7 patients that belonged to Groups 1 and 2. In conclusion, early nephrectomy for accurate diagnosis and removal of infective foci was the justifiable initial procedure, and this should be followed by postoperative antituberculous chemotherapy for the shortest acceptable period in tuberculous nonfunctioning kidneys. In highly selective cases where the lesion is completely localized in one kidney, we can consider early nephrectomy and a 3-month course of chemotherapy as one treatment modality.
Adenoma ; Diagnosis ; Drug Therapy ; Drug-Related Side Effects and Adverse Reactions ; Ethambutol ; Fistula ; Follow-Up Studies ; Humans ; Hydronephrosis ; Isoniazid ; Kidney* ; Nephrectomy ; Postoperative Complications ; Pyelonephritis ; Pyrazinamide ; Recurrence ; Rifampin ; Seoul ; Tuberculosis ; Urinalysis ; Urography

No abstract available.
Urinary Tract*

No abstract available.
Carcinoma, Renal Cell* ; Prognosis*

Adenocarcinoma of the bladder is rare. During the last 20 years 13 patients with adenocarcinoma of the bladder were managed at Seoul National University Hospital. The incidence is 2.2% of all primary epithelial bladder neoplams. 10 patients had primary adenocarcinoma and urachal origins were identified in 3 patients. The most common presentation was painless gross hematuria. 9 of 10 patients with primary adenocarcinoma had deeply infiltrating tumor with or without distant metastasis at the time of initial diagnosis. Various interventions including TUR and partial cystectomy were carried out. In 5 patients postoperative radiation therapy was tried without substantial improvement. Three year survival rate of primary adenocarcinoma had was averaged less than 25% regardless of the mode of treatment. One patient was free of cancer up to 3 years. 3 patients with urachal adenocarcinoma had stage D of mucin producing adenocarcinoma. Partial cystectomy including enbloc excision of urachus in 1 was carried out, partial cystectomy in 1 and exploration for unresectable tumor in one patient who died in one year.
Adenocarcinoma* ; Cystectomy ; Diagnosis ; Hematuria ; Humans ; Incidence ; Mucins ; Neoplasm Metastasis ; Seoul ; Survival Rate ; Urachus ; Urinary Bladder Neoplasms ; Urinary Bladder*