OBJECTIVE: To evaluate the therapeutic effect of Shugan huoxue huatan recipe for patients with non-alcoholic steatohepatitis (NASH). METHDOS: 72 NASH patients (treating group) treated with Shugan huoxue huatan recipe one unit dose daily (in two divided doses) for 3 months, and 36 cases (control group) with Hugan (liver-protection) tablets (4 tablets/ time tid) and Febuprol tablets (100 mg/time tid) for 3 months. The B-ultrasonic examination, clinical symptoms, physical sign, liver function and blood lipid were observed before and after the treatment. RESULTS: The recipe could significantly improve the ultrasonic image, relieve clinical symptoms and physical signs, improve liver function, and lower the body weight, BMI and serum contents of triglyceride and cholesterol in NASH patients. Its total effect rate was 79.17%, higher than 59.36% of control group (P

Objective To understand the status quo of related knowledge,attitude and practice of religious belief among undergraduate nursing students and provide reference for education on religious belief.Methods A self-filled questionnaire on religious belief was used randomly among undergraduate nursing students in 4 medical universities in Wuhan.Results Most nursing students were inferior to the cognitive degree of related knowledge on belief religious,which was greatly related to the grade,nationality,parents’ religious belief and family residence factors.The notion of nursing students towards religious belief policy was nebulous.Most students could cognize the relationship between religion and science correctly.The university had minor effect on students’ religion view.Students contacted religious belief with various channels and the role of the network could not be underestimated.Undergraduate nursing students understood and pardoned their religious belief and other students’.Their religious belief was not the mainstream of current belief.The Buddhist belief of religion was in the first place.The majority of students went to religious activities not for the belief.Conclusions We should face the reality,examine rationally at this society and strengthen the social,school,family and individual guidance on religion together with related education coustruction.

OBJECTIVETo observe the effects of autologous blood injection and 0.9% NaCl at Zusanli (ST 36) on ovarian function in patients with primary ovarian insufficiency.

METHODSSixty patients with primary ovarian insufficiency were randomly divided into an observation group and a control group, 30 cases in each one. The patients in the observation group were treated with injection of autologous blood at Zusanli (ST 36); the patients in the control group were treated with 0.9% NaCl with identical volume at Zusanli (ST 36). Both the treatments were given once a week for 3 months. The ovarian function, including follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E) were tested before treatment, 1 month, 2 months and 3 months after first acupoint injection; the endometrial thickness before and after treatment and clinical efficacy were compared in the two groups.

RESULTSCompared before treatment, FSH was lowered in the observation group after 1-month treatment (<0.05), while FSH and LH were lowered and Ewas increased after 2-month treatment and 3-month treatment (all<0.05). Compared with 1-month treatment, FSH and LH were lowered and Ewas increased in the observation group after 2-month treatment and 3-month treatment (all<0.05). Compared with 2-month treatment, FSH was lowered and Ewas increased in the observation group after 3-month treatment (both<0.05). The differences of all serum tests before and after treatment were insignificant in the control group (all>0.05). The FSH after 1-month treatment, and FSH, LH and Eafter 2-month treatment and 3-month treatment in the observation group were significantly different from those in the control group (all<0.05). The endometrial thickness after treatment in the observation group was higher than that before treatment (<0.05), while the endometrial thickness after treatment in the control group was similar to that before treatment (>0.05); the difference of endometrial thickness before and after treatment in the observation group was higher than that in the control group (<0.05). The clinical effective rate was 83.3% (25/30) in the observation group, which was superior to 46.7% (14/30) in the control group (<0.05).

CONCLUSIONThe autologous blood injection at Zusanli (ST 36) can significantly improve ovarian function, promote endometrial growth in patients with primary ovarian insufficiency.

Objective To observe the effect of electroacupuncture at Hegu (LI04) on capillary density and expression of CD34 after focal cerebral ischemia/reperfusion in rats. Methods 90 rats were randomly divided into normal group (n=6), model group (n=42) and electroacupuncture group (n=42). A rat model of focal cerebral ischemia/reperfusion was made by filament occlusion in the model group and the electroacupuncture group. And the electroacupuncture group received electroacupuncture. They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after reperfusion 1 h followed ischemia. HE staining was used to observe and calculate the infarct volume and capillary density, and immunohistology was used to detect the expression of CD34 after neurologic symptoms rating. Results Compared with the model group, the neurologic symptoms score significantly decreased 2 h after reperfusion in the electroacupuncture group (P<0.01), and still decreased 21 d after reperfusion (P<0.05). The infarct volume significantly was smaller in the electroacupuncture group than in the model group (P<0.01). The capillary density was higher 2 h, 24 h, 3 d, 7 d, 14 d after reperfusion in the electroacupuncture group than in the model group (P<0.05). Compared with the model group, the expression of CD34 significantly increased 3 d after reperfusion in the electroacupuncture group (P<0.01),and it reached a peak at 7 d after reperfusion (P<0.01), and still higher at 14 d. Conclusion Electroacupuncture can promote the expression of CD34 and the capillary density, and stimulate the angiogenesis after focal ischemia/reperfusion.

【Objective】 To explore the characteristics and prevalence of HBV infection among voluntary blood donors in Chongqing and analyze its epidemiological characteristics, so as to provide reference for effective selection of low-risk blood donors. 【Methods】 The test results of hepatitis B surface antigen (HBsAg) and viral nucleic acid (HBV DNA) of 314 442 voluntary blood donors from October 2017 to October 2020 in Chongqing Blood Center were retrospectively analyzed. The relationship between the total positive rate of HBV, HBsAg+ /HBV DNA-, HBsAg-/HBV DNA+, HBsAg+ /HBV DNA+ and gender, marriage, age, education background, occupation, firs-time blood donation and repeated blood donation was analyzed. 【Results】 Among the 314 442 voluntary blood donors, the total positive rate of HBV, HBsAg+ /HBV DNA-, HBsAg-/HBV DNA+ and HBsAg+ /HBV DNA+ was 0.88% (2 764/314 442), 0.28% (893/314 442), 0.18% (567/314 442) and 0.41% (1 304/314 442), respectively. The total positive rate of HBV, HBsAg-/HBV DNA+ and HBsAg+ /HBV DNA+ was different in gender, marriage, age, education background, occupation and firs-time/repeated blood donation. The positive rate of men was higher than that of women, and that of married higher than that of unmarried, and decreased as the education level elevated.The positive rate of farmers and workers was the highest and that of medical staff was the lowest, followed by students, military personnel, civil servants, and teachers. The positive rate of first-time blood donors was higher than that of repeated blood donors. The differences were statistically significant (P<0.05); The positive rate of HBV, HBsAg-/HBV DNA+ and HBsAg+ /HBV DNA+ within 18~50 years old all increased gradually with age. the positive rate of HBsAg+ / HBV DNA+ was highest within 41~50 years old, and that of HBsAg-/HBV DNA+ was highest within 51~60 years old, with statistical significance (P<0.05). The positive rate of HBsAg+ /HBV DNA- was statistically significant between first-time and repeated blood donation (P<0.05), and there is no significant difference between gender, marriage, age, educational background, and occupation(P>0.05). 【Conclusion】 The prevalence of HBV among voluntary blood donors in Chongqing is relatively high and is related to gender, marriage, age, education background, occupation, and first-time/repeated blood donation. It is necessary to strengthen effective consultation before blood donation, formulate guidelines for blood donation recruitment, select voluntary blood donors from low-risk groups to improve blood safety.

[Objective] To assess the data characteristics of quality monitoring indicators for red blood cell (RBC) preparations, so as to provide reference for continuous improvement of blood quality. [Methods] The quality inspection data of 6 types of RBC preparations from Chongqing blood center from 2019 to 2023 were summarized. For the same indicators, the numerical range of quality indicators was monitored by comparing different types of preparations with the national standard GB18469. The loss and/or damage to RBCs caused by different preparation process were compared, and the impact of different preparation processes on the quality of RBCs was discussed. [Results] The appearance and sterility test compliance rates of the six types of RBC preparations were both 100%, while the compliance rates of other items were all ≥75%. The compliance rate of hematocrit for suspended RBCs was the lowest at 75%, with a median of 0.52, which was close to the lower limit of GB18469, while the medians of hematocrit for the other types were all at the midline level of GB18469. The Hb content for different types of RBCs was significantly higher than the corresponding requirements of GB18469 (P<0.05). The hemolysis rate at the end of storage for different types of RBCs was significantly lower than the requirements of GB18469 (P<0.05). The 1 U leukoreduction process resulted in a hemoglobin content loss of about 5% and had a significant impact on the hemolysis rate at the end of storage (P<0.05). The washing process resulted in a hemoglobin content loss of <3% and had no significant impact on the hemolysis rate at the end of storage (P>0.05). The concentration process resulted in a hemoglobin content loss of <3% and had a significant impact on the hemolysis rate at the end of storage (P<0.05). [Conclusion] The impact of different processes on RBC preparations is within a controllable range and meets the requirements of GB18469. The quality monitoring data can provide a reference for clinical blood selection, effectiveness evaluation and revision of related standards.

【Objective】 To provide reference for formulating relatively unified quality control strategies and meeting the requirements of homogenization construction of blood banks across Chongqing area by retrospectively analyzing sampling results of different blood components during the past two years in all levels of blood banks in Chongqing area. 【Methods】 The key quality data of blood components prepared by 6 blood banks in Chongqing were analyzed retrospectively. According to the issuing units to the clinical during the past two years, the research objects were selected as leukocyte-depleted suspended RBCs, cryoprecipitate, pathogen inactivated fresh frozen plasma(FFP) and apheresis platelets. The quality data of the above-mentioned blood components from January 2019 to June 2021 were collected and analyzed. 【Results】 For leukocyte-depleted suspended RBCs(1U)prepared by 5 blood banks, statistically significant differences in Hb, residual white blood cells and hemolysis rate at the end of storage, except for Hct, were noticed(P<0.05). For cryoprecipitate, the content of blood coagulation factor Ⅷ and fibrinogen were statistically different among 3 blood banks in 1U specification(P<0.05) and among 5 blood banks in 2U specification(P<0.05). For pathogen inactivated FFP, the content of blood coagulation factor Ⅷ, plasma proteins, and residual methylene blue were statistically different among 3 blood banks(P<0.05). For apheresis platelets, Plt, white/red blood cells contamination and pH at the end of storage were statistically different among 3 blood banks(P<0.05). 【Conclusion】 The quality data of blood components, prepared by different blood banks, meet the requirements of national standard, however, certain differences are existing among blood banks.Key points during the process of collection, preparation, storage and transportation need to be cleared and unified, so as to reduce the differences between each other, and determine the direction and basis for homogeneity construction in the next step.

【Objective】 To identify a case of antibody against highly prevalent antigen through molecular biology technology. 【Methods】 Blood group typing, unexpected antibody identification and cross matching were performed by serological test, and genetic testing of Diego blood group was performed by molecular biology technology. 【Results】 Serological test showed that there was a high prevalence of anti-Di^b in the serum of the patient. Gene sequencing showed that the genotype of the patient was Di(a+b-) . Two cases with Di(a+b-) matched with the patient were screened from 856 blood donors. 【Conclusion】 The combined detection method based on serological test supplemented by molecular biology technology is beneficial to the detection of antibody against highly prevalent antigens, and is of great significance for ensuring the safety of clinical blood transfusion.

[Objective] To analyse the distribution of antigen phenotypes in the Rh, MNS and Kidd blood group systems of Han and Tujia blood donors in Chongqing, and to provide data support for the establishment of an expanded blood group antigen phenotype database and the development of expanded blood group coordinated transfusion in blood donors. [Methods] The antigens of Rh, MNS and Kidd blood group systems in Han and Tujia blood donors in Chongqing were detected by test-tube method, and the Hardy-Weinborg anastomosis of the three blood group systems was calculated. Pearson's chi-square test and Fisher's exact probability method were used to compare the differences in phenotypic distribution frequencies among different regions and ethnic groups. [Results] Han and Tujia blood donors accounted for the highest proportion of CCee in the antigenic phenotype of the Rh blood group system, followed by CcEe, and then Ccee and ccEE. Tujia blood donors accounted for 52.02% of CCee, which was higher than that of Han blood donors (47.24%), while Han blood donors accounted for 32.20% of CcEe, which was higher than that of Tujia blood donors (28.94%). In the antigenic phenotype of the MNS blood group system, the blood donors of Han nationality and Tujia were MN>MM>NN,. The antigen phenotype distribution frequency of the Kidd blood group system was highest for Jk(a+b+) among both Han and Tujia blood donors, and the blood donors of Han nationality were Jk(a+b+)>Jk(a+b+), while those of Tujia were Jk(a-b+)>Jk(a+b-). The antigens of the three blood groups of Han and Tujia blood donors were consistent with the Hardy-Weinberg balance(P>0.05). There was no statistically significant difference in the frequency of antigen phenotypes of the three blood group systems between Han and Tujia blood donors(P>0.05). There were statistically significant differences in the phenotypic distribution frequency of Rh antigens between Chongqing and Xi'an, Zhejiang, Shantou, Foshan, Nanning and Yangzhou(P<0.05), but not with Guang'an and Shenzhen(P>0.05). There were statistically significant differences in the phenotypic distribution frequency of Rh antigens between Han, Tujia, Zang, Mongolian, Korean and Hani ethnic groups in Chongqing(P<0.05). There were statistically significant differences in the phenotypic distribution frequency of MNS antigens between Han blood donors in Chongqing and Urumqi, Hainan and Yuncheng, but not with Xi'an and Wenzhou. There was a statistically significant difference in the phenotypic distribution frequency of MNS antigen between Tujia blood donors in Chongqing and Urumqi and Hainan(P<0.05), but there was no significant difference in the phenotypic distribution frequency of MNS antigen between Tujia blood donors in Chongqing, Urumqi and Hainan(P>0.05). There was a statistically significant difference in the phenotypic distribution frequency of Kidd antigens between blood donors in Chongqing and Harbin(P<0.05), but not in Huizhou, Wenzhou and Yichang(P>0.05). [Conclusion] The population in Chongqing has multi-ethnic characteristics, and the antigenic phenotypes of Rh, MNS and Kidd blood group systems exhibit diversity and regional differences. Establishing an expanded blood bank can provide more options for precision blood transfusion.

Paper-based microfluidic, as a rapid, low-cost and portable detection technology, has been widely used in many fields such as disease diagnosis, food safety and environmental monitoring, especially in laboratory medicine. Compared with conventional methods, paper-based microfluidic provides reliable support for rapid and immediate diagnosis of diseases due to its unique physical and chemical properties of test strips. Based on the construction scheme and result analysis strategy of paper-based microfluidic, this paper reviews its application in clinical detection of nucleic acid and protein, and looks forward to its potential opportunities and challenges in laboratory medicine.