Objective To understand the status quo of related knowledge,attitude and practice of religious belief among undergraduate nursing students and provide reference for education on religious belief.Methods A self-filled questionnaire on religious belief was used randomly among undergraduate nursing students in 4 medical universities in Wuhan.Results Most nursing students were inferior to the cognitive degree of related knowledge on belief religious,which was greatly related to the grade,nationality,parents’ religious belief and family residence factors.The notion of nursing students towards religious belief policy was nebulous.Most students could cognize the relationship between religion and science correctly.The university had minor effect on students’ religion view.Students contacted religious belief with various channels and the role of the network could not be underestimated.Undergraduate nursing students understood and pardoned their religious belief and other students’.Their religious belief was not the mainstream of current belief.The Buddhist belief of religion was in the first place.The majority of students went to religious activities not for the belief.Conclusions We should face the reality,examine rationally at this society and strengthen the social,school,family and individual guidance on religion together with related education coustruction.

The article describes the current situation of training mode of community nurses in community health service institutions in Hubei province from several aspects,including training object,training target,training base,teaching staff,training organization and government,training method and time,training curriculum,training effect evaluation and certification.The article suggests a multitude of factors influencing the training quality,such as formal training,dominant job training,and monotonous community training content and less training time.Overall,the government should be done something to increase government support and funding and accelerate the community nursing services and community nursing education.It is also essential to establish community nursing personnel training mechanism,improve and perfect the community health personnel training system,increase the construction of community nursing practice base and facilities and strengthen clinical practice skills.

To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank. Then we detected the changes in the expression of Pr1 gene before and after infecting H. gonggaensis using real-time quantitative PCR. We obtained the partial sequence of Pr1 gene with the length of 535 bp (GenBank accession: KC009680). Real-time PCR results showed that the expression level of Pr1 gene was significantly different among 8 samples (P < 0.01). Pr1 gene showed the obvious higher expression level (2-3 folds) after infecting the H. gonggaensis, suggesting that the Pr1 gene may play an important role in the process of H. sinensis infecting H. gonggaensis. The present study paves a way for further identification on infectivity assessment of H. sinensis.
Amino Acid Sequence ; Animals ; Hypocreales ; genetics ; metabolism ; pathogenicity ; Larva ; Lepidoptera ; microbiology ; Real-Time Polymerase Chain Reaction ; Subtilisin ; genetics ; metabolism

【Objective】 To investigate the management of adverse reactions to blood donation(ARBD) in blood services, so as to promote the surveillance of ARBD and improve the quality of blood donation service in Chongqing. 【Methods】 A questionnaire, involving the staff and facilities in blood donation sites as well as the prevention and treatment, the record and report, the following up and data related to ARBD was developed by Chongqing Society of Blood Transfusion in February 2019, and was issued to 18 blood services(1 blood center and its sub-center, 6 central blood stations and 11 hospital blood banks) in the Chongqing via email. The questionnaire was filled in and submitted before March 31 by management personnel participating in the investigation, and the data was collected, collated, revised and analyzed by Excel 2011. 【Results】 A total 19 questionnaires were collected, with the valid rate at 100%(19/19). 78.95%(15/19) of the blood services met the requirements of medical personnel allocation(>6 medical staff) when the number of daily blood collection was more than 60, and 100%(19/19)met the requirements of medical personnel allocation(2 to 6 medical staff) when the number of daily blood collection was less than 60. 89.47%(17/19) of the blood services were equipped with epinephrine hydrochloride, and 84.21%(16/19) with dexamethasone(an anti-allergic drug). There were significant differences in the allocation of other types of drugs. 100.00%(19/19) of the blood services formulated prevention and treatment measures concerning ARBD. In 2019, the incidence of ARBD in Chongqing was reported to be 0.54%(1 958 / 359 871), with the highestas [1.35%(223/16 543)] in subcenters and the lowest [0.32%(179/56 299)] in central blood centers (P<0.05). There was statistical significances in the incidences of ARBD reported by different blood stations(P<0.05). 【Conclusion】 The monitoring and management of ARBD among blood services in Chongqing should be further standardized in terms of staffing allocation, emergency drugs allocation and reporting, so as to gradually realize regional homogenization and ensure blood safety.

With retrieval of the ancient medical classics, the descriptions on meridians and collaterals are checked, and the different methods, way of thinking and presuppositions on experiment of propagated sensation along meridian are studied and compared in this paper. The result shows that the basic feature of meridians and collaterals is based on not the propagated sensation along meridian, but the arteriopalmus. Therefore, propagated sensation along meridian can not reflect the entity of meridian. It is the abuse of inference to conclude that the result of "experiment of progated sensation along meridian" equals to meridians and collaterals. The experimental methods are difficult to be applied on part of the meridian theory that is gained through experiences and practice. Thus, a new foothold should be taken as the basis of study on essence of meridians.
Acupuncture Points ; Humans ; Meridians ; Sensation

Postherpetic neuralgia (PHN) is the most troublesome side effect of Herpes Zoster (HZ), which mainly affects the elderly and immunocompromised populations. Despite the current advancement of treatments, PHN persists in many individuals influencing their daily activities and reducing their quality of life. Anticonvulsants, antidepressants, topical therapies including lidocaine and capsaicin, and opioids, are the most widely used therapies for the treatment of PHN. These medications come with their adverse effects, so they should be used carefully with the elderly or with patients with significant comorbidities. Other measures like botulinum toxin, nerve blocks, spinal cord stimulation, and radiofrequency have also contributed significantly to the management of PHN. However, the efficacy, safety, and tolerability of these invasive methods need to be carefully monitored when administering them. Early diagnosis and early initiation of treatment can reduce the burden associated with PHN. The zoster vaccine has effectively reduced the incidence of HZ and PHN. In this article, we discuss the treatment options available for the management of PHN, mainly focusing on the efficacy and safety of different therapeutic modalities.
Aged ; Analgesics, Opioid ; Anticonvulsants ; Antidepressive Agents ; Botulinum Toxins ; Capsaicin ; Comorbidity ; Early Diagnosis ; Herpes Zoster ; Herpes Zoster Vaccine ; Humans ; Incidence ; Lidocaine ; Nerve Block ; Neuralgia, Postherpetic ; Quality of Life ; Spinal Cord Stimulation

Lingshu: Gudu (Miraculous Pivot: Bone-Length Measurement) is compared with literatures concerning the Shushu (ancient Chinese numerology) of the Qin Dynasty (221 B. C. - 206 B. C. ) and the Han Dynasty (206 B. C.-220 A. D.) in this article. And it is discovered that "the number of heaven and earth" in Yijing (The Book of Change) was implied in the bone-length measurement. The theory of Shushu is hidden in the sized of head, neck, chest, abdomen, back and 4 extremities according to the measurement. The meaning of establishment of bone-length measurement, which is found to have universality, laid in setting down the measurement of meridians. And it is the origin of the proportional measurement of locating acupoints. Checked with the theory of Shushu, errors in the description of bone-length measurement could also be found in Lingshu: Gudu (Miraculous Pivot: Bone-Length Measurement) of the present edition, which is helpful for the modern study on the measurement.
Acupuncture ; history ; Acupuncture Points ; China ; History, Ancient ; Humans ; Medicine in Literature ; Meridians

The function, circulation, distribution and characteristics of Weiqi (Defensive qi) in ancient literatures are retrieved and extracted in this article to analyze and study the theory of "applying acupuncture according to the properties of Weiqi (Defensive qi)" in Internal Classic. The result shows that Deqi (arrival of qi) is closely related with Weiqi (Defensive qi). The circulation of Weiqi (Defensive qi) has its own temporal regularity, which should be followed by the application of acupuncture. The gathering and scattering of Weiqi (Defensive qi) can be used to explain the relationship between distribution of acupoint and curative effect. Circulation of Weiqi (Defensive qi) is influenced by yang qi in the universe which varies according to seasonal factors. Therefore, acupoint selection according to time should not be adopted mechanically. Furthermore, the circulation of Weiqi (Defensive qi) can be either independent or identical with the channels. Therefore, it is still under discussion whether the needling sensation is actually transmit along the channels in the experiment of "propagated sensation along channels".
Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature ; Qi ; Seasons

OBJECTIVETo study the prophylactic effects of nasal tolerance with a dual analogue, Lys262-Ala207, on the mouse model of experimental autoimmune myasthenia gravis (EAMG) and the underlying mechanism.

METHODSMouse model of EAMG was induced by intraperitoneal injection of mAb35. Lys262-Ala207 or PBS was given nasally before 10 days (study group A and control group A) or on the day (study group B and control group B) of immunization for 10 days. Clinical syndromes were evaluated after immunization. Serum level of acetylcholine receptor antibody (AChR-Ab) IgG was detected using ELISA. The number of monouclear cells expressing CD4+ and CD4+ CD25+T from spleen was measured using flow cytometry.

RESULTSCompared with the corresponding control groups, the clinical syndromes were improved (P<0.01) in mice from the study groups A and B. The positive rate of the repetitive nerve stimulation (RNS) test in the study groups A and B was significantly lower than that in the corresponding control groups (P<0.01). The study group A showed lower positive rate of RNS than the study group B (P<0.05). The serum levels of AChR-Ab IgG in the study groups A and B (15.01+/-1.09 and 19.23+/-1.31 microg/mL) decreased compared with that in the corresponding control groups (28.12+/-1.28 and 29.35+/-1.28 microg/mL) (P<0.01). The study group A mice had lower serum AChR-Ab IgG levels than the study group B (P<0.01). The number of CD4+ CD25+T cells in the study groups A (4.516+/-0.598%) and B (3.671+/-0.300%) increased significantly compared with that in the corresponding control groups (2.661+/-0.411% and 2.412+/-0.500%) (P<0.01) and more CD4+ CD25+T cells were found in the study group A than in the study group B (P<0.01).

CONCLUSIONSNasal administration with dual analogues may ameliorate clinical syndromes in EAMG rats, which may be associated with decreased serum AChR-Ab IgG levels and increased number of CD4+ CD25+T cells from spleen.

Administration, Intranasal ; Animals ; Female ; Immune Tolerance ; drug effects ; Immunoglobulin G ; blood ; Mice ; Mice, Inbred C57BL ; Myasthenia Gravis, Autoimmune, Experimental ; immunology ; prevention & control ; Receptors, Cholinergic ; immunology ; T-Lymphocytes, Regulatory ; physiology

To compare the efficacy of interferon and thymosin alpha-1 combination therapy with interferon monotherapy for HBeAg positive chronic hepatitis B. The relevant randomized controlled trials were searched throughout PubMed, EBSCO, Cochrane Library, CBMdisc, VIP, WanFang since Janurary 1990. Studies were included if patients were followed up for at least 6 months after cessation of treatment. Meta-analysis was carried out with RevMan5.0 software. Subgroup analyses were used at different time of observation. Seven randomized controlled trials were included(535 patients in total). According to the results of meta-analysis, the combination therapy was remarkably more effective than monotherapy both at the end of the treatment and the follow-up in terms of HBV-DNA negative rate (54.9% vs 36.3%, OR=2.39, 95% CI=1.64-3.49, P value is less than 0.01; 58.6% vs 30.7%, OR=3.68, 95% CI=2.51-5.41, P value is less than 0.01, respectively), ALT normalization rate (74.5% vs 60.9%, OR=1.94, 95% CI=1.26-3.00, P value is less than 0.01; 74.0% vs 55.6%, OR=2.36, 95% CI=1.54-3.62, P value is less than 0.01, respectively), HBeAg loss rate (56.9% vs 36.7%, OR=2.38, 95% CI=1.61-3.51, P value is less than 0.01; 62.2% vs 33.2%, OR=3.42, 95% CI=2.31-5.06, P value is less than 0.01, respectively) , and HBeAg seroconversion rate (40.1% vs 29.0%, OR=1.65, 95% CI=1.10-2.47, P value is less than 0.05; 47.0% vs 29.5%, OR=2.13, 95% CI=1.43-3.16, P value is less than 0.01, respectively); the HBsAg loss rate of the combination therapy group was significantly higher than that of the monotherapy group only at the end of the follow-up (9.8% vs 3.7%, OR=2.92, 95% CI=1.09-7.76, P value is less than 0.05). Interferon and thymosin alpha-1 combination therapy achieves superior effect with no increase in the adverse effects as compared to interferon monotherapy for HBeAg positive chronic hepatitis B.
Antiviral Agents ; therapeutic use ; Drug Therapy, Combination ; Hepatitis B e Antigens ; blood ; Hepatitis B, Chronic ; blood ; drug therapy ; Humans ; Interferons ; therapeutic use ; Randomized Controlled Trials as Topic ; Thymosin ; analogs & derivatives ; therapeutic use ; Treatment Outcome