OBJECTIVE：To develop a method for the determination of eperisone hydrochloride by high performance liquid chromatography(HPLC).METHOD:C18 column as the stationary phase and methanol-water-triethylamine(80∶19.5∶0.5,V/V,adjusted to pH 7.5 with glacial acetic acid) as the mobile phase were adopted.The detection was performed at 254nm.Eperisone hydrochloride tablets were disssolved in methanol,supersonic-vibrated and filtered.Tolperisone was used as internal standard.RESULTS:The standard curve was linear in the range of 0.4～1.6mg/ml with the excellent correlation coefficient of 0.9999.The average recovery was 98.4%(RSD=1.2%,n=15).Three batches of eperison hydrochloride tablets were analyzed and the labelled contents were 97.8%,98.3% and 98.9% respectively.CONCLUSION:The method was simple,sensitive,rapid and suitable for the quantitative anylysis of the preparation.