Objective: To optimize the processing technology of stir-fried Euodiae Fructus with the water decoction of Coptidis Rhizoma by L9(34) orthogonal design. Methods: The factors including the dosage of Coptidis Rhizoma, stir-frying temperature and stir-frying time, and phenolic acids (neochlorogenic acid, chlorogenic acid, caffeic acid, and cryptochlorogenic acid), flavonoids (rutin, hyperin, and narcissoside), alkaloids (dehydroevodiamine, evodiamine, rutaecarpine, evocarpine, and dihydroevocarpine), and volatile oils in Euodiae Fructus were taken as evaluation indexes to optimize the processing technology. Results: The best processing technology for stir-fried Euodiae Fructus with the water decoction of Coptidis Rhizoma was as follows: the dosage ratio of Euodiae Fructus to Coptidis Rhizoma 10:1, stir-frying temperature 150 ℃, and stir-frying time 8 min. Conclusion: Simultaneously, taking the content of four kinds of components as the indexes, the method for optimizing the optimal processing technology of stir-fried Euodiae Fructus with the water decoction of Coptidis Rhizoma is stable, reliable and feasible.

Objective: To establish fingerprints of Euodiae Fructus in different years by UPLC, analyze them with chemical pattern recognition technology, and determine the content of four quality difference markers, so as to provide new ideas for the research of Euodiae Fructus “Chen Jiuzhiliang” and provide basis for its quality evaluation. Methods: The fingerprints of Euodiae Fructus samples in three different years were established based on UPLC method. The similarity was calculated by the Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine (2012A edition), and the pattern recognition was carried out by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Results: UPLC fingerprints of 47 batches of Euodiae Fructus samples (similarity > 0.997) were established, 40 common peaks were identified and 12 of them were identified. PCA and OPLS-DA analysis were used to effectively distinguish three different years of Euodiae Fructus. A total of 22 quality differential markers were screened, and the content of neochlorogenic acid, chlorogenic acid, evodiamine and rutaecarpine were determined. Conclusion: The combination of UPLC fingerprint of Euodiae Fructus and multi-component chemical pattern recognition technology can effectively distinguish Euodiae Fructus in different years and provide reference for “the older, the better” and quality control of Euodiae Fructus.

Objective To seek a new method to identify viability of myocardium by adenosine stress echocardiography combined with quantitative tissue velocity imaging(QTVI)and tissue tracking(TT).Methods Fifteen healthy canines were selected to establish the models of acute myocardial infarction and reperfusion by ligating anterior descending branch of coronary artery for 90 minutes and then releasing the artery to get reperfusion.After reperfusion.peak velocity in systole(Vs),peak velocity in isovolumic contraction(VIVC)and the displacement in systole(Ds)were measured on anterior wall and anterior septum at baseline.The 2,3,5-triphenyl tetrazolium chloride(TTC)staining was set as a"gold standard"for defining the viable and non-viable groups.The sensitivity and specificity of assessing myocardial viability were determined with QTVI and TT.Comparison of variables between viable and non-viable group was made by using t test.One way analysis of variance and LSD-t test were used to estimate the significance of differences in different states.Results Compared with baseline,Vs,VIVC and Ds decreased significantly(P＜0.01)after reperfusion in both viable and non-viable group.After administration of adenosine,Vs and Ds increased(P＜0.05),but VIVC didn't change significantly compared with that before administration of adenosine in viable group(P＞0.05).Variables in non-viable group didn't change significantly after administration of adenosine(P＞0.05).By receiver operating characteristic(ROC)analysis for predicting myocardial viability,when a cutoff value of △Vs(%)rate was 17.9,the sensitivity and the specificity was 78.6%and 81.1%,respectively,and when the cutoff value of △Ds(%)rate was 18.4,the sensitivity and the specificity was 75.0%,83.6%,respectively.Combined △Vs(%)with △Ds(%),the sensitivity and specificity to prediction of myocardium viability could reach 94.6%and 68.0%,respectively.Conclusions When the viability of myocardium after myocardial infarction is assessed by using the method of adenosine stress echocardiography with QTVI and TT,the sensitivity and the specificity are greatly enhanced.

nd targeted-ultrasound can facilitate VEGF gene transfer of vascular endothelial. Targeted-ultrasound group transfer efficiency is higher than that of microbubble group, and the plasmid alone can not promote transfer of vascular endothelial.

Objective To explore the endogenesis metabolizer character of kidney-yang insufficiency syndrome model rats caused by hydrocortisoni natrii succinas with metabolomics technology. Methods Twenty four Sprague-Dawley(SD)male rats were divided randomly into a normal group, a kidney-yang insufficiency model group, and a oral administration group, eight rats in each group. After producing kidney-yang insufficiency model by injecting hydrocortisoni natrii succinas intramuscularly, oral administration group rats were administered orally with white prepared lateral root of aconite every day for two weeks. After twenty-four hour the last oral administration, the blood plasm were prepared and used for testing endogenesis metabolism with the liquid phase color spectrum-mass spectra (LC/MS) metabolomics technology. Results Apices shape change of lysophosphatidyl choline (LPC) and lysophosphatidyl ethanolamine(LPE) and 468.4m/z unbeknown chemical compound of normal rats were distinct from those of model rats. Above-mentioned chemical compound as chief material symbols of kidney-yang insufficiency syndrome might be farther studied. Conclusion Finding out differential symbol from endogenesis metabolizer with metabolomics technology was redounded to deep exploring the biology essence of traditional Chinese medicine syndrome.

OBJECTIVETo study the flavones of the flowers of Paulownia fortunei.

METHODThe constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.

RESULTThe compounds were identified as: 3'-O-methyldiplacol (1), 6-geranyl-3, 3', 5, 7-tetrahydroxy-4'-methoxy flavanone (2), homoeriodictyol (3), 5, 7, 4'-trihydroxy-3'-methoxy flavone (4), 3'-methoxyluteolin-7-O-beta-D-glucoside (5), apigenin-7-O-beta-D-glucoside (6), kaempferol-7-O-beta-D-glucoside (7), quercetin-3-O-beta-D-glucoside (8), kaempferol-3-O-beta-D-glucoside (9), quercetin-7-O-beta-D-glucoside (10), luteolin-7-O-beta-D-glucoside (11).

CONCLUSIONAll these compounds were obtained from the flowers for the first time.

Flavones ; chemistry ; isolation & purification ; Flowers ; chemistry ; Magnetic Resonance Spectroscopy ; Plants, Medicinal ; chemistry ; Spectrometry, Mass, Electrospray Ionization

Objective　To collect data of fungi isolated from a fungus monitoring network in Hainan Province from 2013 to 2022, and analyze the drug resistance characteristics of Candida and the results of serological tests, with an aim to provide a basis for clinical diagnosis and treatment. Methods　In accordance with the National Fungal Drug Resistance Monitoring Network technical scheme, the qualifying fungal data were extracted from the microbial identification system database using SQL language, and the data information was then analyzed, with statistical processing done using SPSS 26.0 software. Results　Among 5 503 fungal isolates from clinical specimens between 2013 and 2022, cervical orifice secretions accounted for 30.37%(1 671 strains), mid-stream urine for 23.55%(1 296 strains), lower respiratory tract specimens for 25.24% (1 389 strains)[(sputum for 20.37%(1 121 strains) and alveolar lavage fluid for 4.87%(268 strains)], wound pus for 9.59%(528 strains), ascites for 5.60%(308 strains), blood for 3.67%(202 strains), cerebrospinal fluid for 0.38%(21 strains), and joint fluid for 0.04%(2 strains), with the highest number of strains isolated in 2022 and the lowest in 2013, the 2022 figure is about 2.6 times that of 2013. Among yeast-like fungi, Candida albicans had the highest proportion with 3 312 strains accounting for 60.2%; The highest resistance rate of Candida albicans was to fluconazole at 16.7%, with 2.5% being non-wild type (NWT) for amphotericin B; Candida tropicalis had the highest rate of resistance to fluconazole at 36.0%, with NWT at 41.1% for fluconazole and 3.1% for amphotericin B; Candida glabrata had a resistance rate to fluconazole of 2.8%, dose-dependent susceptibility (SDD) of 97.2%, NWT of 15.5% for fluconazole, and NWT of 8.6% for itraconazole; Candida parapsilosis had the highest resistance rate to fluconazole at 15.7% and and NWT of 8.3% for amphotericin B; Candida krusei had a 0.0% resistance rate to caspofungin; and Candida dubliniensis was 100.0% NWT to fluconazole. Of 70 cases of blood culture-positive specimens, 64 cases were detected by G test and 25 cases by Mn test, and the positive blood cultures were statistically significant when compared with the G test and Mn test, respectively (P<0.01). Conclusions　 Fungal serological test can make up for the deficiency of blood culture and distinguish fungal invasion and colonization, thus providing a basis for the effective control of fungal infection in clinical practice.

OBJECTIVETo analyze the results of clinical and bacteriological examinations in patients with crush syndrome who suffered infectious complications after an earthquake in Sichuan, China.

METHODSA total of 313 bacteriological samples among 147 patients with crush syndrome were collected. Infectious complications, results of microbiological examinations, potential risk factors of infection and mortality were analyzed statistically.

RESULTSIn the obtained database, 112 out of the 147 (75.7%) patients had infectious complications, in which, wound infection, pulmonary infection, and sepsis were most common. The time under the rubble and the time from injury to treatment were related to the occurrence of wound infection (P equal to 0.013, odds ratio 2.25; P equal to 0.017, odds ratio 2.31). Sepsis and wound infection were more common in patients who underwent fasciotomy or amputation than in those who did not (P equal to 0.001).

CONCLUSIONQuick rescue and injury treatment can decrease the infection risk in crush syndrome patients. It is better to obtain microorganic proofs before applying antibiotics, and bacteriological and drug sensitivity data should be taken into account, especially considering that most of these infections are hospital-acquired and drug resistance. Emphasizing the accuracy and efficiency of wound management in emergency situations, cautiously assessing the indications for fasciotomy to avoid open wounds from unnecessary osteofascial compartment decompression incisions may decrease the incidence of infection and ameliorate the prognosis.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bacterial Infections ; etiology ; Child ; Child, Preschool ; China ; Crush Syndrome ; complications ; microbiology ; Earthquakes ; Female ; Humans ; Male ; Middle Aged ; Risk Factors

OBJECTIVETo monitor the changes of hemolysis parameters and endothelial cell markers in thrombotic thrombocytopenic purpura (TTP) and reveal the clinical significance of these changes.

METHODSvWF-cleaving protease (vWF-CP) activity in 3 cases of TTP was detected by Western blot. The percentages of fragmented red cells (FRC) were counted throughout the entire clinical course. Levels of plasma thrombomodulin were detected by Western blot combined with density screening in TTP and healthy individuals (n = 3). Concentration of plasma VEGF was measured by enzyme-linked immunosorbent assay in TTP and healthy individuals (n = 9). Fundus fluorescein angiography was performed to search the evidence of microvascular thrombosis in one TTP patient with impaired visual acuity.

RESULTSThe lower vWF-CP activity was observed in TTP patients; the percentages of FRC in 3 cases of TTP were 1.65%, 2.50%, 3.32% respectively with an average of 2.49% at the onset of and decreased with the improvement of the disease. The levels of plasma TM and VEGF were significantly elevated in TTP than those in healthy individuals, and related to the severity of TTP. Fundus photography in one TTP patient with impaired visual acuity revealed vascular occlusion in fundus arteriole and venulae.

CONCLUSIONSA decreased vWF-CP activity is in favour of TTP diagnosis. Dynamic monitoring of plasma TM and VEGF as well as percentages of FRC are useful indexes for reflecting the severity and evaluating therapeutic response of TTP. Selective fundus fluorescein angiography is useful for the judgement of microvascular thrombosis in TTP.

ADAM Proteins ; blood ; ADAMTS13 Protein ; Biomarkers ; blood ; Blotting, Western ; Endothelial Cells ; metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Hemolysis ; Humans ; Male ; Middle Aged ; Purpura, Thrombotic Thrombocytopenic ; blood ; pathology ; Thrombomodulin ; blood ; Time Factors ; Vascular Endothelial Growth Factor A ; blood ; Young Adult

The aim of this experiment is to find a feasible impersonal index for analyzing the physiological mental fatigue level. Three characteristic parameters, relative power in different rhythm, barycenter frequency and power spectral entropy, are extracted from two channels' electroencephalogram (EEG) under two physiological mental fatigue states. Then relationships between such three parameters and physiological mental fatigue are analyzed to explore whether they can be of use for detecting (or monitoring) the mental fatigue level. The experiment results show that the relative power, barycenter frequency and power spectral entropy of EEG exhibit strong correlation with physiological mental fatigue level. While physiological mental fatigue level increases, the relative power in theta, alpha and beta rhythms, barycenter frequency and power spectral entropy of EEG decrease, but the relative power in delta rhythm of EEG increases. The relative power in four rhythms, barycenter frequency and power spectral entropy of EEG reflect the change of physiological mental fatigue level sensitively, and may hopefully be used as indexes for detecting physiological mental fatigue level.
Adult ; Electroencephalography ; Entropy ; Humans ; Male ; Mental Fatigue ; physiopathology ; Signal Processing, Computer-Assisted ; Young Adult