Objective: To observe the effects of acupuncture combined with isokinetic eccentric exercise on pain and motor function in patients with knee osteoarthritis (KOA). Methods: Sixty-four patients with KOA were randomly divided into an observation group and a control group, with 32 cases in each group. The observation group received acupuncture and isokinetic eccentric exercise, and the control group received isokinetic eccentric exercise and oral intake of meloxicam tablets. Both groups were treated for 4 weeks. Before and after treatment, the visual analog scale (VAS) score, the lysholm knee scale (LKS) score, and the quadriceps peak torque (PT) were evaluated. Results: The total effective rate was 93.8％ in the observation group, versus 81.3％ in the control group. The difference between the two groups was statistically significant (P<0.05). After treatment, the VAS scores of the two groups were lower than those before the treatment; both LKS score and quadriceps PT were higher than those before treatment, and the differences were statistically significant (allP<0.05). The VAS score, LKS score and quadriceps PT (angular velocity was 60 °/s) of the observation group were significantly different from those of the control group (all P<0.05). Conclusion: Acupuncture plus isokinetic eccentric exercise is effective in treating KOA. The combined treatment can reduce the pain of KOA patients, improve joint function, strengthen quadriceps, improve joint stability, and its curative effect is better than isokinetic eccentric exercise plus oral intake of meloxicam tablets.

Objective:To investigate the specific stimulation of the B cell epitope peptides of Mycobacterium tuberculosis antigen (Mtb-Ag) on human peripheral γδ T cell proliferation.Methods: We selected the sequences of B cell epitope peptide from Mtb-Ag that were reported in literature and T cell epitope peptide that recently identified in this laboratory to synthesize six peptides of B cell epitopes (BP1-BP6) and two peptides of γδ T cell epitopes (TP14,TP15).The 24-well culture plates were coated with these peptides.The PBMCs were isolated from peripheral blood of healthy individuals and stained with CFSE,followed cultured for 12 days in the IL-2 containing medium.Mtb heat resistant antigen ( Mtb-HAg ) group as positive control and IL-2 only group as negative control.The percentages and proliferation index of γδ T cells were determined by flow cytometry.Results: By using Wilcoxon signed rank test for paired comparison of negative control group,the percentages of γδ T cells in cultured PBMCs with BP2,BP4 and TP14, TP15 and Mtb-HAg increased significantly in 14 samples (P<0.05);and the proliferation index of γδT cell in cultured PBMCs with BP2,BP4,BP5,BP6 and TP14,TP15 increased significantly in 7 samples (P<0.05).Conclusion: Taken together,the B cell epitope peptides from Mtb Antigens are capable of stimulating the γδ T cell proliferation specifically in vitro.Although there was individual difference inγδT cell proliferative response to B cell epitope peptides,these results strongly suggest the B cell epitope peptides also can specifically trigger the TCR ofγδT cells.

This paper analyzes the explore the factors influencing the TCM clinical pathway using the fishbone dragram. We found that the main impacting factors of TCM clinical pathway were government policy factors, hospital management factors and personal factors. It was suggested to promote Chinese medicine clinical pathway pilot job, not only need to cooperate with the administrative departments of health and medical institutions, also need to strengthen the change of the idea of both doctors and patients.

Objective:To understand the construction,functional status and the meaning of p53 gene of PA-1 cells derived from human teratocarcinoma.Methods:DNA basic sequence analysis,FASAY(functional and sequencing analysis of separated alleles in yeast)and Western blot analysis were used.Results:Direct sequence analysis RT-PCR products showed both wild and mutated bands(p53 colon 239 mutation), FASAY showed that one allele of the p53 gene was active(wild) .while the other was inactive( mutant) .In Western blot analysis haven't defect the gene protein of p53, while the PA-1 cells expressed the p21 protein to a lesser extent than normal human tibroblaste. Conclusion:One type of the p53 allele gene of PA-1 cells of human teratocarcinoma was detected as missense mutation after cultured for more than twenty years, and this mutation is not sufficient to induce the instabih'ty of the chromosome of cell line. So that the study for the maintenance contruction of the stability nuclein type of PA-1 cells is very important to determin whether the genes is stability or instability of chromosomes.

Objective To investigate a new method in correction of epicanthus.Methods Asymmetrical Z-plasty was used in correcting epicanthus and lengthening palpebral fissure in 42 cases with 84 eyes.It could be combined with double-eyelid-blepharoplasty to create beautiful eyes.Results All 42 cases with 84 eyes had satisfactory outcome after half a year to 2 years follow-up.The epicanthus scar was conspicuous in the first 6 weeks and it subsided gradually.In the three cases.the hypertrophic scar occurred in the epicanthus,but it softened and became inconspieuous 6 mouths after operation.Conclusion Asymmetrical Z-plasty is a new and reliable method for correction of epicanthus with inconspicuous scar and little chance of recurrence that can be widely used.

Metabolic bone disease(MBD) in preterm infant is a disorder of calcium and phosphorus metabolism that leads to a decrease in bone mineral content, resulting in clinical, biochemical, and imaging changes.It occurs mostly in very low birth weight and extremely low birth weight newborns.The clinical symptoms usually occur from 6 to 12 weeks after birth, mainly manifested as dyspnea accompanied by mechanical ventilation for a long time, rickety-like changes, and even fracture in severe cases.At present, diagnosis of MBD is characterized by biochemical markers, radiology and ultrasound.As the clinical manifestations of MBD in prematurity occur late, early screening and prevention for high-risk groups play an important role to reduce the risk of MBD.

According to the present situation on purchase, management and the use of medical consumables materials, medical consumables catalogue is compiled, bound and allocated to the institution and other offices, thus making the purchase information open and clean, the management standardized. It is proved to be feasible.

From the connotation of the cultural quality and according to the characteristics of evaluation system in college students,the article analyzed the evaluation system of students' culture quality by using balanced scorecard to promote college students' cultural quality education.

Vascular morphology and function changes exist in degenerative retinopathy,which have an important influence on the development and prognosis of the disease.The literature has been reported that retinal vascular structure and function change can be found early by using the retinal oximeter,color Doppler flow imaging,ocular pulse amplitude and so on,as well as exploring the primary and secondary factors affecting the development of retinal blood vessels.This article reviews the vascular behavior characteristics in degenerative retinopathy and their influence factors.

The graduates of Traditional Chinese Medicine Institutions face greater pressure in recent years' severe employment situation.It is an effective way to develop career education for students to achieve a good career planning and placement.The paper mainly discusses the status of career education in the Traditional Chinese medicine institutions,the mode establishment,the core latitude and the significance of career education.