Adult Clonorchis sinensis were collected from the biliary passage of nine cases of medico-legal autopsies. The worms were washed repeatedly with tap water and rinsed twice with physiological saline. Incubation took place in Tyrode's medium in order to recover live worms. The number of worms surviving in the medium was determined. The rate of surviving worms showed an unexpectedly high percentage (22.8%) in those cases autopsied 2 days after death. The surviving worm burden dropped sharply to 6.3 and 9.2 percent for those samples taken 5 days after death.
parasitology-helminth-trematoda ; Clonorchis sinensis ; autopsy

Twenty-five paraffin-embedded tumor tissues were analyzed for detection of HPV 16 and 18 in cervical adenocarcinoma by polymerase chain reaction with type specific primers and by non-radioactive Southern blot hybridization for confirmation . The suitability of paraffin-embedded tissue as PCR material was confirmed by successful amplification of 100% of cervical specimens with human -globin specific primer. Eighty four percent of the cervical adenocarcinoma tissues were positive for HPV 16 and/or 18. HPV 16 positive rate was 68%, HPV 18 was 60%. The double infection with HPV 16 and 18 was found in 44%. Three cases of the negative specimen in PCR for each type of HPV DNA 16 and 18 were positive in Southern blot hybridization. The total positive rate was 92% for HPV 16 and/or HPV 18, HPV 16 positive rate was 80%. HPV 18 was 72%. The double infection with HPV 16 and 18 was 60%. These results suggest that the pattern of HPV types 16 and 18 is closely associated with carcinogenesis of cervical cancers. HPV type 18 appears to be preferentially related to cervical adenocarcinoma and the poor prognosis of these patients. Therefore, determination of HPV DNA type in cervical carcinoma patients is important in treatment and prognosis.
Humans ; Adenocarcinoma

Sparganum infestation in human body was reported over 20 cases in Korea. Infestation of sparganosis in spermatic cord was reported a case in Korea. Three cases of sparganosis at Keu Je island in Korea was reported by Dr. Weinstein, Krawczyc and Peers 1954. We experienced a case of the sparganum infestation in scrotum. The patient, 41 year-old male who was suffering with pulmonary tuberculosis moderate advanced, visited 3rd Army Hospital urologic clinic complained with acute swelling of the scrotum in baby head size abruptly without any specific causes. severe tenderness and walking disturbance. The laboratory findings revealed no specific findings except ascaris ova in stool. Operation revealed massive hematoma formation and proliferated connective tissue where noted several infested parasites in length of 120cm, 75cm, 70cm around the tunica vaginalis in scrotum Extracted parasites revealed milkish-whitish color, flat, fragile, and tendon like glistening appearance. There is not any specific sign and symptoms by parasite infestation on the other organs. Patient had an episode of intake of raw snake as a tonic 9 years ago at Keu Je island in Korea.
Adult ; Ascaris ; Connective Tissue ; Head ; Hematoma* ; Hospitals, Military ; Human Body ; Humans ; Korea ; Male ; Ovum ; Parasites ; Scrotum* ; Snakes ; Sparganosis* ; Sparganum ; Spermatic Cord ; Tendons ; Tuberculosis, Pulmonary ; Walking

No abstract available.
Cryptococcosis* ; Hybridomas* ; Immunity, Cellular* ; T-Lymphocytes*

No abstract available.
Cryptococcosis* ; Hybridomas* ; Immunity, Cellular* ; T-Lymphocytes*

Dieulafoys lesion consists of abnormally large gastric submucosal artery which ruptures into the stomach causing massive or recurrent intragikstric bleeding. The lesion is very small and easily overlooked even at laparatomy and aan only be correctly diagnosed by endoscopy or arteriography if the patient is actively bleeding. Three patients who were admitted with bleeding of upper gastrointestinal tract and eventually diagnosed as having Dieulafoys lesions were analysed. All were men with age range of 44 to 55 years. All patient were asymptomatic before presenting with hematemesis. Two of the three patients had had history of upper Gl bleeding. One patient used analgesics daily for ureteral colic and two patient drank alcohol excessively. Gastroscopy was performed during the bleeding episode in all three patients. Dieulafoy's lesion was seen in all three cases and in the second case, there was concomittent diffuse petechia in the whole stomach. The lesion was situated on the posterior wall of upper body in one, on anterior wall of upper body in another, lesser curvature side of gastric fundus in the other case. All three patient underwent laparotomy for persistent bleeding and the lesion was suture ligated only in two patients while in one patient vagotomy and pyloroplasty was added. Resection biopsy was performed in two cases and both revealed only normal gastric mucosa. All patients discharged after complete recover.
Analgesics ; Angiography ; Arteries ; Biopsy ; Endoscopy ; Gastric Fundus ; Gastric Mucosa ; Gastroscopy ; Hematemesis ; Hemorrhage ; Humans ; Laparotomy ; Male ; Renal Colic ; Rupture ; Stomach ; Sutures ; Upper Gastrointestinal Tract ; Vagotomy

A study was required to evaluate the prevalence of clonorchiasis among school children in the 7 differnet schools located near the Gumho basin and to determine the effectiveness of the anticlonorchis campaign conducted in that area. Surveys for Clonorchis sinensis and the worm burden in the same areas were compared for the period 1960-1970. Both surveys concentrated on children in grades 1-3 ranging from 6-11 years old. They were examined using primarily intradermal tests with Clonorchis antigen and followed by stool examinations. Stool examinations consisting of the formalin-ether sedimentation(Ritchie, 1948 ) and the Stoll egg-count techniques were performed in those cases which revealed positive and doubtful intradermal reactions. The overall prevalence rate for clonorchiasis was reduced from 13.4 per cent to 4.1 per cent. There was also a significant reduction in the worm burden, suggesting that no clonorchiasis will be found among chileren in the near future. In connection with this survey, the prevalence rate for intestinal helminths in 1960 was compared with 1970. The rate for Ascaris lumbricoides had decreased, while the rates for Trichuris trichiura and Trichostrongylus species had increased. No fluctuation in the rate of hookworm was observed during the past 10 years. Clearly, the present survey denotes a requirement for a combined procedure of health education and mass survey with intradermal testing. It has been found that these methods not only greatly reduce the prevalence of clonorchiasis but also is followed by the marked reductions of the worm burdens of the children examined.
parasitology-helminth-trematoda-nematoda ; Clonorchis sinensis ; Ascaris lumbricoides ; Trichuris trichiura ; Trichostrongylus species ; epidemiology

No abstract available.
HIV-1* ; Humans* ; Tumor Necrosis Factor-alpha*

No abstract available.
Arthritis, Rheumatoid* ; Humans

No abstract available.
Leg* ; Myocutaneous Flap* ; Superficial Back Muscles*