Angiogenesis is an important step during the occuring, development and metabsis of tumor which is regulated by some factors. PLGF is one of the VEGF family. It is homologous with VEGF-A, VEC,-FR-1 is its receptor. Although it expresses weakly during physiology. PLGF is an important pathologic angio-genesis factor at the condition such as anoxia, truma or tumor. Becanse its expression becomes strong at path-ologic cyophoria and many kinds of tumors, PLGF has a good value in clinic reasereh.

Objectives: To reveal the possible role and mechanism of disc-facet angle in the occurrence and progress of Hirayama disease by analyzing the correlation of clinical appearance and the variation of disc-facet angle in Hirayama disease. Methods: 45 patients with Hirayama disease treated in the orthopedic de-partment of Peking University Third Hospital from October 2006 to January 2012 were reviewed. There were 44 males(97.8%) and 1 female(2.2%) with an average age of 19.33±3.89(range 13-37) years old. The onset age was 16.33±2.73 (range 10-27) years old and the duration of history was 35.64±23.24 (range 1 to 120) months. The disc-facet angle of C3-T1 was measured on PACS system. An extension line was made along the upper edge of objective vertebral body on the CT scan sagittal reconstruction image. An extension line was made along the joint surface of upper articular process of objective vertebra on the sagittal plane which crossed the midpoint of the facets. The blunt angle between two lines was the disc-facet angle. The cases were divided into unilateral affect and bilateral affected group according to history, spinal cord function and spinal cord lesion level. The correlation of above data and the variation of disc-facet angle were analyzed. Results: The duration of history in unilateral affected patients was significantly shorter than that of bilateral affected patients (P<0.05). The duration of history in sequential onset patients was significantly shorter than that of simultaneous onset in bilateral affected patients (P<0.05). The JOA score 17 and Chinese score 40 in unilateral affected patients were higher than those of bilateral affected patients. The C3-C7 disc-facet angle of affected side was bigger than that of normal side in unilateral affected patients, and C5, C6 had statistically significant difference (P<0.05). The spinal cord lesion level was mainly at C5 and C6 in unilateral affected group. The disc-facet angle had no difference on both sides in bilateral affected patients but the figures at C4 and C5 level were bigger than that of normal side in unilateral affected patients. The spinal cord lesion level was mainly at C4 and C5 in bilateral affected group. The stability of upper cervical spine segments was even worse in unilateral affected group and resulting in higher level of spinal cord lesion comparing with bi-lateral affected group. Conclusions: The disc-facet angle of C4, C5 is bigger in bilateral affected patients and may be correlated with higher level spinal cord lesion and more severe clinical symptoms. The C5, C6 disc-facet angle of affected side is significantly bigger than that of normal side in unilateral affected patients. This may be the key factor of spinal cord lesion mainly at C5 and C6 level on the same side.

Objectives: To study the morphological difference of luscka joints between Hirayama disease patients and non-Hirayama disease patients on CT scan and to provide a new possible mechanism of Hirayama disease. Methods: 32 patients(all males) with a mean age of 19.4±4.1(range 16-37 years) and with Hirayama disease were treated in our hospital from October 2006 to January 2012, the mean course of disease was 31.7±23.7 months(range, 1-120 months). 32 patients(all males) with a mean of age was 19.1±4.3 (range, 12-26 years) suffering from acute neck pain and having no Hirayama disease were reviewed as control. Both groups showed no age related difference. From the cervical CT coronal plane reconstruction images which passing through the transverse foramen center of C3-C7 in GE-PACS system, the following data were measured in both sides: ①The width of the uncinate process base: the distance between inner and outer margin of the uncinate process at the upper edge of the vertebral body. ②The height of the uncinate process:the vertical distance from the top of the uncinate process to the upper edge of the vertebral body. ③The distance between two uncinate processes: the distance between the tips of the bilateral uncinate processes. ④The inclination angle of the uncinate process: the angle between the uncinate process and the upper edge of the vertebral body. ⑤The inclination angle of the inferior endplate: the angle between the uncinate process:the vertical distance from the top of the uncinate process to the upper edge of the vertebral body. Results:There were no significant side-related differences on the width of the uncinate process base, the height of the uncinate process, the distance of the uncinate process, the inclination angle of the uncinate process and the inclination angle of the inferior endplate at the same segment from C3 to C7 in Hirayama disease patients(P>0.05). However, differences were found on the height of the uncinate process and the distance between two uncinate processes of C3-C7 (P<0.05), C6 and C4 had the highest and lowest height of the uncinate process as C6>C5>C7>C3>C4. The distance of the uncinate process gradually increased from C3 to C7. There were no significant differences on the width of the uncinate process base, the inclination angle of the uncinate pro-cess and the inclination angle of inferior endplate(except for C3) of C3-C7(P>0.05). Then, using the mean value of the left and right sides as the width of the base of uncinate process, the height of uncinate process, and calculating the sum of inclination angle of the uncinate process, the sum of inclination angle of inferior endplate of the upper vertebra and the difference between the sums (the sum of inclination angle of uncinate process - the sum of inclination angle of inferior endplate of the upper vertebra), then calculating the mean value and standard deviation. Compared with the non-Hirayama disease patients, there were no significant dif-ferences on the uncinate process base, the distance of the uncinate process and the sum of inclination angle of the uncinate process at the same segment of Hirayama disease patients (P>0.05), while the height of the uncinate process and the sum of inclination angle of uncinate process of Hirayama disease patients were sig-nificantly smaller than those of the control group, respectively(P<0.05), and the differences between the sums were larger than those of the control group (P<0.05). Conclusions: Hirayama disease patients may possess a dysplasia in the luscka joint, manifesting the nonuniform development of the uncinate indicators. Lower unci-nate process and smaller inclination angle of inferior endplate of the upper vertebra are common, The conse-quential cervical instability may play a significantly important role in the pathogenesis and progress of Hi-rayama disease.

The curative effects of multi-disciplinary and comprehensive treatments for colorectsl cancer have been widely recognized in recent years.Because of the diversification of colorectal cancer treatment and the relatively mature technology,the current treatment of colorectal cancer could embody the multi-disciplinary and comprehensive treatment.The curative effect of neo-adjuvant chemoradiotherapy has been validated by several recent clinical trials.Other treatment methods such as Chinese medicine,radiofrequency ablation and hepatic artery infusion therapy are also involved in the comprehensive treatment,which provide considerable curative effects.

[Objective]To compare the early clinical outcome of ACL reconstruction using the ligament advanced reinforcement system and the ?-ray irradiation allograft.[Method]From January 2006 to January 2008,55 cases of ACL reconstruction were studied.According to the indication of LARS ligament and allograft,suggestion from surgeon and patient's aspiration,one of them were chosen as grafts for ACL reconstruction,including 25 LARS ligaments with titanium screw fixation and 19 allografts with absorptive screw fixation.After reconstruction,both groups of patients carried on functional exercise.Each patient was evaluated with Lysholm-Tegner score and KT-2000 measurements preoperatively and postoperatively at 3 months,6 months,9 months,12 months,15 months and 18 months.[Result]Fifty-two cases were followed up,3 cases were dismissed.The Lysholm -Tegner score in cases using the LARS ligament were higher than that in cases using the allograft 3 months post-operation,6 months post-operation,9 months post-operation.Contemporary comparison of the score gap between the LARS ligament group and the allograft group showed a trend of decrease.During the follow up,the allograft group displayed significantly more laxity in KT-1000 measurements at all time points than the LARS ligament group.And the measurements gap between the two groups showed a trend of increase.[Conclusion]The knee anterior-posterior stability of the patients using the LARS ligament were better than that of patients using the allograft.The knee function of the patients using the LARS ligament recovered earlier than that of patients using allograft at nonage,but long-term effect is almost the same.

No abstract available.
Evoked Potentials, Auditory, Brain Stem* ; Membranes* ; Otitis Media* ; Otitis*

No abstract available.
Anesthesia* ; Animals ; Guinea Pigs* ; Guinea*

No abstract available.
Electric Stimulation* ; Evoked Potentials, Auditory, Brain Stem* ; Spiral Ganglion*

OBJECTIVE: To study flavonoids from the leaves of Zelkova serrata. METHODS: The leaves were extracted with 70% ethanol by continuous thermal reflux. The extract was separated and purified by silica gel column chromatography, polyamide column chromatography, preparative TLC and other isolation techniques. Their structures were identified by their physical properties and spectroscopic data. RESULTS: Eight flavonoids were isolated and identified as myricetin (1), dihydromyricetin (2), quercetin (3), myricitrin(4), myricetin-3-O-β-D-xyloside(5), quercetin-3-O-β-D-xyloside(6), quercitrin(7), and rutin(8). CONCLUSION: All compounds were isolated from Schneider Zelkova leaves for the first time.

BACKGROUND: The sucrose is an ideal material for auxiliary support and mold fil ing that is suitable for application in the medical field with three-dimensional (3D) printing. In China, there are many studies about the 3D printing with sucrose, but the accuracy and porosity of the stent are stil unclear. OBJECTIVE: To explore the technological parameters of the sucrose scaffold by 3D printing technology. METHODS: From the perspective of physical and chemical properties, the sucrose viscosity and thermal decomposition with the change of temperature were analyzed. Based on the pneumatic controlled FDM technology, the mature sucrose scaffold was obtained by researching the match of temperature and pressure, layer setting, as wel as the match of speed and pressure. Then the scaffold was demarcated with microscope, and the porosity was measured by immersion in absolute ethyl alcohol. RESULTS AND CONCLUSION: The sucrose was completely melted at 180 oC, with the biggest liquidity. When the temperature was over 195 oC, the caramel reaction occurred. As the temperature increased, the sucrose viscosity decreased. The optimal molding parameters of the sucrose scaffold with pneumatic control-based 3D printing were 170 oC-0.2 MPa-12 mm/s (temperature-pressure-printing speed). The line width and mean porosity of the wel -shaped sucrose scaffold were 700 μm and 81.893%, respectively.