1.Biointerface Engineering.
Journal of the Korean Medical Association 2001;44(7):710-715
No abstract available.
4.Staphylococcus lugdunensis in Acute Oral Infection.
Kang Ju KIM ; Chong Pyoung CHUNG ; Yong Ouk YOU ; Byung Moo MIN
Journal of the Korean Society for Microbiology 1998;33(6):537-546
To investigate the pathogenicity, genomic pattern, and o-like hemolysin of Staphylococcus lugdunensis (S. lugdunensis) in acute oral infection, S. lugdunensis was isolated from patients with an acute oral infection and from healthy persons. Antibiotic susceptibility, in vitro cellular toxicity, in vivo virulence, and hemolytic activity were tested, and plasmid DNA and restriction pattern of whole genomic DNA were analyzed to characterize the staphylococci. The dot blot and Southern blot hybridization analysis of staphylococcal DNA were performed with o-hemolysin gene probe. The isolation ratio of S. lugdunensis in the patients was higher than that in the healthy persons. S. lugdunensis from the patients with an acute oral infection showed resistance to penicillin, ampicillin, methicillin, cephalothin, and clindamycin. In the analysis of plasmid, there was a clear band about 6.5 kb in three strains of S. lugdunensis isolated from the patients with infection. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had o-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with Staphylococcus aureus (S. aureus) on sheep blood agar plates. In the analysis of genomic pattern, four strains of S. lugdunensis that gave synergistic hemolysis with S. aureus showed a similar genetic pattern with HindIII enzyme digests. In dot blot analysis, all strains of S. lugdunensis showed a positive reaction with the probe of 5-hemolysin gene in S. aureus. In Southern blot analysis, a 7.3 kb HindIII fragment was observed in DNA of S. lugdunensis that gave synergistic hemolysis with S. aureus, and a 2.5 kb band was observed in HindIII digests of S. aureus in the patients. These results suggest that S. lugdunensis may be an important pathogen in an acute oral infection and the 7.3 kb HindIII fragment from S. lugdunensis DNA may contain o-like hemolysin gene.
Agar
;
Ampicillin
;
Blotting, Southern
;
Cephalothin
;
Clindamycin
;
DNA
;
Erythrocytes
;
Hemolysis
;
Humans
;
Methicillin
;
Penicillins
;
Plasmids
;
Sheep
;
Staphylococcus aureus
;
Staphylococcus lugdunensis*
;
Staphylococcus*
;
Virulence
5.The effect of minocycline-ioaded polycaprolactone film to the subgingival microflora of adult periodontitis.
Wone Kyeong KIM ; Seo Young JEONG ; Chong Pyoung CHUNG ; Sang Mook CHOI
Journal of the Korean Society for Microbiology 1992;27(1):7-17
No abstract available.
Adult*
;
Chronic Periodontitis*
;
Humans
6.The effect of minocycline-ioaded polycaprolactone film to the subgingival microflora of adult periodontitis.
Wone Kyeong KIM ; Seo Young JEONG ; Chong Pyoung CHUNG ; Sang Mook CHOI
Journal of the Korean Society for Microbiology 1992;27(1):7-17
No abstract available.
Adult*
;
Chronic Periodontitis*
;
Humans
7.Clinical and microbiological investigation on the effect of doxycycline administration in the management of adult periodontitis.
Mok Hoon OH ; Chong Pyoung CHUNG ; Seong Heui SON ; Soo Boo HAN ; Sang Mook CHOI
Journal of the Korean Society for Microbiology 1992;27(5):457-470
No abstract available.
Adult*
;
Chronic Periodontitis*
;
Doxycycline*
;
Humans
8.The effects of Magnoliae cortex and Zea Mays L. extract mixtures on experimentally induced periodontitis of beagle dog.
Tae Il KIM ; Chong Pyoung CHUNG ; Young KU
The Journal of the Korean Academy of Periodontology 2002;32(4):847-855
It has been reported that Magnoliae cortex extract has antibacterial and antimicrobial activity against pathogenic microbes and Zea Mays L. extract is effective for improving gingival tissue health. The purpose of this study was to examine the anti-inflammatory and antimicrobial effects of Zea Mays L. and Magnoliae cortex extract mixtures through experimental periodontitis induced beagle dog model. Nine beagle dogs with experimentally induced periodontitis were selected. Baseline clinical indices which includes plaque index, gingival index, probing pocket depth, clinical attachment level, gingival fluid flow rate were recorded and microbial assays were done. Magnoliae cortex and Zea Mays L., mixed at 2:1 ratio in 105mg capsular dosage, were taken by 3 capsule (Group I) or 6 capsule dosages (Group II) three times a day. After 4,8,12 weeks, clinical indices were recorded. All data of clinical indices were compared through one-way ANOVA with 95% confidence level. Clinical indices of group I and II showed significantly better results than those of control group. There were no significant differences between group I and II. In conclusion, it was confirmed that mixture of Magnoliae cortex and Zea Mays L. (mix ratio 2:1) possessed clinical improving effects to periodontitis.
Animals
;
Dogs*
;
Magnolia*
;
Periodontal Index
;
Periodontitis*
;
Zea mays*
9.Study on the correlation of periodontopathic microflora and gingival crevicular fluid cytokine on periodontal disease progression.
Hae Joon LEE ; Chong Pyoung CHUNG ; Soo Boo HAN ; Seong Heui SON ; Sang Mook CHOI ; Sam Pyo HONG
Journal of the Korean Society for Microbiology 1993;28(1):81-93
No abstract available.
Gingival Crevicular Fluid*
;
Periodontal Diseases*
10.Evaluation of biodegradability and tissue regenerative potential of synthetic biodegradable membranes.
Dong Kyun KIM ; Young KU ; Yong Moo LEE ; Chong Pyoung CHUNG
The Journal of the Korean Academy of Periodontology 1997;27(1):151-163
The purpose of this study was to evaluate on the biodegradability, biocompatibility and tissue regenerative capacity of synthetic biodegradable membranes-Resolut(R), Guidor(R) and Biomesh(R). To evaluate the cell attachment on the membranes, in vitro, the number of gingival fibroblasts attached to each membrane was counted by hemocytometer. Cytotoxicity test for the membranes was performed by MTT test with gingival fibroblast For evaluation of guided bone regenerative potential, the amount of new bone formation in the rat calvarial defects(5mm in diameter) beneath the membranes was observed for two weeks and examined of the specimens by Massons trichrome staining. Biodegradability was observed for 2, 4, 8 and 12 weeks after implantation of each materials under the skin of rats and examined the specimens with H & E staining. The number of cell attachment were the greatest in Biomesh(R) and followed by Resolut(R). Cell viability of three membranes was almost similar levels. Biodegradability of Resolut(R) was the highest among three membrane and the potential of guided bone regeneration was the greatest in the Biomesh(R) and Resolut(R) was followed. These results suggested that commercially available biodegradable membranes were non-toxic and highly potential to guided bone regeneration.
Animals
;
Bone Regeneration
;
Cell Survival
;
Fibroblasts
;
Membranes*
;
Osteogenesis
;
Rats
;
Skin