1.Biointerface Engineering.
Journal of the Korean Medical Association 2001;44(7):710-715
No abstract available.
4.Staphylococcus lugdunensis in Acute Oral Infection.
Kang Ju KIM ; Chong Pyoung CHUNG ; Yong Ouk YOU ; Byung Moo MIN
Journal of the Korean Society for Microbiology 1998;33(6):537-546
To investigate the pathogenicity, genomic pattern, and o-like hemolysin of Staphylococcus lugdunensis (S. lugdunensis) in acute oral infection, S. lugdunensis was isolated from patients with an acute oral infection and from healthy persons. Antibiotic susceptibility, in vitro cellular toxicity, in vivo virulence, and hemolytic activity were tested, and plasmid DNA and restriction pattern of whole genomic DNA were analyzed to characterize the staphylococci. The dot blot and Southern blot hybridization analysis of staphylococcal DNA were performed with o-hemolysin gene probe. The isolation ratio of S. lugdunensis in the patients was higher than that in the healthy persons. S. lugdunensis from the patients with an acute oral infection showed resistance to penicillin, ampicillin, methicillin, cephalothin, and clindamycin. In the analysis of plasmid, there was a clear band about 6.5 kb in three strains of S. lugdunensis isolated from the patients with infection. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had o-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with Staphylococcus aureus (S. aureus) on sheep blood agar plates. In the analysis of genomic pattern, four strains of S. lugdunensis that gave synergistic hemolysis with S. aureus showed a similar genetic pattern with HindIII enzyme digests. In dot blot analysis, all strains of S. lugdunensis showed a positive reaction with the probe of 5-hemolysin gene in S. aureus. In Southern blot analysis, a 7.3 kb HindIII fragment was observed in DNA of S. lugdunensis that gave synergistic hemolysis with S. aureus, and a 2.5 kb band was observed in HindIII digests of S. aureus in the patients. These results suggest that S. lugdunensis may be an important pathogen in an acute oral infection and the 7.3 kb HindIII fragment from S. lugdunensis DNA may contain o-like hemolysin gene.
Agar
;
Ampicillin
;
Blotting, Southern
;
Cephalothin
;
Clindamycin
;
DNA
;
Erythrocytes
;
Hemolysis
;
Humans
;
Methicillin
;
Penicillins
;
Plasmids
;
Sheep
;
Staphylococcus aureus
;
Staphylococcus lugdunensis*
;
Staphylococcus*
;
Virulence
5.The effects of Magnoliae cortex and Zea Mays L. extract mixtures on experimentally induced periodontitis of beagle dog.
Tae Il KIM ; Chong Pyoung CHUNG ; Young KU
The Journal of the Korean Academy of Periodontology 2002;32(4):847-855
It has been reported that Magnoliae cortex extract has antibacterial and antimicrobial activity against pathogenic microbes and Zea Mays L. extract is effective for improving gingival tissue health. The purpose of this study was to examine the anti-inflammatory and antimicrobial effects of Zea Mays L. and Magnoliae cortex extract mixtures through experimental periodontitis induced beagle dog model. Nine beagle dogs with experimentally induced periodontitis were selected. Baseline clinical indices which includes plaque index, gingival index, probing pocket depth, clinical attachment level, gingival fluid flow rate were recorded and microbial assays were done. Magnoliae cortex and Zea Mays L., mixed at 2:1 ratio in 105mg capsular dosage, were taken by 3 capsule (Group I) or 6 capsule dosages (Group II) three times a day. After 4,8,12 weeks, clinical indices were recorded. All data of clinical indices were compared through one-way ANOVA with 95% confidence level. Clinical indices of group I and II showed significantly better results than those of control group. There were no significant differences between group I and II. In conclusion, it was confirmed that mixture of Magnoliae cortex and Zea Mays L. (mix ratio 2:1) possessed clinical improving effects to periodontitis.
Animals
;
Dogs*
;
Magnolia*
;
Periodontal Index
;
Periodontitis*
;
Zea mays*
6.The effect of minocycline-ioaded polycaprolactone film to the subgingival microflora of adult periodontitis.
Wone Kyeong KIM ; Seo Young JEONG ; Chong Pyoung CHUNG ; Sang Mook CHOI
Journal of the Korean Society for Microbiology 1992;27(1):7-17
No abstract available.
Adult*
;
Chronic Periodontitis*
;
Humans
7.The effect of minocycline-ioaded polycaprolactone film to the subgingival microflora of adult periodontitis.
Wone Kyeong KIM ; Seo Young JEONG ; Chong Pyoung CHUNG ; Sang Mook CHOI
Journal of the Korean Society for Microbiology 1992;27(1):7-17
No abstract available.
Adult*
;
Chronic Periodontitis*
;
Humans
8.Clinical and microbiological investigation on the effect of doxycycline administration in the management of adult periodontitis.
Mok Hoon OH ; Chong Pyoung CHUNG ; Seong Heui SON ; Soo Boo HAN ; Sang Mook CHOI
Journal of the Korean Society for Microbiology 1992;27(5):457-470
No abstract available.
Adult*
;
Chronic Periodontitis*
;
Doxycycline*
;
Humans
9.Study on the correlation of periodontopathic microflora and gingival crevicular fluid cytokine on periodontal disease progression.
Hae Joon LEE ; Chong Pyoung CHUNG ; Soo Boo HAN ; Seong Heui SON ; Sang Mook CHOI ; Sam Pyo HONG
Journal of the Korean Society for Microbiology 1993;28(1):81-93
No abstract available.
Gingival Crevicular Fluid*
;
Periodontal Diseases*
10.The biological effects of fibronectin typeIII 7-10 to MC3T3-E1 osteoblast.
Jeong Ug HONG ; Sang Mook CHOI ; Soo Boo HAN ; Chong Pyoung CHUNG ; In Chul RHYU ; Yong Moo LEE ; Young KU
The Journal of the Korean Academy of Periodontology 2002;32(1):143-160
No abstract available.
Cell Differentiation
;
Cell Proliferation
;
Fibronectins*
;
Osteoblasts*
;
Titanium