No abstract available.
Humans ; Infant* ; Infant, Low Birth Weight* ; Infant, Newborn

No abstract available.
Bacteria*

No abstract available.

BACKGROUND: In the past patients who needed ventilatory care were treated in the hospital but now, thanks to advanced technology, those patients can be treated at their homes. This study was done to evaluate the clinical course of each patient and the effectiveness of home ventilatory care program. METHODS: We reviewed ten cases of ventilator - dependent children who were discharged from the pediatric intensive care unit since we started a home ventilatory care program. RESULTS: Six patients remained ventilator - dependent, three patients died and one patient cannot be reached. The clinical courses before home ventilatory care was decided were diverse. None of the patients needed hospital care related to complications of mechanical ventilatory care. CONCLUSIONS: Despite the small number of patients and the short follow up duration, we concluded that ventilator-dependent children can be successfully managed at their homes.
Child ; Follow-Up Studies ; Humans ; Intensive Care Units ; Respiration, Artificial* ; Ventilators, Mechanical

Although the decreased susceptibility of gonococci to penicillin and tetracycline is a worldwide problem in the treatment of gonorrhea, the gonococci in the West Pacific region are particulary notorious in their resistance. Using a plate dilution method, susceptibility of the gonococci isolated at this institution during 1970 to 1975 was tested to penicillin and tetracycline, which are the most widely used antibiotics for the treatment of gonorrhea. The data of this susceptibility, together with that of the strains isolated during 1966 to 1969 from prostitutes, were analyzed and herewith reported. The range of minimum inhibitory concentration (MIC) of penicillin was 0.01 to 2.0U/ml. Among the 191strains, 87.9% required MIC of 1.0U/ml and over, and 29.3% required 1.0U/ ml and over. The range of MIC of tetracycline was from 0.125 to over 2 microgram/ml. Among the 120 strains, 60% required MIC of 1 microgram/ml and over. This in vitro evidence indicates wide prevalence of less susceptible strains which are difficult to cure with conventional doses of penicillin or with tetracycline. Comparison of the degree and the frequency of less susceptible strains by the year of isolation showed some variation, which may however have been induced by the difference of sources, rather than by the difference of time of isolation.
Neisseria gonorrhoeae/drug effects* ; Penicillins/pharmacology* ; Tetracyclines/pharmacology*

PURPOSE: In colon cancer, the most frequent genetic alteration is found in p53 tumor suppressor gene residing on the short arm of chromosome 17. In order to investigate the significance of wild-type p53, we transfected wild type p53 into human colon cancer cell lines and analysed tbeir biologic effects. MATERIALS AND METHODS: For analysis of p53 status in cell lines, polymerase chain reaction-single stranded confonnation polymorphism (PCR-SSCP), PCR-direct sequencing and Western blot analysis were employed. Transient transfection with liposome-p53 complex was followed by cell biologic assay. RESULTS: We found that twelve of fifteen human colon cancer cell lines showed mutation of p53 by PCR-SSCP method. These results almost corresponded to p53 protein accumulations assessed by Westem blot using PAbl801. After transfection with lipafect- AMINE and wild type p53 complex on p53 mutant type cell line (LS1034), viability was reduced to 17.9%, and invasiveness was reduced to 37.3%. Morphologically, wild type p53 transfected cells showed lumen formation and apoptosis after induction of differentiation by Matrigel. CONCLUSION: Wild type p53 transfection into p53 mutated colon cancer ceil line resulted in restoration of tumor suppressor effect of p53, and this model would be one of the experimental systems for p53-based gene therapy.
Apoptosis ; Arm ; Biological Assay ; Blotting, Western ; Cell Line* ; Chromosomes, Human, Pair 17 ; Colon* ; Colonic Neoplasms* ; Genes, p53* ; Genes, Tumor Suppressor ; Genetic Therapy ; Humans* ; Liposomes ; Transfection*

To seek a practical and inexpensive method to preserve gonococcal cultures, a few methods were compared. The following methods kept the cultures alive for only a short period of time: skim milk at -20 degrees C; tryptic soy broth with 15% glycerol at -20 degrees C; cystine tryptic agar at 35 degrees C. Most of the test cultures survived for more than 4 weeks in the following media: one half strength dextrose starch agar; one half strength dextrose starch agar with ferric nitrate; one half strength dextrose starch agar with antimicrobic CNV. Dextrose starch agar could be substituted by GC medium base with a slight modification. It is concluded that preservation of gonococci in one half strength dextrose starch agar with CNV, which produces less frequent contamination, is a practical method to maintain cultures for teaching and quality control.
Agar ; Bacteriological Techniques ; Culture Media* ; Glucose ; Neisseria gonorrhoeae* ; Preservation, Biological/methods* ; Starch

The ambulatory electrocardiographic examinations were performed in 31 patients (mean age of 59.4+/-9.3 yrs : male 16 cases, female 15 cases) with ischemic heart disease to evaluate the clinical features of ST segment more than 1 mm persisting for 45 seconds or longer. The incidence of associated disease are angina pectoris 14 cases, acute myocardial infarction 3 cases, old myocardial infarction 7 cases, hypertension 19 cases, diabetes mellitus 5 cases, cerebrovascular disease 4 cases, aortic regurgitation 2 cases, ventricular arrhythmia 1 case and chronic renal faliure 1 case. 93.7% of 252 monitored episodes of transient myocardial ischemia were silent. The incidence and duration of transient myocardial ischemia were 8.1+/-6.7 episodes/day (7.6+/-6.5episodes/day for silent myocardial ischemia, 0.5+/-0.9 episodes/day for silent ischemia, 7.6+/-14.1mins/day for symptomatic ischemia). The heart rate at the onset of ST segment depression is higher in symptomatic episode than silent episode (94.6+/-19.7 vs 82.1+/-17.4/min,. p<0.05). But duration of ST segment depression is longer in silent episode than symptomatic episode(32.4+/-97.7 vs 14.8+/-10.2/min,. p<0.01). Maximal ST segment depression was similar between silent and symptomatic episode (1.61+/-0.65 mm, 1.97+/-0.84 mm, repectively). 55.5% of silent episodes occurred during sleep or resting state and 60% of symptomatic episodes occurred during strenuous effort, exercise or eating (p<0.01). Transient myocardial ischemia developed not more frequently in the morning probably because the 24 hour Holter electrocadiographic examination was performed during hospitalization in the majority of cases.
Angina Pectoris ; Aortic Valve Insufficiency ; Arrhythmias, Cardiac ; Depression ; Diabetes Mellitus ; Eating ; Electrocardiography ; Female ; Heart Rate ; Hospitalization ; Humans ; Hypertension ; Incidence ; Ischemia ; Male ; Myocardial Infarction ; Myocardial Ischemia*

The development of glomerular injury in patients with malignancy is considered as paraneoplastic syndrome. The most frequently observed renal lesions associated with malignancies are the membraneous glomerulonephritis on carcinomas and minimal change nephrotic syndrome on Hodgkin's disease. However, glomerular diseases on non-Hodgkin's lymphoma were only occasionally reported. Here we report a case of IgA nephropathy associated with non-Hodgkin's lymphoma. A 53-year-old woman who had complained of gross hematuria and fever was admitted to Wonju Christian Hospital. A urinalysis revealed 2+ proteinuria and red blood cells >30/HPF. A 24-hour urinary protein excretion was 379mg. She was diagnosed as IgA nephropathy on renal biopsy. Subsequently, biopsy of her enlarged neck node was performed for evaluation of fever of unknown origin and it revealed non-Hodgkin's lymphoma (Ki-1 positive anaplastic lymphoma null cell type). Combination chemotherapy was instituted with cyclophosphamide, adriamycin, vincristine and prednisone. After 3 cycles of chemotherapy, she showed no evidence of proteinuria and hematuria with clinical and radiological improvement of malignant lymphoma. Therefore we suggest of certain association between IgA nephropathy and non-Hodgkin's lymphoma by the observation of corresponding disease activity.
Biopsy ; Cyclophosphamide ; Doxorubicin ; Drug Therapy ; Drug Therapy, Combination ; Erythrocytes ; Female ; Fever ; Fever of Unknown Origin ; Gangwon-do ; Glomerulonephritis ; Glomerulonephritis, IGA* ; Hematuria ; Hodgkin Disease ; Humans ; Immunoglobulin A* ; Lymphocytes, Null ; Lymphoma ; Lymphoma, Non-Hodgkin* ; Middle Aged ; Neck ; Nephrosis, Lipoid ; Paraneoplastic Syndromes ; Prednisone ; Proteinuria ; Urinalysis ; Vincristine

Kerion celsi is an inflammatory type of tinea capitis. It is a deep, boggy swelling and is painful. Patchy hair loss and broken hairs, inflammation and scaling are characteristic. We report a case of kerion celsi caused by Trichophyton(T.) mentagrophytes in 4 year-old boy. He presented with 6 cm x 7 cm and 2cm x 2cm sized erythematous boggy masses with multiple pustules and crusts on the vertex. Cultures from a scalp lesion of the patient on Sabouraud dextrose agar media showed T. mentagrophytes. Treatment was done systemically with itraconazole and prednisolone for 14 weeks.
Agar ; Child, Preschool ; Glucose ; Hair ; Humans ; Inflammation ; Itraconazole ; Male ; Prednisolone ; Scalp ; Tinea Capitis* ; Trichophyton*