2.Invasion Suppressor Role of E-Cadherin in Epithelial Cancer Cell Lines.
Annals of Dermatology 1997;9(4):263-269
BACKGROUND: The generation of the invasiveness in transfromed cells represents an essential step of tumor progression. The primary cause of the scattering of the cells in invasive carcinoma is a loss of the integrity of the intercellular adherens junction often involving loss of a functional cell-cell adhesion molecule E-cadherin. Therefore, the perturbation of E-cadherin function causes diaggregation of tumor cells and may promote the invasion and metastases. OBJECTIVE: The reduction in E-cadherin activity seems to correlate with the infiltrative ability of tumor cells. The purpose of this study was to compare the E-cadherin expression among different cell lines which were normal to undifferentiated and to check the virtual relationaship between E-cadherin and invasiveness. METHODS: We used 5 cell lines, HaCaT, A431, C3, SiHa and HeLa cell. To check the expression patterns and amounts of E-cadherin in each cell line, immunofluorescence staining, Western blot anlysis and Northern blot analysis were done. An in vitro invasion assay using the collagen gel and MRC-5 fibroblast under the influence of HECD-1 antibody which block the E-cadherin function was done to measure the invasiveness of tumor cells. Collagenase activity in culture supernatants of each cell were analyzed by zymography. RESULTS: Immunofluorescence staining revealed a homogenously well preserved pattern in HaCat, A431, C3 cells. SiHa cells showed patch distribution but HeLa cells did not express the E-cadherin. Western blot analysis and Northern blot results largely corresponded with the immunofluorescence results. The in vitro invasion assay revealed invasion into the collagen matrix of the HeLa cells. When HECD-1 antibody was added to the medium, other cells showed partially disrupted stratification. The collagenolytic activity at 72 kDa sixe was detected in the HeLa cell line only. CONCLUSION: There is an inverse relationship between E-cadherin expression and tumor invasion. Therefore, through their regulation of cell adhesion and motility, cadherin plays a crucial role in the suppression of tumor invasion and metastasis.
Adherens Junctions
;
Blotting, Northern
;
Blotting, Western
;
Cadherins*
;
Cell Adhesion
;
Cell Line*
;
Collagen
;
Collagenases
;
Fibroblasts
;
Fluorescent Antibody Technique
;
HeLa Cells
;
Humans
;
In Vitro Techniques
;
Neoplasm Metastasis
3.A Case of Subungual Osteochondroma.
Jae Wang KIM ; Kwang Joong KIM ; Chong Joo LEE
Korean Journal of Dermatology 1998;36(5):906-909
Herein we report a case of subungual osteochondroma in a male patient. Although there have been several reports about cases of subungual exostosis in Korea, a case of subungual osteochondroma had not been reported until now. Osteochcndromas are clearly different disease entities from exastosis in that they have diagnostic hyaline cartilaginous caps instead of the fibrocartilages in subungual exostosis. Additioaally, subungual osteochondroma is a very rare type having an incidence rate of less than 1% among all osteochondromas. Our patient was an 18-year-old male with a solitary woody mass on his right great toe that was painfuL A surgical excisional biopsy following a nail plate avulsion showed the characteristic hyaline cattilaginous caps enclosing bony trabecula connected with the inferior cortical bone.
Adolescent
;
Biopsy
;
Exostoses
;
Humans
;
Hyalin
;
Incidence
;
Korea
;
Male
;
Osteochondroma*
;
Toes
4.Species and antimicrobial susceptibility of enterococcus isolated from clinical materials.
Hee Joo LEE ; Yunsop CHONG ; Oh Hun KWON
Korean Journal of Infectious Diseases 1992;24(2):115-120
No abstract available.
Enterococcus*
5.Species and antimicrobial susceptibility of enterococcus isolated from clinical materials.
Hee Joo LEE ; Yunsop CHONG ; Oh Hun KWON
Korean Journal of Infectious Diseases 1992;24(2):115-120
No abstract available.
Enterococcus*
6.Intratumoral Injection of 166Holmium-chitosan Complex to SmallRenal Cell Carcinoma: Preliminary Results.
Min Chong LEE ; Joo Eui HONG ; Su Yeon CHANG ; Jong Tae LEE ; Sung Joon HONG
Korean Journal of Urology 2000;41(3):449-453
No abstract available.
7.A Case of Bacteremia by Plesiomonas shigelloides.
Hyukmin LEE ; Kyungja WOO ; Kyungwon LEE ; Yunsop CHONG ; Joo Hang KIM
Korean Journal of Clinical Microbiology 2000;3(2):137-141
Plesiomonas shigelloides was isolated from blood culture of a 53-year-old man with fever, who had treatment history of gastrointestinal malignancy. The patient showed neither clinical features nor hematological finding which suggest bacteremia. Identification of the isolate was delayed because of its similar characteristics with Aeromonas spp. and other gram-negative bacilli. The isolate was misinterpreted as susceptible to ampicillin by the first disk diffusion test. It may not always easy to identify P. shigelloides by conventional tests and to determine its antimicrobial susceptibility accurately, as laboratorians rarely have experience with the organism and as the organism may show unusual inhibition pattern when tested by disk diffusion method or Etest.
Aeromonas
;
Ampicillin
;
Bacteremia*
;
Diffusion
;
Fever
;
Humans
;
Middle Aged
;
Plesiomonas*
8.A Case of Bacteremia by Plesiomonas shigelloides.
Hyukmin LEE ; Kyungja WOO ; Kyungwon LEE ; Yunsop CHONG ; Joo Hang KIM
Korean Journal of Clinical Microbiology 2000;3(2):137-141
Plesiomonas shigelloides was isolated from blood culture of a 53-year-old man with fever, who had treatment history of gastrointestinal malignancy. The patient showed neither clinical features nor hematological finding which suggest bacteremia. Identification of the isolate was delayed because of its similar characteristics with Aeromonas spp. and other gram-negative bacilli. The isolate was misinterpreted as susceptible to ampicillin by the first disk diffusion test. It may not always easy to identify P. shigelloides by conventional tests and to determine its antimicrobial susceptibility accurately, as laboratorians rarely have experience with the organism and as the organism may show unusual inhibition pattern when tested by disk diffusion method or Etest.
Aeromonas
;
Ampicillin
;
Bacteremia*
;
Diffusion
;
Fever
;
Humans
;
Middle Aged
;
Plesiomonas*
9.Cutaneous Cytomegalovirus Infection Presenting as Papules and Pustules in a Patient with Acquired Immunodeficiency Syndrome.
Heun Joo LEE ; Ji Hye PARK ; Chong Won CHOI ; Ga Young LEE ; Won Serk KIM
Korean Journal of Dermatology 2012;50(12):1089-1090
No abstract available.
Acquired Immunodeficiency Syndrome
;
Cytomegalovirus
;
Cytomegalovirus Infections
;
Humans
10.An Experimental Study of Development of Preretinal Membrane Following Intravitreal Hemorrhage.
Chong Hak JEON ; Ho Kyung LEE ; Joo Hwa LEE
Journal of the Korean Ophthalmological Society 1987;28(6):1219-1227
It has been known that proliferative vitreoretinopathy(PVR) can be developed by various intraocular diseases. Among them, the intravitreal hemorrhage would be accounted one of the causative disorders of PVR. An experimental study of the rabbit retina following intravitreal injection of auto blood was performed in order to investigate of development processes and nature of cellular alterations in PVR. The results were as follows: 1. Macrophages containing several dense bodies appeared near the inner limiting membrane of the retina and many floating red blood cells were present in the vitreous cavity on 3 days after intravitreal injection of blood. 2. One week after intravitreal injection of blood, the Miiller cells extended to the vitreous penetrating the inner limiting membrane. Many macrophages were observed between the Miiller cell processes. 3. Preretinal proliferative tissue were differentiated to the multilayered Miiller cell processes with the cellular characteristics containing glycogen granules, SER and junctional complexes in 2 weeks after intravitreal injection of blood. In these states macrophages between the Miiller cell processes had were completely disappeared. 4. It was considered that Miiller cells extend to the vitreous penetrating the inner limiting membrane in order to phagocytose the red blood cells in the vitreous which lead to processes resulting in formation of PVR.
Erythrocytes
;
Glycogen
;
Hemorrhage*
;
Intravitreal Injections
;
Macrophages
;
Membranes*
;
Retina