Purpose: To evaluate postoperative visual acuity, refractivestatus and rotational stability of toric intraocular lens (IOL)in correcting pre-existing corneal astigmatism.Methods: A total of 69 patients with topographic cornealastigmatism of 1.0 Diopter (D) and above who underwentcataract surgery between June 2015 and December 2016were included in this retrospective observational study. Allpreoperative toric IOL calculations were performed usingimmersion biometry. Appropriate formula to calculate toricIOL power was applied (SRK/T, Holladay 1 or Hoffer Qformula). All patients undergone similar uncomplicatedphacoemulsification with implantation of AcrySoft IQ SN6ATtoric IOL of different powers. Visual outcome, refractivestatus and axis of lens were evaluated at six weekspostoperatively. Ethical approval from the Ministry of HealthMedical Research Ethics Committee was obtained prior tocommencement of study.Results: The mean refractive astigmatism decreased from1.69 D ±1.10 (SD) to 0.81 D ± 0.40 (SD) at six weekspostoperatively. The mean postoperative sphericalequivalent was at -0.37 D ±0.64 (SD). Mean LogMAR foruncorrected and corrected distance visual acuity in sixweeks postoperative patients was at 0.29 ±0.16 (SD) and 0.12±0.12 (SD) respectively. Intraoperative to 6 weeks ofpostoperative comparison of IOL axis alignment showed lowlevels of rotation (mean 3.21 ±2.52 degrees).Conclusion: Cataract surgery with implantation of toric IOLwas stable and effective in improving pre-existing regularcorneal astigm

BACKGROUND AND OBJECTIVES: Cancer cell line is the basic material of various cancer research. Diverse cancer cell lines from various head and neck regions are needed for biologic research of head and neck cancer. However, cell lines derived from head and neck cancer are not common. Recently, we have established and characterized a novel human squamous carcinoma cell line, PNUH-12, from the hypopharynx. MATERIALS AND METHODS:Among trials of twenty cases of head and neck cancer, we established only one specimen succeeded culture passage over 50. We characterized the cell line as follows: growth pattern and curve, morphology using phase contrast microscope and transmission electromicroscope, chromosomal anaysis, flow cytometric analysis, tumorigenecity by xenograft of cell line into nude mouse and morphological comparison, expression of cytokeratin, epithelial membrane antigen and vimentin, and P 53 mutation and its sequencing. RESULTS: PNUH-12 showed typical growth pattern of cancer cell line, representative morphological characteristics of squamous epithelial cell origin, multiple numerical and structural clonal abnormalities of chromosome, aneuploidy pattern of flow cytometry, and strong expression of cytokeratin. The formed tumor of nude mouse showed the identical histopathological phenotype (squamous cell carcinoma) of the original tumor of patient and similar morphology of PNUH-12. There was one point mutation of 78th base, C to G, in exon 7 of P53 gene. CONCLUSION: PNUH-12 can be a good control material and successufully bestowed to researchers for study of biology in head and neck cancer. There still needed more head and neck cancer cell lines from various regions and diverse cell types in future.
Aneuploidy ; Animals ; Biology ; Carcinoma, Squamous Cell* ; Cell Line* ; Epithelial Cells ; Exons ; Flow Cytometry ; Genes, p53 ; Head ; Head and Neck Neoplasms ; Heterografts ; Humans* ; Hypopharynx* ; Keratins ; Mice ; Mice, Nude ; Mucin-1 ; Neck ; Phenotype ; Point Mutation ; Vimentin