A surveillance system for cholera control was conducted in Hai Phong from February to December 1998 in Ngo Quyen and Thuy Nguyen districs. The isolation of V.cholerae from stool samples of acute diarrhoea on one fixed day per week was the index study. The result of the study contributed to improve the effectiveness of the surveillance system.
Cholera ; epidemiology

The results of cholera surveillance from 1986-2000 showed that the incidence of the disease has dramatically decreased from 9.37 per 100.000 population in 1986 to 0.14 per 100.000 population in 2000. The disease has occurred all year round. However, the highest incidence was in January. February and the lowest incidence was in July, August. Cholera has mainly been recorded in provinces of central and Southern regions. Causative agent was Vibrio cholerae El Tor serotype Ogawa. The disease has spread through ingestion of seafood shrimp and fish sauces, water contaminated with Vibrio cholerae.
Cholera ; epidemiology

To confirm whether cholera epidemic in Hai Phong city is current or invasive, the stool samples from old cholera cases, healthy people, acute diarrhea cases as well as samples from environment such as oyster, fish, mussel etc. were collected for isolating V.cholerae 01. Not any of them was positive with V.cholera 01. It means that there was no evidence of current cholera epidemic in Hai Phong. The new cholera surveillance system was proposed for Hai Phong city.
Cholera ; epidemiology

Reviewing cholera epidemics in Thua Thien-Hue province yielded that people at all age groups had caught cholera, 36% of all these cholera cases were in less than 15 years old group. In 2003, there was a transform from type V.E. OGAWA to V.E. INABA. The C-degree dehydration has been increased and made 50% among cholera patients, statistically higher than the rates of the years 2003, 1992 and 1993. This bacterium was still sensitive to common antibiotics shown on antibiograms. However ampicilline has been at risk of drug resistance. Therefore, it is necessary to carefully choose antibiotics for treatment and chemo-prophylaxis for people.
Cholera ; Epidemiologic Studies ; Epidemiology

Recently, two successive epidemics of cholera were observed in Korea. The first one started in Suhchun-Goon of Choong-Chung-Namdo in August 1969, and the 2nd in Changyoung-Goon of Kyung Sang-Namdo in August 1970. With stool specimens collected from patients in Suhchun, Ko-Chang, Seoul, Inchun, Youngkwang, Chang-hang and Wooljin were epidemic areas in l969, and from patients in Chang-Young, Pusan, Taegu, and Seoul which were epidemic areas in l970, studies were carried out in 1) the isolation and identification of cholera vibrio strains 2) the differentiation of E1 Tor vibrio from classic cholera strains 3) the liberation test of Kappa-type phages and 4) El Tor phage typing. Five strains, which were isolated in the epidemic area of the Philippines in l969 were included for a comparative study. The results are summarized as follows. 1) The epidemic strains of 1969 were identified as Vibrio cholerae, Celebes type El Tor and those of 1970 epidemic as Vibrio cholerae, biotype El Tor, El Tor phage type IV. 2) Korean strains and Philippine strains of 1969 epidemic appeared to be identical in biochemical and serological tests and phage susceptibility tests.
Cholera/epidemiology ; Cholera/microbiology* ; Human ; Korea ; Philippines ; Vibrio/isolation & purification*

Cholera ; epidemiology ; Disease Outbreaks ; Humans ; Zimbabwe ; epidemiology

China ; epidemiology ; Cholera ; epidemiology ; Disease Outbreaks ; Humans

OBJECTIVETo understand the epidemic condition, distribution and biological characteristics of non-O1/non-O139 Vibrio cholerae from 2001 to 2009 in Haizhu District, to provide a scientific basis for the prevention and control of acute diarrhea.

METHODSReferring to the detecting method written in "Cholera control handbook" in the fifth edition, 764 specimens from outside environment (including the water in the Pearl River, drinking water, water for breeding fish, aquatic products and delicatessen foods), 189 specimens of healthy population and 3398 intestinal samples of patients with diarrhea, summing up to 4351 specimens for non-O1/non-O139 Vibrio cholerae test.

RESULTS4,351 specimens were detected of 101 strains of non O1/non O139 Vibrio cholerae, the total detection rate was 2.32%; 66 strains were identified by serotyping and grouped into 26 different serotypes, the typing rate was 65.3%. The strains VBO9, VBO38 and VBO76 were the dominant bacteria.Nine strains of the same type of non-O1/non-O139 Vibrio cholerae were found from external environments also from patients with diarrhea, suggesting that there might be a correlation between the two.

CONCLUSIONNon-O1/non-O139 Vibrio cholerae have diversified serotypes, causing certain infection rate among the population in this region. These bacteria exist extensively in external environment and they are the potential hazard to the citizens.

China ; epidemiology ; Cholera ; epidemiology ; microbiology ; Humans ; Serotyping ; Vibrio cholerae ; classification ; isolation & purification

OBJECTIVETo determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.

METHODSChromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.

RESULTS39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.

CONCLUSIONThe closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.

China ; epidemiology ; Cholera ; epidemiology ; microbiology ; Electrophoresis, Gel, Pulsed-Field ; methods ; Humans ; Phylogeny ; Vibrio cholerae ; classification ; genetics

Animals ; China ; epidemiology ; Cholera ; epidemiology ; prevention & control ; Environmental Monitoring ; Vibrio cholerae ; genetics ; isolation & purification