OBJECTIVE: The extraction method for separating buprenorphine from urine was described. METHODS: Buprenorphine was extracted with chloroform at pH7 or with SPE (extracted by organic support 401 at pH10.8, then eluted with chloroform), finally determined by GC-NPD. RESULTS: The extracted yields of the analyte in specimens were 86.6% by solution-phase extraction and 83.0% by solid-phase extraction (SPE) respectively. CONCLUSION These two methods are simple and accurate for separating buprenorphine from urine.
Buprenorphine/urine* ; Chlorpropham/chemistry* ; Chromatography, Gas/methods* ; Chromatography, Liquid/methods* ; Humans ; Hydrogen-Ion Concentration ; Reproducibility of Results ; Solid Phase Extraction/methods* ; Solvents/chemistry*