2.Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection.
Shan Shan LIU ; ; Jun CHU ; Qiang ZHANG ; Wei SUN ; Tian Yuan ZHANG ; Cheng HE
Biomedical and Environmental Sciences 2016;29(5):315-322
OBJECTIVEChlamydia psittaci is an avian respiratory pathogen and zoonotic agent. The wide prevalence of C. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies against C. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen.
METHODSThe antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens.
RESULTSThe sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively.
CONCLUSIONThese data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance of C. psittaci infection.
Animals ; Bacterial Proteins ; analysis ; Chickens ; Chlamydophila psittaci ; genetics ; immunology ; isolation & purification ; Enzyme-Linked Immunosorbent Assay ; veterinary ; Membrane Proteins ; analysis ; Poultry Diseases ; diagnosis ; microbiology ; Psittacosis ; diagnosis ; microbiology ; veterinary ; Sensitivity and Specificity
3.Relationship between primary ocular adnexal mucosa-associated lymphoid tissue lymphoma and eye infection.
Dan-dan ZHANG ; Hong-gang LIU ; Hai-yan LI ; Zi-fen GAO ; Xiao-ge ZHOU ; Ha-si JIN ; Li-na DONG ; Jing ZHANG ; Li-ping GONG
Chinese Journal of Pathology 2009;38(8):513-518
OBJECTIVETo study the role of pathogenic microorganisms commonly associated with chronic eye disease, including Chlamydia psittaci, Chlamydia trachomatis, Chlamydia pneumoniae, herpes simplex virus (HSV) type 1 and type 2, and adenovirus type 8 and type 19, in the development of primary ocular adnexal mucosa-associated lymphoid tissue (MALT) lymphoma in Chinese patients.
METHODSSixty-eight archival cases of primary ocular adnexal lymphoproliferative lesions, including 38 cases of MALT lymphoma, 3 cases of non-MALT lymphoma and 27 cases of chronic inflammation, were enrolled into the study. DNA was extracted from the paraffin-embedded tissue samples. The presence of DNA of C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 were analyzed by multiplex touchdown enzyme time-release polymerase chain reaction (TETR-PCR).
RESULTSAll of the specimens yielded PCR products of over 100 base pairs and were thus suitable for TETR-PCR screening of infectious agents. The prevalence of DNA of C. psittaci, C. trachomatis and adenovirus type 19 were 0 in MALT lymphoma, non-MALT lymphoma and chronic inflammation. There were 2 cases positive for C. pneumoniae DNA, amongst the 38 cases of MALT lymphoma studied (5.3%, 2/38). HSV type 1, HSV type 2 and adenovirus type 8 DNA was found in each of the 3 patients with chronic inflammation.
CONCLUSIONThe study indicates that C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 probably play little role in the pathogenesis of ocular adnexal MALT lymphoma in Chinese patients.
Adenovirus Infections, Human ; virology ; Adenoviruses, Human ; genetics ; isolation & purification ; Chlamydia Infections ; microbiology ; Chlamydia trachomatis ; genetics ; isolation & purification ; Chlamydophila Infections ; microbiology ; Chlamydophila pneumoniae ; genetics ; isolation & purification ; Chlamydophila psittaci ; genetics ; isolation & purification ; DNA, Bacterial ; analysis ; DNA, Viral ; analysis ; Eye Infections ; microbiology ; virology ; Eye Neoplasms ; microbiology ; virology ; Herpes Simplex ; virology ; Herpesvirus 1, Human ; genetics ; isolation & purification ; Herpesvirus 2, Human ; genetics ; isolation & purification ; Humans ; Lymphoma, B-Cell, Marginal Zone ; microbiology ; virology ; Psittacosis ; microbiology