Objective To construct the fusion gene of membrane type matrix metalloproteinase1 humanized tandem affinity purification (MT1 MMP hTAP) and to evaluate the expression of its protein complexes isolated and purified using tandem affinity purification (TAP) system. Methods The MT1 MMP hTAP protein complexes were isolated and purified using the IgG agarose beads and calmodulin sepharose beads affinity chromatography according to the molecular and biological features of TAP system. The expression levels of its protein complexes were determined by PAP immunostaining and Western blotting. Results PAP antibody immunostaining showed that MT1 MMP hTAP protein complexes (dark blue) were expressed in chick fibroblast cells. Western blot analysis of its protein complexes using MT1 MMP specific antibody showed a prominent band (68?10 3) of mobility consistent with fully processed MT1 MMP hTAP after being cleaved by the TEV proteinase. Conclusion The construction of TAP system in chick fibroblast cells and expression of MT1 MMP hTAP protein complexes will be helpful for the studies of the MT1 MMP hTAP interaction proteins in the human tumor cells.

America medical education system has more important effect on our long-education program.Based on the analysis of the characters in medical education between China and America,several suggestions about obstetric and gynecological teaching have been brought up to accommodate the new tendency,as well as the culture of specialists in the future.

Objective To develop a mouse model of premature ovarian failure (POF) by chemical treatment or radiotreatment. Methods Eighty Kunming mice were divided into four groups, including chemical treatment group that were administered Cyclophosphamide and Busulfan, radiotreatment group receiving 60Co ? ray irradiation, control group and castrated group. To evaluate the animal model, vaginal smears were collected daily for estrous cycle determination, and serum hormone levels and histological changes in ovaries were examined. Results Estrous cycles became irregular after chemical treatment or radiotreatment (P0.05). Compared to control group, ovaries from chemical treatment and radiotreatment groups showed ovarian atrophy and interstitial fibrosis. The number of growing follicles and non-atretic primordial follicles was notably reduced (P

BACKGROUND:Bone marrow mesenchymal stem cells(BM-MSCs)have been shown to possess the potential to differentiate into oocytes.However,immune rejection and a limited number of donors of BM-MSCs constrain the applications of BM-MSCs.Several studies have demonstrated that human umbilical cord matrix stem cells(UC-MSCs)also have an intrinsic ability to differentiate into oocyte-like cells in vitro.OBJECTIVE:To establish the method for UC-MSCs culture and to investigate the in vitro differentiation potential of UC-MSCs towards germ cells.METHODS:Umbilical cord from full-term normal deliveries was obtained in sterile condition.Collagenase I-digested cells were cultured in DMEM.The immunophenotype of cells was determined by flow cytometry.Lipoblasts,osteoblasts and chondroblasts were induced in different condition cultures.The expression of germ cells specific marker in UC-MSCs was determined by reverse transcdption-polymerase chain reaction.Follicular fluid was employed to induce UC-MSCs differentiation into germ cells.RESULTS AND CONCLUSION:Spindle-like umbilical cord cells were shown and cells in culture were extended to more than 10passages.BM-MSCs-like immunophenotypes were shown:CD29,CD44,CD73(SH3),CD90 and CD105(SH2)were positive;SSEA-4 was weakly positive;CD31,CD34,CD45 and HLA-DR were negative.After UC-MSCs were induced in different condition cultures,lipid droplet-,bone tubercle-,and cartilage tubercle-like structures emerged and the mRNA expressions of specific gene of fat,bone and cartilage were observed.Germ cells markers,OCT4,Stella,Ifitm3,were expressed in UC-MSCs.After induced by 5%,10% or 20% follicular fluid,cells aggregated and oocyte-like structures were observed.Human UC-MSCs could be cultured and amplificated in vitro.UC-MSCs showed immunophenotypes similar to BM-MSCs.UC-MSCs had the potential to differentiate into lipoblasts,osteoblasts,and chondroblasts.Oocyte-like structure was induced in vitro from UC-MSCs with germ cells specific marker.These findings suggest that UC-NSCs have the potential to differentiate into germ cells.