The influence of Cibotium barometz (L.) J. Sm. and its processed samples on thrombin-induced platelet aggregation in rabbits was studied. The results showed that all differently processed sam-ples tested could inhibit platelet aggregation, with activities in the decreasing order of Rhizoma Cibotiiroasted in stirring sand＞steamed after being salted＞steamed after steeped in wine＞simply steamed＞theunprocessed crude Rhizoma Cibotii.

Effects of saponin of Panax pseudo-ginseng var. notoginseng (Burk.) Hoo et Tseng(PnS) on neutrophil (Neu) count, protein and malondialdehyde (MDA) contents in the exudate of rat a-cute air pouch synovitis model induced by sc injection of 25 mg/kg carrageenan were investigated by Lowryand test tube dilution methods and thiobarbituric acid spectrophotometry. Its effects on the intracellularcAMP content in and release of O2 from Neu were further assessed by radioimmunoassay and cytochromeC reduction methods respectively. The results showed that PnS 60,120,240 mg/kg ip reduced the migra-tion of Neu and exudation of protein, lowered the content of MDA and inhibited the release of O2- fromNeu; but elevated the cAMP content of Neu in a dose-dependent manner. These results revealed that Pnshas an obvious anti-inflammatory effect and its primary action was related to the increase of cAMP contentin Neu, followed by inhibition of the free radical production.

The optimum conditions for the processing of Sparganium stoloniferum Buch-Ham. withvinegar were studied by L9(3)4 orthogonal experimental design, as guided by decrease of writhing rate inanalgesic test and anticoagulant activity. The results showed that the best processing conditions were stirfrying of the raw S. stoloniferum with the addition of 25 kg of vinegar per 100 kg of crude drug at 130℃for 10 min.

Anthranilic acid in the leaves of Isatidis indigotica Fort. was determined by first orderderivative UV spectrophotometry. The average recovery was 96.66％ and the coefficient of deviation was1.09％. The method showed no significant difference as compared with high performance capillary elec-trophoresis at 95％ confidence level. The method is simple, rapid and accurate.

Epicatechin content in Uncaria macrophylla Wall. was determined by RP-HPLC. As aresult, the epicatechin contents were 0.38％ in the hook and stem, and 0. 820％ in the leaf. Thus it seemedto be more worthwhile to produce epicatechin from the leaf rather than from the hook and stem.

The content of total alkaloid in the seed of Peganum harmala L. was determined by bro-mophenol blue colorimetry. It could be considered that this is a specific, quick and simple method for thedetermination of total alkaloid in the seed of P. harmala.

A simple method for the isolation and purification of ginkgolide A, B and bilobalide(ginkgo terpene trialctone) was developed. A commercially available extract of leaves of Ginkgo biloba L.containing ＞6％ ginkgo terpene trilactone was used as the raw material. After partition in EtOAc, the en-riched extract was separated to give individual terpenes by preparative liquid chromatography. GinkgolideA, B and bilobalide could be isolated with high purity by recrystallizing in MeOH-H2O.

Three triterpenoid saponins were isolated from 90％ ethanolic extract of the seeds of Aes-culus turbinata Bl.. Their structures were identified on the basis of spectral data as escin IVc:22α-O-tigloyl-28-O-acetylprotoaescigenin 33-O-[β-D-glucopyranosyl (1→2)][β-D-glucopyranosyl (1→4)]-β-D-glucopyranosiduronic acid (Ⅰ), isoescin Ia: 21β-O-tigloyl-28-O-acetylprotoaescigenin-3β-O-[β-D-glucopy-ranosyl (1→2)][β-D-glucopyranosyl (1→4)]-β-D-glucopyranosiduronic acid (Ⅳ), and isoescin Ib:21β-O-angeloyl-28-O-acetylprotoaescigenin-3β-O-[β-D-glucopyranosyl (1→2)][β-D-glucopyr anosyl (1→4)]-β-D-glucopyranosiduronic acid (Ⅴ). Escin IVc and isoescins Ia, Ib were obtained for the first time from thisplant. Escins Ia, Ib, IVc and isoescins Ia, Ib had inhibitory effect against recombinant HIV-1 protease atconcentration of 100 μmol/L in vitro.

Composition of the polysaccharide from Bupleurum scorzonerifolium Willd. was studiedby high resolution capillary gas chromatography after hydrolysis and followed by acetylation and esterifica-tion of the hydrolysate. The results showed that it is composed of arabinose, ribose, D-xylose, D-man-nose, D-glucose and D-galactose, with a molar ratio of 0. 106 : 2. 652 : 4. 070: 0. 519 : 0. 930 : 2. 518.

In continuation of our investigation, a new diterpene, rabdonervosin C (Ⅰ), was isolat-ed from the leaves and stems of Rabdosia nervosa (Hemsl.) C. Y. Wu et H. W. Li. Its structure wasidentified as lα, 15β, -dihydroxy-6β-ethoxy-6, 7-B-seco-ent-kaur-16-en-6, 20-epoxy-7, 20-δ-olide on thebasis of spectroscopic (MS, IR, 1HNMR, 13CNMR, DEPT and 2D-NMR) data and in comparison with theknown rabdonervosin A(Ⅲ) and B (Ⅱ).