AIM To analyze the dynamic changes of five constituents in Ligustri lucidi Fructus at five picking time (August,September,October,November,December).METHODS The HPLC analysis of Ligustri lucidi Fructus ethanol extract was performed on a 25 ℃ thermostatic Aglient Zorbax SB-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 224 nm.RESULTS Salidroside,tyrosol,luteolin-7-O-glucoside,ligustroflavone and specnuezhenide showed good linear relationships within their own ranges (r ＞0.999 0),whose average recoveries were 99.56％-100.30％ with the RSDs of 0.89％-1.23％.The contents of various constituents (except for tyrosol) were the highest in samples picked up in September,followed by those picked up in October.CONCLUSION The suitable picking time of Ligustri lucidi Fructus is September and October.

AIM To determine the contents of chemical consituents in Lycii Fructus from Qaidam Basin.METHODS Spectrophotometry was adopted in the content determination of polysaccharides,total flavonoids and carotenoid.HPLC was applied to the content determination of betaine and scopoletin.Kjeldahl method was used for the content determination of protein.Then principal component analysis was performed.RESULTS The contents of carotenoid,betaine and scopoletin in samples from six growing areas showed obvious differences (P ＜ 0.05),while those of polysaccharides,total flavonoids and protein exhibited no obvious differences (P ＞ 0.05).The contents of various constituents in samples at three picking time also had no obvious differences (P ＞ 0.05).The comprehensive score of principal components of samples from Delingha City was the highest,followed by that from Ulan County.CONCLUSION The quality of Lycii Fructus from Qaidam Basin from Delingha City is the best.

Utilizing the classical method——destruction test of thermostatic heat acceleration and using the residual content of total anthraquinone of Radix et Rhizoma Rhei as an index, the shelf life of Maren Soft Capsules was predicted as 2.13 years when the data were computed and dealt according to Arrhenius eguation

AIM: To investigate the epidemiological characteristic and causative factors of anaphylactic shock caused by Shuanghuanglian Injection(Flos Lonicerae Japonicae,Radix Scutellariae,Fructus forsythiae). METHODS: Domestic medicine periodicals were searched between 1978 and 2005,and 72 cases with anaphylactic shock caused by Shuanghuanglian Injection were analysed by literature metrologic method. RESULTS: There were large proportion of young sufferers from age 18 to 44 in the case reports,and male 52.11%,female 47.89%,the primary affections were mainly upper respiratory tract infection,the occurrence of ADRs was commonly within 30 mins after injection,seven patients died of the terrible anaphylactic shock. CONCLUSION: Shuanghuanglian Injection may induce dangerous,even fatal anaphylactic shock,its mechanism includes many factors,such as patient's physical constitution.

AIM:Antioxidants act mainly through two routes:induction of antioxidant enzymes(enzymatic)or direct reaction with reactive oxygen species(ROS)(non-enzymatic),but the one taken by the extract of Ginkgo biloba(EGb)remains to be investigated.In present study,EGb was tested for both of the antioxidant routes in vitro.METHODS:The induction of EGb on two antioxidant enzymes,glutamate cysteine ligase catalytic subunit (GCLC)and glutathione-S-transferage subunit-P1(GST-P1),in cell lines wag detected by Western-blot.The effects of EGb on superoxide anion radical(O2·-),hydroxyl radical(OH·),rat erythrocyte hemolysis and lipid peroxidation of rat liver homogenate were determined by activity methods respectively.RESULTS:EGb was dem onstrated significantly to induce the two antioxidant enzymes(GCLC and GST-P1),directly scavenge superoxide anion radical(O2·-),hydroxyl radical(OH·)and inhibit rat erythroeyte hemolysis and lipid peroxidation of rat liver homogenate.CONCLUSION:The results strongly support the notion that EGb plays dual antioxidant roles: induction of antioxidant enzymes and direct reaction with ROS.

AIM: This study aimed at investigating the effects and mechanism of total alkaloids from Rhizoma Coptidis (TA) on ethanol-induced gastric mucosal injury in rats. METHODS: The experimental gastric damages were established by intragastric ethanol, and the protective effects of TA were evaluated by calculating lesion indices contents and superoxide dismutase (SOD) activity from rat gastric mucosa were measured to explore the interrelation between therapeutic effects of TA and these factors. The expressions of neuronal nitrogen monoxide synthase (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS)from ethanol-damaged gastric mucosa in rats were analysised using immunohistochemical method. RESULTS: TA significantly inhibited the gastric injury induced by ethanol ,in dose-dependent manner,and the effect of TA was superior to that of Berberine (Ber). TA obviously antric mucosa. TA significantly suppressed ethanol-induced decreasing nNOS and eNOS expression and elevation of iNOS expression in rat gastric mucosa. CONCLUSION: Rhizoma Coptidis is a potent candidate in therapeutic drugs of human alcohol-induced gastric injury. Its anti-injury effects involve in Ber and other ingredients of TA. The protective mechanisms of TA involve in inhibiting generation of oxygen-derived free radical, accelerating scavenging of free radicals, relieving lipid peroxidation, and maintaining NO content in normal level by inhibiting decreasing of nNOS and eNOS expression and elevation of iNOS expression.