1.Study on the quality standard for Liyan granule
Chinese Pharmaceutical Journal 2001;(2):125-127
OBJECTIVE To establish a quality standard for Liyan granule.METHODS Honeysukle flower in the granule was identified by TLC.The developing agent was the upper solvent of butyl acetate formil acid-water(7∶2.5∶2.5).The dual wavelength spectrophotometry was applied for the determination of chlorogenic acid in granule.And the acute toxicity test was accomplished.RESULT The TLC for the identification of Honeysule flower in the granule was accurate.The uv for chlorogenic acid was reliable.The linear range of chlorogenic acid was 0.46~46.00 μg*mL-1(r=0.9992),and the recovery was 97.33%,RSD=1.49%.The acute toxicity test showed that the maximal tolerable dose in rats was equivalent to 850 times of the clinical dosage in human.CONCLUSION This method could be used as the quality control during the production of Liyan granule.
2.Determination of Pd-Ⅰa and Pd-Ⅱ in Peucedanum praeruptorum by GC
Qin XU ; Buming LIU ; Zhengxing ZHANG
Chinese Pharmaceutical Journal 2001;(2):122-125
OBJECTIVE To establish a GC method for the determination of the contents of Pd-Ⅰa and Pd-Ⅱ in roots of Peucedanum Praeruptorum.METHODS The determination was performed on a silica capillary SE-30(0.25 μm 0.22 mm×25 m)column by FID detector;with n-C25H52 as the internal standard.RESULTS The standard curves of Pd-Ⅰa and Pd-Ⅱ were linear over the range from 0.175 μg to 1.919 μg(r=0.9995) and 0.086 μg to 0.950 μg (r=0.9996),respectively.The average recoveries were (98.28±2.07)% for Pd-Ⅰa and (99.58±2.44)% for Pd-Ⅱ.Compared with HPLC,there was no significant difference in the results between GC and HPLC.CONCLUSION The method appeared to be simple,sensitive,rapid and accurate and can be used for the quality control of Chinese medicine Qianhu.
3.Protective effects of tiopronin on liver in antitubercucar treatment
Chinese Pharmaceutical Journal 2001;(2):129-130
OBJECTIVE To study the protective effects of Tiopronin in antitubercular treatment.METHODS The patients in the therapeutic group took Tioprooin orally and had administration of antitubercular drugs,while those in the control group used antitubercular drugs only.The changes of their ALT and TBIL were observed druing the course.RESULTS The abnormalities of ALT and abnormalities of ALT and TBIL at the same time in the thenapeutic group were obviously lower than those in the control group.CONCLUSION Tiopronin may prevent liver injury caused by antitubercular drugs.
4.Clinical study on meropenem and imipenem/cilastatin in treatment of respiratory tract infections
Yuming SONG ; Jianjun ZHAO ; Lu SUN
Chinese Pharmaceutical Journal 2001;(2):128-129
OBJECTIVE To evaluate the availability and safety of a new carbapenem meropenem.METHODS The randomized study was conducted in 60 patients with respiratory tract infections.They were divided into two groups:meropenem or imipenem/cilastatin.RESULTS The cure rate,effective rate and bacterial clearance rate for meropenem were 80.0%,93.3%,92.3%,respectively and for those of imipenem/cilastatin were 76.7%,90.0% and 91.3%,respectively.The rate of adverse reactions for both meropenem/cilastatin was 3.3%.CONCLUSION Meropenem appeared to be a highly effective and safe agent in treatment of respiratory tract infections.Its efficacy and safety are similar to that of imipenem/cilastatin.
5.High-performance liquid chromatographic determination of floxuridine in human serum
Peng WANG ; Zhu ZHU ; Qiang FU ; Min YE ; Dakui LI
Chinese Pharmaceutical Journal 2001;(2):118-120
OBJECTIVE To develop an HPLC method for the determination of floxuridine in human serum.METHODS With metronidazole as the internal standard,200 μL of serum was extracted by n-propyl alcohol/methyl t-butyl ether in a two-step extraction.The organic layer was evaporated under nitrogen stream and the residual was reconstituted with the mobile plase.A Shim-Pack CLC-ODS column was selected and the mobile phase was consisted of acetonitrile-phosphate buffer-water (75∶100∶900) at a flow rate of 0.6 mL*min-1.The detection wavelength was 268 nm.RESULTS A linearitywas obtained from 0.005 to 0.5 mg*L-1 of floxuridine in serum with a good correlation coefficient (r=0.9999,n=8).The intra-run and inter-run coefficients of variation were less than 4.09%.The mean recoveries were 103.00%,107.00% and 100.88% for the low,middle and high concentrations of check samples,respectively.The limit of detection was 0.001 mg*L-1.CONCLUSION The method was sensitive,specific and simple.It is suitable for clinical pharmacokinetic study.
6.Study on the determination of metronidazole and amoxicillin in Bijiaxilin tablet by HPLC
Chinese Pharmaceutical Journal 2001;(2):115-118
OBJECTIVE In order to control the quality of Bijiaxilin tablet, a method for the determination of metronidazole and amoxicillin was developed.METHOD A HPLC procedure was established.A 6.0 mm×150 mm Shim-Pack CLC-ODS column with a mobile phase consisting of pH6.5,0.02 mol*L-1 phosphate buffer-acetonitrile(90∶10) was used.A 10 μL portion of the sample solution was chromatographed and detected at 272 nm.The effects of the types of the mobile phase on the retention behaviour of metronidazole,amoxicillin and the other components in the samples were investigated.The effect of the solvent used for sample preparation on the stability of metronidazole and amoxicillin was also studied.RESULTS Metronidazole,amoxicillin and the other components in the samples were separated completely.The recoveries were 99.88% for metronidazole and 99.96% for amoxicillin with RSD less than 1.7%.CONCLUSION The proposed method is selective,simple,accurate and precise.
7.Pharmacokinetics of levofloxacin-methane sulfonic acid in patients with pulmonary tuberculosis
Wei LIU ; Duanhao FENG ; Weiai LU ; Hongmin LI ; Wei WANG ; Xianjie ZHANG ; Guizhi WU ; Ling SONG
Chinese Pharmaceutical Journal 2001;(2):113-114
OBJECTIVE To study the pharmacokinetics of levofloxacin-methane sulfonic acid (LF-MSA)in 8 patients with pulmonary tuberculosis after administration 400 mg orally.METHODS The concentrations of LF-MSA in serum were measured by HPLC.The pharmacokinetic paramters were obtained using 3P97 program.RESULTS The results showed that a one-compartment model with 1 st order absorption were fitted for LF-MSA.The average values of tmax was 1.22 h.The concentrations of LF-SA in serum was (4.52±0.72) mg*L-1.The mininal concentrations of LF-AS at 24 hr after a single dose was (0.52±0.26) mg*L-1.The average area under the curve was (48.35±5.38) mg*h*L-1.The elimination half-life was (6.49±1.70) h.The apparent volume of distribution was (77.48±8.33)L.CONCLUSION According to our study,it was suggested that 400 mg twice daily may be given in order to maintain the concentrations of LF-SA in serum around 24 hours above the minimal concentrations of bactericide.
8.Determination of paeoniflorin in Fufang Biejia Ruangan Tablet by TLC scanning
Mao YI ; Lingyun NIE ; Yun LIU ; Ke SONG
Chinese Pharmaceutical Journal 2001;(3):196-198
OBJECTIVE To establish a method for the determination of paeoniflorin in Fufang Biejia Ruangan tablet.METHODS The tablets were extracted with 50% ethanol.The sample solution was applied on a silica gel G-CMC plate and developed with chloroform-methanol-glacial acetic acid (85∶13∶2) and was sprayed with sulfuric acid containing 5% vanillin.The separated spot was determined by scanning with CS-930 at λS=575 nm,λR=700 nm.RESULTS The average recovery was 97.60% and the RSD was 3.67%.CONCLUSION The results showed that this method is sensitive,specific,accurate and reproducible.
9.A dual-wavelength spectrophotometry of serum theophylline with concomitant use of ofloxacin
Xian HUANG ; Lingping FANG ; Jianhua XU ; Fenghui YOU ; Xiaofang ZENG
Chinese Pharmaceutical Journal 2001;(3):194-196
OBJECTIVE To develop a method for the determination of theophylline in serum with concomitant use of ofloxacin.METHODS Theophylline was serum was extracted with chloroform-hexane(7∶3,v/v) after some ammonium sulfate was added.It was re-extracted with NaOH solution (0.1 mol*L-1)and detected with a UV spectrophotometer at 275 nm and 299.5 nm respeitively.RESULTS The method was linear over the range of 5.0~40.0 mg*L-1(r=0.9997,n=7).The average recovery of methodology was 100.3%(RSD=1.2%),and the extraction recovery from serum was 87.7%(RSD=4.6%).CONCLUSION This practical method for the determination of serum theophylline can eliminate the interference of ofloxacin and other antibiotics.
10.Study on the preparation of piroxicam films
Chinese Pharmaceutical Journal 2001;(3):172-173
OBJECTIVE To prepare the piroxicam films and study its characteristics of transdermal delivery.METHODS Different high polymers were employed to prepare piroxicam films.Rat abdomen skin and modified Franz diffusion cells were used in the drug penetration tests and release tests.The percutaneous penetration rate was investigated.RESULTS The release rate of piroxicam films was 64.31 μg*cm-2*h-1.The in vitro permeation rate was 14.86 μg*cm-2*h-1.The percutaneous penetration rate was 11.94 μg*cm-2*h-1.CONCLUSION Piroxicam films was developed.