Animals ; Estrogens ; metabolism ; Gene Expression Regulation ; Humans ; Osteoarthritis ; complications ; genetics ; metabolism ; Pain ; etiology ; Pain Measurement ; Temporomandibular Joint ; pathology ; Temporomandibular Joint Disorders ; complications ; genetics ; metabolism ; Wnt Signaling Pathway

Arthritis, Juvenile ; diagnosis ; therapy ; Humans ; Magnetic Resonance Imaging ; Orthodontics, Corrective ; methods ; Temporomandibular Joint Disc ; diagnostic imaging ; Temporomandibular Joint Disorders ; diagnosis ; diagnostic imaging ; therapy ; Tomography, X-Ray Computed

OBJECTIVETo study the prevalence of temporomandibular disorders (TMD) in 352 aged edentulous individuals in Beijing area and to analyze the correlative risk factors.

METHODSThree hundred and fifty-two aged edentulous subjects were included in the study (198 males and 154 females). The adopted questionnaire was designed according to Helkimo index, and the subjects were examined by the same examiner who would record every result as well.

RESULTSThe prevalence of TMD's clinical positive signs in the edentulous subjects was 43.2% (152/352), among which temporomandibular joint (TMJ) noise [34.1% (120/352)] was the highest prevalent sign while TMJ pain on movemert was the lowest. The prevalence of mandibular movement deviation [18.2% (64/352)] fitted in between. The prevalence of TMD's clinical positive signs of male individuals was 36.9% (73/198), and the female was 51.3% (79/154). There was a significant difference in prevalence of TMD's clinical positive signs between males and females (P = 0.0067 < 0.01). The prevalence of TMD's clinical positive signs in denture wearing group was 38.6% (91/236) and that in no denture group was 52.6% (61/116). There was a significant correlation between the prevalence of TMD's clinical positive signs and wearing denture [P = 0.0125 < 0.05, OR = 1.767 (1.130 ∼ 2.763)].

CONCLUSIONSGender and malocclusion may be the risk factors of TMD in edentulous individuals.

Aged ; Aged, 80 and over ; Dentures ; Female ; Humans ; Jaw, Edentulous ; complications ; Male ; Malocclusion ; complications ; Middle Aged ; Risk Factors ; Sex Factors ; Temporomandibular Joint Disorders ; complications

OBJECTIVETo evaluate the potential role of p38 mitogen-activated protein kinase (MAPK) in the orofacial inflammatory pain.

METHODSSD rats received subcutaneous injection of 2.5% formalin 50 µl in the left vibrissa pad to establish the inflammatory pain model. The rats were grouped into the control group, the formalin group (FOR group), the formalin + saline group (FOR + NS group) and the formalin + SB203580 group (FOR + SB group). SB203580 or saline was inserted into the rat's cisterna magna 20 minutes prior to the formalin injection, then the behavioral changes were tested. The immunofluorescence staining and Western blotting analysis were performed to examine c-fos, p38MAPK and phosphorylated p38 (p-p38) activity in Vc at 20, 60, 120, 180 minutes after formalin injection.

RESULTSp38MAPK was constitutively expressed in Vc (P > 0.05) and p38MAPK was activated following formalin injection.Compared with the control group at 20 min (0.12 ± 0.01), the level of p-p38 in FOR group (0.66 ± 0.04) and FOR + NS group (0.64 ± 0.04) increased significantly (P < 0.001). The expression of p-p38 peaked at 20 minutes, and then declined in each group. Intracisterna magna pretreatment of p38MAPK inhibitor SB203580 resulted in potent attenuation of phase II of pain behavior (P < 0.05), while the expression of c-fos was also inhibited, especially at the point of 120 min (P < 0.01).

CONCLUSIONSActivation of p38 mitogen-activated protein kinase played a major role in the development of orofacial inflammatory pain and it was verified by the experimental result that p38MAPK inhibitor SB203580 inhibited the formalin-induced orofacial pain.

Animals ; Anti-Inflammatory Agents, Non-Steroidal ; pharmacology ; Behavior, Animal ; Enzyme Inhibitors ; pharmacology ; Facial Pain ; chemically induced ; metabolism ; Formaldehyde ; Imidazoles ; pharmacology ; Male ; Phosphorylation ; Proto-Oncogene Proteins c-fos ; metabolism ; Pyridines ; pharmacology ; Rats ; Rats, Sprague-Dawley ; Trigeminal Caudal Nucleus ; metabolism ; p38 Mitogen-Activated Protein Kinases ; metabolism

OBJECTIVETo investigate the ability to form new bone and cartilage tissues of bone marrow mesenchymal stem cells (BMSC) derived from human condyle in vivo, to search the new source of seed cells in constructing tissue engineering condyle.

METHODSBone marrow was collected from the irrigation solution from resected human condyle, and was isolated by density gradient centrifugation and then purified by adherent separation and cultured in vitro. P3 or P4 BMSC populations were induced into osteoblasts and chondroblast under inductive medium in vitro and then seeded on porous coral scaffolds. The appearance and affinity of cells were investigated via scanning electron microscope. And then osteoblast or chondroblast/coral scaffolds composites were implanted into the dorsum of nude mice. The mice were sacrificed by anaesthesia overdose at six and nine weeks after surgery and the scaffolds were removed for analysis.

RESULTSScanning electron microscope showed that BMSC were adhering to the surface of coral and having an overlapped growth or to contact each other as net and stride over the pores. The in vivo scaffold specimens maintained the initial shape of the coral scaffold. The new formed bone tissues were clearly evident and islands of cartilage tissues were also found at nine weeks after implantation.

CONCLUSIONSThese BMSC derived from human condyle possess the ability of forming bone and cartilage tissues when being implanted in vivo, and can be used as a kind of seed cells in constructing tissue engineering condyle.

Animals ; Anthozoa ; Cartilage ; cytology ; Cell Proliferation ; Cells, Cultured ; Chondrocytes ; cytology ; Chondrogenesis ; Humans ; Mandibular Condyle ; cytology ; Mesenchymal Stromal Cells ; cytology ; Mice ; Mice, Nude ; Microscopy, Electron, Scanning ; Osteoblasts ; cytology ; Osteogenesis ; Random Allocation ; Tissue Engineering ; methods ; Tissue Scaffolds

OBJECTIVETo analyze the relationship between Class III malocclusion and pathological changes in temporomandibular joint (TMJ) structures using magnetic resenonce imaging (MRI).

METHODSTwenty-four Class III malocclusion adult patients and 10 normal control cases were included in the study. The characteristics of lateral pertygoid muscle (LPM) in the sample group and the control group were assessed.

RESULTSMore pathological changes of LPM were found in Class III malocclusion adult patients (36 TMJ). The changes included hypertrophy, atrophy and contracture. And there was no relation between the pathological changes of LPM and the symptom of temporomandibular disorders (TMD).

CONCLUSIONSThe frequency of pathological changes of LPM was greater in patients with Class III malocclusion than in the control group.

Adolescent ; Adult ; Atrophy ; pathology ; Case-Control Studies ; Contracture ; pathology ; Female ; Humans ; Magnetic Resonance Imaging ; methods ; Male ; Malocclusion, Angle Class III ; complications ; pathology ; Pterygoid Muscles ; pathology ; Temporomandibular Joint ; pathology ; Temporomandibular Joint Disorders ; complications ; pathology ; Young Adult

Humans ; Precision Medicine ; Temporomandibular Joint Disorders ; diagnosis ; psychology ; surgery ; therapy

Oral Medicine ; education ; trends ; Periodicals as Topic

Humans ; Immunoglobulin E ; Mouth Diseases ; Syndrome

Dental Pulp Cavity ; Dental Pulp Diseases ; therapy ; Humans ; Root Canal Preparation ; Root Canal Therapy