Nitric oxide (NO) plays an important pathophysiological role in osteoarthritis and cartilage metabolism. To determine the relationship between NO and the synthesis of type II collagen in cartilage, we measured levels of type II collagen by ELISA and procollagen (II) mRNA by RT-PCR in cultured lapine chondrocytes that were incubated with some kinds of reagents. 0.2mM sodium nitroprusside (SNP, a NO donor) can release high levels of NO, decreasing type II collagen, suppressing the expression of procollagen (II) mRNA (COL2A1).At the same time, chondrocytes showed a large increase in NO synthesis, a decrease in type II collagen and COL2A1 mRNA in response to 100u/ml IL-1. When 1mg/ml N-nitro-L-arginine methyl easter (NAME, an inhibitor of NO synthase) was mixed with IL-1, NO production was inhibited, the amounts of type II collagen recovered partially and COL2A1 mRNA recovered completely.These data indicate NO can inhibit type II collagen synthesis as IL-1 downstream molecule by suppressing procollagen (II) mRNA.

Objective To observe the articular cartilage injuries complicated with the rupture of anterior cruciate ligament(ACL)in 21 athletes and 43 non-athletes,and investigate the cause and the pattern of cartilage of knee following the ACL rupture in those patients.Methods The pathologic change,location,degree of cartilage lesion were observed. The relationship between the incidence, occurrent time,degree of cartilage injury and ACL rupture,injured degree of cartilage and the course of ACL rupture were studied.Results The incidence of cartilage injury were 75% in all patients, 66.7% in athletes and 79%in non-athletes. Incidence of cartilage injury in non-athletes was significantly higher than that in athletes (P<0.01).Incidence of serious injury of cartilage in the course more than 1 year was significantly higher than that less than 1 year (P<0.01),but there were no statistical difference between the two groups.Conclusion The incidence of articular cartilage injury following ACL rupture were significantly raised in athletes and non-athletes. The longer the ACL ruptured, the more serious the cartilage injured. Results indicated that articular cartilage injuries in the ACL rupture knee were mainly caused by the instability of knee.

20 normal males were examined by BIODEX isokinetic test and rehabilitation system,and data collected were used to observe the influence of angle and time on the isokinetic concentric constriction of knee joint. It was found that angle and time, especially angle factor obviously affected the testing results.

To understand the present situation of sport participation in community residents of Shanghai，study infecting factors to the regular sport participation rate and evaluate recognition degree of the relationship of sport and chronic disease，we carried out questionnaire survey indoors among the permanent residents,aged 35～74,in East Nanjing Road community, including survey on chronic disease and sport participation situation. The material was statistically analyzed and the related factors were proved by x2 test. The results showed that in 1457 permanent residents,470 regularly participat in sport, and were classified as sport population. The prevalence was 32.3%.The gender, age, education level, occupation and chronic disease had statistical significance (P<0.05) and they were probably infecting factors of the regular sport participation rate. Currently,the regular sport participation rate in community is relatively high and the purpose of sport participation was evident, however, the age structure of sport population was imbalance. The sport participation in residents was individualized and without proper scientific instruction.

To study the effect of physical fitness exercise prescription on the cardiac function of collegians, three different exercise prescriptions on the collegians extra-curriculum exercises to develop their strength, cardiac function and overall physical fitness separately and one control were applied. The results show that after 8 weeks training, the rest HR of all exercise groups decreased while SV(stroke volumn), SI (stroke index), CO (cardiac output)and CI (cardiac index) increased significantly. Furthermore, the results also show that VPE (ventricular pumping effectiveness) and VPEI (ventricular pumping effectiveness index) of exercise groups increased while MSP(mean systolic pressure), MDP(mean diastolic pressure) and MAP (mean arterial pressure) decreased at variant degree. From the study it is not difficult to see that doing exercise prescription can develop the collegians' cardiac-function significantly. Comparing these three exercise prescriptions show that doing endurance exercise prescription can get the best effect on developing the cardiac-function, but doing the comprehensive-developing exercise prescription can get more comprehensive effect on cardiac and vascular function. Another result from comparing the control group shows that the females are easier to get the physiological function descent than the males if they do not do exercises actively. In conclusion, it is worth to extend physical fitness exercise prescription in collegians' extra-curriculum exercise because of the simple condition, scientific method and significant effect.

Three hundred and sixty five male senior citizens were divided into experimental group(183 men)and control group(182 men).Exercise in experimental group was carried out with individual prescription for one year and the exercise in control group was in accordance to subjects' habitual interest. The results after 1 year showed that the anthropometric parameters between two groups revealed no significant difference, while the functional parameters were significantly improved in experimental group as compared with that in control group(p＜0.05 or 0.01).It was suggested that for senior citizens, exercise under prescription could significantly improve body function as compared with that without prescription.

In this paper,we reported the results of 36 cases with lumbar disc prolapse through conservative treatment in order to observe the relationship between the effect of conservative treatment and the results of CT scan.We found that the effectiveness of treatment was related to the size and location of the disc protrusion as well as the course of disease.The results suggested that these factors should be considered fully during selecting the methods of conservative treatment or operation in lumbar disc prolapse.

To appraise the preventive and improvable effects of different movements on OA joints,animal models by fixing the left posterior knee joint in extention using plaster cast for 8 weeks in Newzealand rabbits were carried out. The rabbits were randomly divided into different groups, and the X-RAY、range of motion and ligament biomechanical features of the joint were observed.The results showed that proper movement improved the range of motion, restored tensile strength of the ligament around the OA joint.It is evident that movement improves the function of the OA joint and delays the OA exacerbation.The combination of active and passive movement is the best way to achieve good result.

Partial fibulectomy is widely used in fibula transplantation and the treatment of fibular tumor and injury. After follow-up surveys and biomechanical experiments, many authors believe it is important to pay attention to the postoperative complications in leg，which now still remains in dispute. For the first time finite element analysis is used to find out the mechanical characteristics of crural bone before and after partial fibulectomy. Our results show that resection would alter significantly the stress distribution in tibia, fibula and the medial & lateral parts of talus, so influent the ankle joint stability. Causes of postoperative complications were also discussed.

The purpose of this study is to investigate the effects of phospholipids supplementation on nutritional status and antioxidant status of trained mice. The results indicate that: 1)In phospholipids supplementation groups concentrations of Hb and serum phospholipid increase significantly, serum Alb concentration tends to increase.Phospholipids supplementation can also help the recovery of Hb which is decreased after exhaustive exercise.2)Phospholipids supplementation can improve antioxidative status in some tissue .3)The activity of SOD is highest in quadriceps while the activity of GPX is highest in liver, and concentration of MDA is lowest in kidney.Different kinds of tissue show different levels of SOD, GPX and MDA, indicating their different abilities of antioxidation.4) Phospholipids supplementation have no effect on body weights of trained mice.