It is generally considered that there were no synovial membranes covering the surfaces of the meniscus. Therefore, the existence of synovial membrane is of importance in the repairing of menisci (20 menisci altogether) studied by using histological methods. The staining methods included hematoxylin and eosin(HE), Mallory and VG(van Gieson)specific technique. The main results are as follows:1. On the horizontal sections of the menisci, it shows clearly that the synovial membranes at the interior margin of the menisci is different from that of the other part.2. On the frontal section of the menisci, there are synovial membranes covering the superior and inferior surfaces of the menisci, especially evident on the inferior surface.3. On the other section of the menisci, it is observed that the synovial membrane of capsule is closely associated with that of menisci. The latter is the continuation of the former.4. On sections to which Mallory and VG staining methods were applied, the synovial that covered the menisci were observed to be the continuation of that the capsule.

Objective To develop a method for quantitative real-time detection of perforin(PFR) using polymerase chain reaction(PCR).Methods Gene expression of PFR in peripheral blood mononuclear cell(PBMC) from 25 healthy individuals was detected by PCR.An endogenous"housekeeping" gene GAPDH was used to normalize the results.Results and Conclusion The spontaneous PFR mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r=1.

0.05),but lean body weight(LBW) and total body water(TBW) increased significantly(P0.05),whereas LBW and TBW decreased significantly(P0.05),whereas LBW and TBW increased significantly(P

group C,CORT value in group I was significant higher than in the other two.In group I+S,the CORT and ACTH concentrations were lower than that in group I,but higher than that in group C and C+S.Conclusion Chronic psychological stress could induce stress-related hormone excretion and action alteration.Exercise to some degree could mitigate the psychological stress reactions.

Objective Through morphometric methods,the effects of soy hydrolyzed peptides(SHPs) supplementation on histological and ultrastructural damages of skeletal muscle after intensive training were investigated.Methods 150 male Sprague-Dawley rats were randomly divided into 15 groups according to different supplementations(placebo,ISP and SHPs),exercise or not and different sampling phase after exercise.Rats in exercise groups ran 120 minutes at speed of 20?1m /min and-16? of slope.All rats were respectively fed with 2ml beverage containing 15% SHPs,15% ISP or placebo for 1 week before exercise.Results HE staining results showed that the skeletal muscle structure changed obviously in 24～48hr after exercise and the ultrastructure of skeletal muscle changed evidently in 12～24hr after exercise.With ISP or SHPs supplementation,the histological and ultrastructural damages of skeletal muscle reduced obviously.Moreover,SHPs supplementation showed better protective effects than ISP supplementation.

0.05). Conclusion Treadmill running with low load for 1 week can enhance neurogenesis in the dentate gyrus of young SD rats.

Objective To observe the survival of myoblasts carrying hIGF-1 gene transplanted into the mice, and the expression of hIGF-1 in the transplanted mice. Methods Eighty four male C3H mice(20～30g,7～11w)were divided into four groups: group A, B, C and D (20 mice each group), and the remaining four mice were used as normal control. At the middle of the right gastrocnemius muscle, the mice in group A and B were injected with 1?10~6 myoblasts either carried with or without hIGF-1 gene. Muscle contusion at the middle of the right gastrocnemius muscle of the mice in group C and D was produced. At day 3 following injury, they were injected with 1?10~6 myoblasts either carried with hIGF-1 gene (group C) or without hIGF-1 gene (group D). At the day 2, 5, 10, 20, 30 after injection, four mice of each group were sacrificed randomly. BrdU staining in all mice were performed to evaluate cells surviving, and the expression level of hIGF-1 in group A and C was assessed by immunohistochemical staining and real-time-PCR. Results The BrdU staining in both normal and injured mice transplanted with myoblast carried with or without hIGF-1 were positive. hIGF-1 was expressed and secreted in both group A and C. Conclusion The myoblast carrying hIGF-1 gene transplanted into normal or injured mice can survive for a certain period of time, and can secrete hIGF-1.

Objective To present the special arthroscopic all-inside suture technique for repairing medial meniscus posterior horn tears and clinical follow-up results including arthroscopy and MRI outcome. Methods Eighty nine cases of posterior horn tears of medial meniscus combining with anterior cruciate ligament injuries were enrolled in this setting.Arthroscopic all-inside suturing medial meniscus through 2 posteromedial portals and 1 transcondylar portal was performed, with simultaneous ACL reconstruction using hamstring or B-PT-B autograft or allograft. Results Seventy five cases(84.3%)were followed-up for an average of 20.2 months, in which 25 cases underwent arthroscopic evaluation and 21 MRI evaluation.All 25 cases undergoing second-look arthroscopies were completely healed.Of the 21 cases receiving MRI evaluation, 18 were completely healed and 3 were partialy healed. Conclusions Arthroscopic all-inside suture technique is a good alternative for repairing medial meniscus posterior horn tear and a higher healing rate can be obtained using such a technique.

Objective To study the effects of cardiac and pulmonary response in pailents with chronic obstructive pulmonary disease(COPD) on their exercise capacity.Methods Thirty four patients with COPD(including 8 patients in stage Ⅰ,15 patients in stage Ⅱ,10 patents in stage Ⅲ and 1 patent in stage Ⅳ and 24 healthy controls performed incremental exercise testing.Oxygen uptake(VO_2) and carbon dioxide output(VCO_2)were measured breath-by-breath.Arterial blood samples were drawn both at rest and peak exercise.Results The VO_2 at peak exercise was significantly lower in COPD group than in control group(15.81±3.65ml/min/kg vs 18.96±6.10ml/min/kg,P=0.042).The respiration reserve was lower in COPD patients compared to controls.The rBorg dyspnea score at the peak exercise for COPD patents was 4.24±2.01,significantly higher than that for controls(1.754±1.50).Oxygen pulse and heart rate reserve had not statistically difference between COPD group and control group.Significant increase of PO_2 and PCO_2 were found during exercise,which were 26.374±15.40 vs 10.26±22.65 mmHg and 42.33±3.65 vs 40.29±4.46 mmHg respectively compared to at rest.Conclusion Abnormal pulmonary response to exercise might be the main cause inducing exercise intolerance in patents with COPD.

Objective To explore the exercise induced immunity changes during incremental load training from the view of the expression of helper T cells (Th) mRNA. Methods Using the technique of real-time quantified PCR to observe T-helper-special cytokine mRNA of peripheral blood mononuclear cell dynamically in healthy young male who have been trained with incremental exercise for five weeks. Results There was no obvious change in IL-2 mRNA during five weeks training. Meanwhile IL-4 mRNA increased greatly in the second, third and fourth week. IFN-? mRNA as well as IFN-? mRNA/IL-4 mRNA increased remarkably in the third, fourth and fifth week . Conclusion The expression of T-helper1/ T-helper2 cytokine mRNA were up-regulated after five weeks and the subjects were gradually adapt to the increased load which indicated the enhancement of immunity function .