Objective To study the active secondary metabolites from marine microorganism FIM02-635. Methods The producing strain was identified by taxonomical and phylogenetic studies. Two compounds FW635I1 and FW635I2 with immunosuppressive activities were extracted by organic solvents from the culture broth and purified by silica gel column chromatography and high speed counter current chromatography. The structures of the two compounds were determined by physico-chemical properties and spectral analyses,the biological activities were assayed in vitro. Results and Conclusion The producing strain was named as Micromonospora carbonacea FIM 02-635. Two compounds FW635I1 and FW635I2 were determined to be isoflavone Daidzein and Genistein, respectively, showed immunosuppressive and antitumor activities, but not antimicrobial activities.

Objective To study the single and combined-enzymic hydrolysis conditions of Oreochromis niloticus skin collagen.Methods Based on the inhibitory activities of six kinds of single enzymic hydrolysates to angiotensin converting enzyme(ACE),bromelain and alcalase(2.4L) were selected as combined-enzyme.The optimal hydrolytic conditions of the skin collagen with the two enzymes were determined by orthogonal test L_(16)(4~(5)).Results and Conclusion The results showed that the optimal hydrolytic conditions of bromelain and alcalase 2.4L were temperature 45℃,pH4.5,E/S 4000U?g~(-1),time 4h,concentration of substrate 6%;and temperature 55℃,pH7.5,E/S 6000U?g~(-1),time 2h,concentration of substrate 4%,respectively.Furthermore,hydrolytic effect would be better when using bromelain in advance,followed by alcalase 2.4L.The hydrolytic degree of the hydrolysates prepared under the provided technology was 30.43%,the ACE inhibitory rate would be as high as(68.65%) in vitro.

Objective To study antitumor and hemolytic activities of nobiliside B from the sea cucumber Holothuria nobilis Selenka and its acetylic ramification.Methods Triterpene glycoside nobiliside B was acetylated with pyridine and Ac2O in icy bath,the acetylic products were separated by multi-chromatography to afford acetoxy-nobiliside B(2).The cytotoxic activity of the glycosides against ten human tumor cell lines was evaluated with the sulforhodamione B(SRB) protein assay.Results On the basis of chemical method,spectroscopic analysis and comparison with the data of nobiliside B,the structure of compound 2 was elucidated as: 3-O-[6″-acetoxy-?-D-glucopyranosyl(1→2)-4′-O-sulfate-?-D-xylopyranosyl]-holosta-9-ene-22,25-epoxy-3?,17?-diol-sodium salt.The bioassay results showed that the hemolytic activity of compound 2 was significantly decreased,while the antitumor activity was kept.Conclusion One novel acetoxy compound with high antitumor activitiy and less side effect was obtained by structure modification of nobiliside B,and should be study as a new potential antitumor drug.

sodium carboxymethylcellulose.Their complexes also obtained the stongest adhesive strength in their saturated water solutions,but their adhesive strength was between their component colloid materials.The complexes integrate the characteristics of the three colloid materials and would provide valuable complex materials for the study of bone adhesives.

Objective To investigate the effects of conjugated linoleic acid(CLA)on survival rate of vascular endothelial cells(VEC).Methods The survival rate of VEC was assessed using MTT method after treating with CLA.Apoptosis and necrosis of the cells were determined using annexin V-propidium iodine double staining.Results CLA significantly increased the cell survival rate to 126.3% after treating for 72h at a dose of 2?mol?L-1,survival in a time-and dose-dependent manner when the concentration was less than 5?mol?L-1.CLA could partly prevent the death of VEC induced by PA and SA.Conclusion CLA can partly prevent the death of VEC induced by PA and SA.The results suggest that CLA are important for the survival of vascular endothelial cells.

Objective To study the antifungal metabolites from marine microorganism FIM03-1149.Methods The producing strain was identified by taxonomical studies.The antifungal compound FW03-1149 was extracted by organic solvents and purified by silica gel column and preparative HPLC from the culture broth of FIM 03-1149.The structure of FW03-1149 was determined by MS data analysis and physico-chemical properties.Results and Conclusion The producing strain was named as Micromonospora sp.FIM03-1149.Compound FW03-1149 was determined to be neorustmicin,showed strong antifungal activities.

Objective To determine the antibacterial activities of the alginate impression materials impregnated with carboxymethyl chitosan.Methods Carboxymethyl chitosan with different replace positions,replace degrees,relative molecular masses and deacetyl degrees were added into the dental alginate impression materials by different ratios.Their antibacterial activities were determined using a method according to the Chinese and Japanese standards for testing antibacterial efficacy of antibacterial materials.Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC6538 were used in this test.Results O-carboxymethyl chitosan showed best antibacterial activity.O-carboxymethyl chitosan added into the alginate impression materials had the best antimicrobial activity on Escherichia coli and Staphylococcus aureus in the ratios of 1% and 1.4%,respectively.Its inhibitory bacteria rate could reach 99.99% along with the increase of deacetyl degree.Conclusion The alginate impression materials impregnated with carboxymethyl chitosan have a favorable antimicrobial activity.

Objective To study on glutathione and glutathione-related enzymes of Antarctic ice microalgae.Methods Glutathione(GSH) content,glutathione produce ability(GPA) and glutathione reductase(GR) activity in 24 kinds of Antarctic ice microalgae were assessed using the spectrophotometer methods.Results GSH content of Cyanophyceae B-1 was highest.Total GSH yields of Antarctic ice microalgae Chlamydomonas sp.ICE-L and Berkeleya rutilans GJ01 were in the top two positions.GPA in most Antarctic ice microalgae was higher than that in mesophilic green algae,and only in two ice microalgae was lower than that in Phaeodactylum tricornutum.GR activities of Antarctic ice microalgae Berkeleya rutilans GJ01 and Chlamydomonas sp.ICE-L were greater than that of the control microalgae.Conclusion It is possible that Antarctic ice microalgae will become a new resource of GSH.

Objective To determine the content of octopamine,a kind of ?3-adrenergic receptor agonists in edible part of various fresh water and seawater aquatic products,and provide data for exploitation and utilization of octopamine in aquatic products.Methods After homogenate and ultrasound treatment,edible parts of fish,shellfish and cephalopodium were centrifugalized,then octopamine content in supernatant liquid was detected with HPLC.Results In eight kinds of fresh water fish,the octopamine contents of dorsal meat were generally higher than abdominal meat,which were in the range of 30~130?g?g-1 and roughly similar to seawater fish and freshwater shellfish.The octopamine contents in seawater shellfish were particularly high,almost dozens even thousands times of those in fish and freshwater shellfish.Cephalopodium also had a high content of octopamine while it was not detected in ocean algae.Conclusion Seawater mollusks are the most affluent resource of octopamine in aquatic products and ideal food of weight-watchers and diabetics.

A RP-HPLC method for the determination of TTX in the liver of Fugu vermicularis was established.The operating conditions were Hpersil BDS C18(5?m,250mm*(4.6mm) i.d),mobile phase of phosphate with Heptane-1-sulfonic acid sodium salt as an ion-pair reagent and diode array detector.The linear relationship of calibration curve was good in the range of 2.5～250?g?mL~(-1)for TTX.The detection limit was 40ng,and the relative standard deviation was 2.24%～2.68%,and the recoveries ware 79.17%～84.38%,the intra-day precision was 1.782%(n=5),and the inter-day precision was 4.554%(n=5).This method was simple,accurate and sensitive.