Fifty-two cases of acute leukemia were treated with chemotherapy, and forty-one cases in combination vrith Spirulina platensis. After the therapy, the patient's bone marrow was inhibited. The inhibition period of bone marrow was shortened and the recovery period was advanced in the combined Spirylina platensis therapy, the level of peripheral blood cells was raised. The results suggest that the combined spirulina platensis therapy in chemotherapy could accelerate the recovery of the normal hematopoiesis and increase the amounts of blood cells.

Objective To search for novel antifungal structures produced by marine fungus.Methods Using the Pyricularia oryzae (P.oryzaae) bioassay method to screen marine fungi led to the isolation of marine fungus 09-1-1-1.Active metabolites were isolated from the broth of the marine fungus 09-1-1-1.Results Two compounds were extracted from the mycelium and their structures were elucidated by various spectroscopic methods as 3a,12c-dihydro-8-hydroxy-6-methoxy-7H-furo\furo\xanthen-7-one(Ⅰ,sterigmatocystin) and 1,3,6,8-tetrahydroxy-2-(1-hydroxyhexyl)anthraquinone(Ⅱ,averantin).Conclusion The P.oryzae bioassay was a cheap,quick and convenient method for the screening of bioactive substances from marine fungi.Compound Ⅱ showed potent inhibitory activity to the mycelia of P.oryzae.The MIC of compound Ⅱagainst P.oryzae was 1.6 ?g?mL~(-1).

Objective To study the single and combined-enzymic hydrolysis conditions of Oreochromis niloticus skin collagen.Methods Based on the inhibitory activities of six kinds of single enzymic hydrolysates to angiotensin converting enzyme(ACE),bromelain and alcalase(2.4L) were selected as combined-enzyme.The optimal hydrolytic conditions of the skin collagen with the two enzymes were determined by orthogonal test L_(16)(4~(5)).Results and Conclusion The results showed that the optimal hydrolytic conditions of bromelain and alcalase 2.4L were temperature 45℃,pH4.5,E/S 4000U?g~(-1),time 4h,concentration of substrate 6%;and temperature 55℃,pH7.5,E/S 6000U?g~(-1),time 2h,concentration of substrate 4%,respectively.Furthermore,hydrolytic effect would be better when using bromelain in advance,followed by alcalase 2.4L.The hydrolytic degree of the hydrolysates prepared under the provided technology was 30.43%,the ACE inhibitory rate would be as high as(68.65%) in vitro.

Objective To detect the antiviral effect and its antiviral mechanism of the sulfated polysaccharide SP2 isolated from the brown alga Sargassum patens C. Ag. against RSV.Methods The antiviral activity,cytotoxicity and its possible antiviral mechanism were assayed by cytopathogenic effect (CPE) inhibition assay,MTT assay and plaque reduction assay. Reduction assay when it was added during virus adsorption. It did not show any antiviral activity against influenza A and B viruses and parainfluenza type 3 virus up to the concentration of treatment of cells with SP2 did not protect the cells from RSV infection. This polysaccharide addition assay showed that when SP2 was present only during virus adsorption phase at 37℃, it could significantly inhibit RSV-induced plaque formation. Conclusions SP2 might be a potent substance for treating RSV infection. The mode of action of SP2 might depend on inhibiting RSV attachment to host cells.

Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .

Objective To analyze the chemical composition of Porphyra haitanensis cultivated in Fujian Province.Methods The contents of crude protein,carbohydrate,crude fat,ash,free amino acids,inorganic elements,chlorophyll a,carotenoid,phycoerythrin and phycocyanin of dried P.haitanensis were determined by routine analysis methods.Results It showed the contents of crude protein,carbohydrate,crude fat and ash of dried P.haitanensis were 34.88%～37.21%,46.86%～49.27%,1.68%～2.09%and 8.75%～9.92%.The contents of chlorophyll a,carotenoid,phycoerythrin and phycocyanin in P.haitanensis were 60 1～646,120～130,1289～1802,1255～1743mg(100g)-1.P.haitanensis is rich in free amino acids and inorganic elements,i.e.K,Na,Mg,Fe,P and Zn,but very little heavy metals,i.e.Cu,Cd and Cr.Conclusions The P.haitanensis was a kind of health food,which was in high content of protein,low content of fat and rich of inorganic elements.

The compounds (8R23, 9R221 , 9R6222, 10R221 , H1105) extracted from the soft coral, with unsaturated lactone, showed antitumor effect on EAT, IC50 was 18. 6,85. 8,67. 8,3d. 9, 3.05umol. L-1, respectively; IC50 on S180 was 10. 7,44. 4,30. 9,9. 95,2. 4umol. L-1, respectively. 8R23, 10R221 obviously increased the level of cAMP in the EAT cell. 8R23, 10R221 , III 105 also inhibited DNA synthesis of the EAT cell.These results indicated that these compounds might have antitumor effect, but the mechanism was unknown.

We use chitosan and n -acetyl -d -glucosamine to inhibit fibroblast proliferation which was cultured in vitro. The result shows that n - acetyl - d - glucosamine: ID50=16. 35mg/L, chitosan: ID50=69. 59mg/L. We also plant the chitosan membrane to the rabbit body to test its bio-degradation. The result shows that the molecular weight of chitosan drops 17% and 6% after 25 days and 18 days, respectively. On the basis of these results, we have obtained a conclusion that we can probably use chitosan to continuously release n-acetyl -d-glucosamine which can inhibit fibroblast proliferation effectively.

The anti-mutation effect of flesh of clam Merectix meretrix Linnaeus was studied . The increase in the rate of micronuclei of mice bone -marrow polychromatic erythrocytes induced by Cy (25 mg/kg X 2d, ip) or Ur (0. 5 g/kg X 4d , ip) was inhibited by decoctio of flesh of Meretrix merelrix Linnaeus (20g/kgX7d, ig).

This paper reports the effects of the polysaccharide from Sargassum fusiforme (SFPS) on contents of lipoid peroxide (LPO) and enzyme activities of glutathione reductase (GR), glutathione peroxidase (GSH - PX), catalase (CAT), superoxide dismutase (SOD) in whole blood, liver and spleen in leukemia L615 mice. The results show that SFPS remarkably reduces the contents of LPO and increases in the enzyme activities of CAT , SOD in whole blood, liver and spleen in leukemia L615 mice. The authors point out that SFPS may remove the free radicals in the body of leukemia L616 mice and have an effect against lipid peroxidation. This may be one of the mechanisms of SFPS against leukemia.