Objective To detect the antiviral effect and its antiviral mechanism of the sulfated polysaccharide SP2 isolated from the brown alga Sargassum patens C. Ag. against RSV.Methods The antiviral activity,cytotoxicity and its possible antiviral mechanism were assayed by cytopathogenic effect (CPE) inhibition assay,MTT assay and plaque reduction assay. Reduction assay when it was added during virus adsorption. It did not show any antiviral activity against influenza A and B viruses and parainfluenza type 3 virus up to the concentration of treatment of cells with SP2 did not protect the cells from RSV infection. This polysaccharide addition assay showed that when SP2 was present only during virus adsorption phase at 37℃, it could significantly inhibit RSV-induced plaque formation. Conclusions SP2 might be a potent substance for treating RSV infection. The mode of action of SP2 might depend on inhibiting RSV attachment to host cells.

Objective To search for triterpene glycoside with new structure. Methods We studied the sea cucumber Colochirous anceps belonging to the family cucumariidea with many chromatography methods and found a new sulfated triterpene glycoside, which structure was established by a combination of spectroscopic analysis (IR, UV, ESI-MS and 2D-NMR), chemical transformations and other chemical evidence. Results and Conclusion The new sulfated triterpene glycoside was isolated and named Colochiroside A (1) .

Objective To analyze the chemical composition of Porphyra haitanensis cultivated in Fujian Province.Methods The contents of crude protein,carbohydrate,crude fat,ash,free amino acids,inorganic elements,chlorophyll a,carotenoid,phycoerythrin and phycocyanin of dried P.haitanensis were determined by routine analysis methods.Results It showed the contents of crude protein,carbohydrate,crude fat and ash of dried P.haitanensis were 34.88%～37.21%,46.86%～49.27%,1.68%～2.09%and 8.75%～9.92%.The contents of chlorophyll a,carotenoid,phycoerythrin and phycocyanin in P.haitanensis were 60 1～646,120～130,1289～1802,1255～1743mg(100g)-1.P.haitanensis is rich in free amino acids and inorganic elements,i.e.K,Na,Mg,Fe,P and Zn,but very little heavy metals,i.e.Cu,Cd and Cr.Conclusions The P.haitanensis was a kind of health food,which was in high content of protein,low content of fat and rich of inorganic elements.

The developments in the study of marine malabaricane triterpenes were reviewed in this paper. The relationships between chemical structures and biological activities of marine malabaricane triterpenes were also described.

Objective To study the protective effect of PCF against murine thymocytes apoptosis induced by UVB irradiation.Methods The irradiation damage model of thymocytes caused by UVB was established.Cellular ultrastructuralalterations were observed with TEM.The expressions of P53,Bax and Bcl-2 proteins were examined by immunocytochemical technique. The p38 mRNA gene expression was also determined with the technique of in situ hybridization.Results Apoptotic thymocytes were observed after UVB irradiation.The ultrastructural damages of the cells induced by UVB irradiation were reduced by the treatment of PCF.PCF also raised the expression of Bcl-2 gene and decreased the expression of P53,Bax.The mRNA expressions of p38 were decreased in PCF groups.Conclusion PCF showed significant protective effects on UVB-irradiated murine thymocytes in vitro.

Objective To investigate the influences of Rhodospirillaceae on immune function of mice.Methods Four tests were performed in mice after 4 w medication with different doses of Rhodospirillaceae ig,including:1.The transformation of spleen lymphocyte(MTT method);2.The phagocytosis of macrophagocyte(semi-vivo method);3.The value of HC50; 4.The activity of NK cell(LDH method).Results1.The transformation of spleen lymphocyte;2.The phagocytosis of macrophagocyte,the Value of HC50 and the activity of NK cell in test groups were all raised,the differences were significant and very significant when compared with the control group.Couclusions Rhodospirillaceae could enhance the cellular and humoral immunity of mice.

To investigate the effects of C-phycocyanin(C-PC) from Spirulina platensis on erythropoiesis in mice.The colony forming uniterythroid(CFU-E) and burst forming unit-erythroid(BFU-E) were determined using micro-methycellulose culture method in vitro.The erythropoietin(EPO)-like activity of C-PC was examined using the technique of CFU-E culture of fetal liver cells in mice in vitro.Anemic mice models were established by ~(60)Co ?-ray irradiation(5Gy) and intra-peritoneal injected with benzohyarazine hydrochloride.After normal mice were intra-peritoneal injected with C-PC (50 mg?kg~(-1)) for 5d,C-PC provided the only increase in the numbers of CFU-E and BFU-E-derived colonies.C-PC exhibited higher EPO-like activity.12.5 ng of C-PC could match with EPO 1.06U.The specific activity of C-PC was 84,800 U?mg~(-1) C-PC.When anemic mice were intra-peritoneal injected with C-PC for 5d,on d10 the leukocyte,erythrocyte and hemoglobin were significant increased.

Objective To investigate the effects of polysaccharide from Porphyra on spleen cell function in immunosuppressive mice. Methods The polysaccharide at dosages 0.025, 0.050 and 0.150g?kg~ -1 was injected intraperitoneally for 7 days in the immunosuppressive mice induced by cyclophosphamide. On day 8, the splenic lymphocytic proliferation and activity of TNF were determined. Results The splenic lymphocytic proliferation and cytotoxicity of tumor necrosis factor (TNF) of the group treated with 0.150g?kg~ -1 of porphyra polysaccharide were higher than those of model group. Conclusion Polysaccharide from Porphyra could promote splenic lymphocytic proliferation and raise the level of TNF.

Objective To study the antitumor mechanism of DHA compound. Methods RT-PCR was used to investigate the effects of DHA compound on H-Ras and pl6 mRNA transcription in gastric adenocarcinoma. Results Compared with control, the content of H-Ras mRNA was decreased and the content of p16 mRNA was increased in gastric adenocarcinoma significantly after treated with DHA compound. Conclusion The transcription of H-Ras gene was decreased and the transcription of pl6 gene was increased in gastric adenocarcinoma after treated with DHA compound and thus the cancer cell growth was inhibited.

Objective The polysaccharides sulfate were extracted from three kinds of algae in-cluding porphyra tenera , Laminaria ja ponica and Sargassum fusi forme ( Harv. )Setch . in order to screen antioxidant with exploiting potential. Methods Using the assay system of DP-PH,the antioxidative activities of various extracts were studied. Results Three kinds of algae polysaccharides sulfate had different antioxidative activities. Moreover, polysaccharide extracted with different solvents had different intensities of antioxidative activity. Conclusion Polysacchride sulfate extracted with water has stronger antioxidative activity than that with others.keywolds antioxidation; porphyra tenera kjellm. ; Laminaria japonica; Sargassum fusi -forme ( Harv. )Setch.Studies on the extraction of polysaccharides sulfate from three algae and their scavenging activity on free radicalsHAN Hua,ZHOU Hai Yan,LIU Cheng-Chu,WANG Chun-bo(1.Bromatology Institute of Shanhai Aquatic University , Shanghai 200090, China; 2. Medical College , Qingdao University ,Qingdao 266021, China)Abstract:Objective The polysaccharides sulfate were extracted from three kinds of algae in-cluding porphyra tenera , Laminaria ja ponica and Sargassum fusi forme ( Harv. )Setch . in order to screen antioxidant with exploiting potential. Methods Using the assay system of DP-PH,the antioxidative activities of various extracts were studied. Results Three kinds of algae polysaccharides sulfate had different antioxidative activities. Moreover, polysaccharide extracted with different solvents had different intensities of antioxidative activity. Conclusion Polysacchride sulfate extracted with water has stronger antioxidative activity than that with others.