1.Expression and significance of erb-B receptor family in hepatocellular carcinoma.
Ji CAO ; Yin-Nong ZHAO ; Fei-Xiang WU ; Chao OU ; Ke-Chen BAN ; Wei WEI ; Yuan LI ; Jian-Jia SU ; An-Min LIANG
Chinese Journal of Hepatology 2005;13(2):146-147
Adult
;
Aged
;
Carcinoma, Hepatocellular
;
metabolism
;
Female
;
Humans
;
Liver Neoplasms
;
metabolism
;
Male
;
Middle Aged
;
RNA, Messenger
;
biosynthesis
;
genetics
;
Receptor, ErbB-2
;
biosynthesis
;
genetics
;
Receptor, ErbB-3
;
biosynthesis
;
genetics
3.The application of Bletilla striata in the interventional therapy of hepatocellular carcinoma: a comparative study using ACI rats.
Jun QIAN ; Zhuan-Sheng ZHENG ; Han-Ping WU ; Wei-Yong LI ; Daryusch VOSSOUGHI ; Oppermann ELSIE ; Vogl THOMAS ; Gan-Sheng FENG
Chinese Journal of Hepatology 2005;13(2):143-144
4.The level of serum thrombopoietin in patients with chronic hepatic diseases suffering from thrombocytopenia.
Yu-Tao XIE ; Xiao-Hua JIANG ; De-Ming TAN
Chinese Journal of Hepatology 2005;13(2):142-143
Adolescent
;
Adult
;
Aged
;
Female
;
Hepatitis, Chronic
;
blood
;
complications
;
Humans
;
Male
;
Middle Aged
;
Thrombocytopenia
;
blood
;
etiology
;
Thrombopoietin
;
blood
6.Abstinence and fatty liver.
Chinese Journal of Hepatology 2005;13(2):140-140
7.Drug therapy for nonalcoholic fatty liver disease.
Chinese Journal of Hepatology 2005;13(2):139-139
9.Current therapeutic strategy of fatty liver diseases.
Chinese Journal of Hepatology 2005;13(2):136-137
Fatty Liver
;
therapy
;
Fatty Liver, Alcoholic
;
therapy
;
Female
;
Humans
;
Male
10.Molecular mechanisms of the protection of SNMC in HepG2 cell apoptosis.
Yan WANG ; Ying-Ji MA ; Bao-Shan YANG ; Man-Ru BI ; Li-Yan CHEN
Chinese Journal of Hepatology 2005;13(2):132-135
OBJECTIVEApoptosis of the cells of liver cancer cell line HepG2 could be induced by TNF alpha and actinomycin D (Act D). In the current study, the molecular mechanism of the apoptosis protection of stronger neo-minophagen C (SNMC) to HepG2 cells was investigated.
METHODSSNMC was added to the HepG2 cell culture medium when the cell concentration reached 0, 2, 20, 100, 200, 800 microg/ml 30 min before their apoptosis were inducted with TNF alpha and Act D. A flow cytometry assay was performed to detect the cell apoptosis rate; electromicroscopy was employed to visualize the subcellular structure after apoptosis. DNA ladder formation was checked with genomic DNA agarose electrophoresis. The expression pattern of apoptosis related protein Caspase-3, Bcl-2 and Bax was detected by Western blot.
RESULTSAfter pretreatment with various concentrations of SNMC and 12 hours after treatment with TNF alpha and Act D, the HepG2 cell apoptosis rate and DNA ladder formation decreased dramatically when the SNMC concentration was higher in the media; the intracellular inactive form of Caspase-3 increased while the 17*10(3) active Caspase-3 decreased gradually. In addition, the expression of Bcl-2 increased and the expression of Bax decreased. Under the electromicroscope, the typical nucleolus condensation of HepG2 induced by TNF alpha and Act D was not seen among the 100 microg/ml SNMC treated cells.
CONCLUSIONSNMC inhibits TNF alpha and Act D induced HepG2 cell apoptosis. This protective action may be regulated by intracellular apoptosis related factors.
Apoptosis ; drug effects ; Carcinoma, Hepatocellular ; pathology ; Cell Line, Tumor ; Cysteine ; pharmacology ; Drug Combinations ; Glycine ; pharmacology ; Glycyrrhiza ; Humans ; Liver Neoplasms ; pathology ; Oleanolic Acid ; analogs & derivatives ; pharmacology