1.An Improved Model of Mouse Partial Hepatectomy for Study on Liver Regeneration
Guoming SHI ; Jiabin CAI ; Jingxian YU ; Aiwu KE ; Zhaoru DONG ; Chi ZHANG ; Pengfei ZHANG ; Shuangjian QIU ; Huichuan SUN ; Jian ZHOU ; Jia FAN
Chinese Journal of Clinical Medicine 2014;(3):237-238
Objective:To establish an improved model of mouse partial hepatectomy for study on liver regeneration .Methods :Healthy 8~10 week old male C57BL/6 mice were anesthetized by intraperitoneal injection .Then ,common hepatic pedicle of left lateral lobe of liver and middle lobe of liver was wrapped around with 6-0 proline suture .6-0 proline was fixed on the sur-face of right middle lobe of liver ,which was 0 .2 cm away from the hepatic pedicle .Hepatic pedicle was ligated and left lateral lobe and middle lobe of liver were resected 0 .2 cm away from ligature .The postoperative survival rate and the situation of liver weight vecovery at 3rd day ,7th day ,and 14th day after the operation were observed .Results:An improved mouse model of partial hepatectomy ,of which left lateral lobe of liver and middle lobe of liver were resected ,was established .The weight of removed liver accounted for 66 .7% of total liver and the survival rate of mice was 100% .After liver resection ,the compensa-tory regeneration of remaining liver achieved 58 .2% of the total liver weight at the 3rd day ,78 .0% at the 7th day ,and 93 .2%at the 14th day after the operation .Conclusions :An improved mouse model of partial hepatectomy was successfully established for study on liver regeneration ,which has advantages such as simplicity ,operability and high success rate .
2.Effect of Interferon αon the Expression and Activity of Transcription Factor Sp 1 in Hepatocarcinoma Cells
Qi PAN ; Wanyong CHEN ; Kai ZHU ; Jubo ZHANG ; Yiming ZHAO ; Xiaodong ZHU ; Huichuan SUN ; Lu NG WA ; Ning REN
Chinese Journal of Clinical Medicine 2014;(3):239-241
Objective:To evaluate the effect of interferon-αon the expression and activity of transcription factor specificity pro-tein 1(Sp1) in hepatocarcinoma cells .Methods :The effect of interferon-αon the expression and phosphorylation level of Sp1 in hepatocarcinoma cell line M HCC97H were detected by Western blotting .The effect of interferon-α on the activity of Sp1 was analyzed by electrophoretic mobility shift assay (EMSA) .Results :Interferon-α significantly reduced the expression and phos-phorylation level of Sp1 ,and the reducement on phosphorylation was more distinct .Interferon-α also inhibited the activity of Sp1 significantly .Conclusions :Interferon-αinhibits the activity of Sp1 by down regulating the expression and phosphorylation level of Sp1 .Sp1 may be an optional predictor of clinical efficacy of interferon-αon hepatocarcinoma .
3.Expression and Clinical Significance of UHRF1 in Hepatocellular Carcinoma
Guoming SHI ; Jiabin CAI ; Jingxian YU ; Aiwu KE ; Zhaoru DONG ; Chi ZHANG ; Pengfei ZHANG ; Shuangjian QIU ; Huichuan SUN ; Jian ZHOU ; Jia FAN
Chinese Journal of Clinical Medicine 2014;(3):242-244
Objective:To study the expression of UHRF1 and its relationships with the clinicopathological characteristics in hepatocellular carcinoma(HCC) .Methods :The expression of UHRF1 was analyzed in 556 cases of tumor tissues and peritumor tissues of HCC by immunohistochemistry .The relationships of UHRF1 with the clinicopathological characteristics of HCC were assayed .Results:Immunohistochemistry showed that positive staining of UHRF1 located in the nucleus of HCC cells .The ex-pression levels of UHRF1 protein were higher in tumor tissues than in peritumor tissues .HCC patients with positive expression of UHRF1 protein accounted for 59% of the whole cohort .The expression of UHRF1 protein was significantly correlated with the size of tumor ( P<0 .01) ,differentiation (P<0 .05) and microvessel invasion(P<0 .01) .Conclusions :The overexpression of UHRF1 is correlated with the malignant phenotypes of HCC and probably implicated in the invasion and metastasis of HCC .
4.Clinical Value of Qualitative Determination of C-Reactive Protein in the Differential Diagnosis of Benign and Malignant Pleural Effusions or Ascites
Qingping WANG ; Yuan SHI ; Qin HU ; Xiaojing LI ; Yingyong HOU ; Lude SUN ; Hongxian XIE ; Jieakesu SU ; Yunshan TAN
Chinese Journal of Clinical Medicine 2014;(3):245-246
Objective:To explore the clinical value of qualitative determination of C-reactive protein (CRP) in the differential diagnosis of benign and malignant pleural effusions or ascites .Methods :CRP in 3820 cases of pleural effusions and ascites were qualitatively examined by latex agglutination method .Specimen and latex reagent were added to the wells of the reaction plate successively .The result was observed after 2 minutes’ thorough mixing .The result was judged as positive if agglutination par-ticles were visible .Results:The positive rate of CRP in 672 cases of malignant pleural effusions and ascites was 6 .1% .The positive rate of CRP in pleural effusions and ascites was 75 .0% in 32 cases of hematopoietic and lymphoid tissue tumors ,and 2 .7% in 640 cases of epithelial malignant tumors .The positive rate of CRP in pleural effusions and ascites in 3148 cases of be-nign diseases was 21 .8% .The positive rate of CRP in benign pleural effusions and ascites was higher than that in malignant pleural effusions and ascites(P<0 .05) .The positive rate of CRP in pleural effusions and ascites of patients with hematopoietic and lymphoid tumors was higher than that of patients with epithelial malignant tumors .Conclusions :Qualitative determination of CRP in pleural effusions and ascites can be used to differentiate benign and malignant pleural effusions and ascites ,and to differentiate pleural effusions and ascites of hematopoietic and lymphoid tissue tumors and epithelial malignant tumors .
5.Inducing Effect of TWEAK on Generating Chemokines in Rat Articular Chondrocytes Cultured in Vitro
Guoping CAI ; Min XIONG ; Xinchao ZHANG ; Dechang LIU ; Keke GUI
Chinese Journal of Clinical Medicine 2014;(3):247-250
Objective:To explore the effect of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) on generating chemokines in rat articular chondrocytes cultured in vitro ,and to understand its role in the pathogenesis of OA .Methods :Rat articular chondrocytes were cultured in vitro .The cells of experimental group were induced by 250 ng/mL TWEAK ,while the cells of the control group were not .Levels of chemokines interleukin-8(IL-8) ,monocyte chemotactic protein 1(MCP-1) ,regu-lated upon activation normal T cell expressed and secreted factor (RANTES) ,macrophage inflammatory protein 2(MIP-2) in the nutrient solution of the two groups were detected at 24 ,48 and 72 h .Results:After the induction of TWEAK ,the levels of RANTES in the supernatant of rat articular chondrocytes at 24 ,48 and 72 h were all significantly higher than those in the con-trol groups ,respectively (all P<0 .05) ,and the contents of RANTES were positively correlated with the treatment time of TWEAK (P<0 .05) .The IL-8 and MCP-1 levels in the supernatant of rat articular chondrocytes at 48 and 72 h were signifi-cantly higher than those in the control groups (both P< 0 .05) ,but they had no correlations with the treatment time of TWEAK (P>0 .05) .The MIP-2 levels of the rat articular chondrocytes supernatant at 48 and 72 h were significantly lower than those in the control groups (P<0 .05) ,and the contents of MIP2 were positively correlated with the treatment time of TWEAK (P<0 .05) .Conclusions :TWEAK can induce rat chondrocytes in vitro to generate chemokines (IL-8 ,MCP-1 ,RAN-T ES ) ,so that it is involved in the occurance of OA .
6.The Detection of Serum CEA,CA19-9,CA72-4 and CYFRA21-1 Levels in Patients with Laryngeal Carcinoma and Its Clinical Significance
Peng WANG ; Fenghua QIN ; Li GAO ; Na SHEN ; Ying QI ; Xinsheng HUANG
Chinese Journal of Clinical Medicine 2014;(3):251-253
Objective:To explore the clinical value of serum tumor markers ,which are carcino-embryonic antigen (CEA) ,car-bohydrate antigen 19-9 (CA19-9) ,carbohydrate antigen 72-4 (CA72-4) and cytokeratin 19 fragments (CYFRA21-1) ,in early diagnosis and treatment of laryngeal carcinoma .Methods :From January 2012 to June 2013 ,60 patients with laryngeal carcino-ma (case group) were selected ,and 60 corresponding participants in physical examination were also enrolled as healthy controls in this study .The serum contents of CEA ,CA19-9 ,CA72-4 and CYFRA21-1 were measured before and after operation in the two groups .The differences of sensitivity and specificity in detection of laryngeal carcinoma were compared between individual and combined detection of targeted indicators .Results:The serum levels of CEA ,CA19-9 ,CA72-4 and CYFRA21-1 were significantly higher in the study group than those in the control group (P<0 .01) .The serum levels of CEA ,CA19-9 ,CA72-4 and CYFRA21-1 of the study group were significantly higher in pre-operation than those in post-operation ,and the differ-ences were statistically significant (P<0 .01) ,among which CA19-9 showed the highest sensitivity (60 .00% ) ,while CA19-9 and CA72-4 showed the highest specificity (both were 100% ) .ROC curve analysis showed that the area under curve (AUC) of CA19-9 was 0 .987 ,which is significantly higher than those of CEA ,CA72-4 and CYFRA21-1 .The sensitivity of combined de-tection was significantly higher than individual test ,and the differences were statistically significant (P<0 .05) .Conclusions :Detection of serum CEA ,CA19-9 ,CA72-4 and CYFRA21-1 levels can be applied to early diagnosis of laryngeal carcinoma . Combined detection can significantly improve the sensitivity in diagnosis meanwhile maintain the superior specificity .
7.Effects of Hyperthermia on the Sensitivity of Gastric Cancer Cell Lines to Chemotherapy
Fang LI ; Fanzhi KONG ; Xuzhong LI ; Lianming ZHOU ; Guangjun ZHANG ; Zhongming HUANG ; HEYifeng ; Xueli ZHANG
Chinese Journal of Clinical Medicine 2014;(3):254-258
Objective:To observe the effects of hyperthermia on the sensitivity of gastric cancer cell lines to paclitaxel (TAX) . Methods :To establish TAX-resistant gastric cancer cell line through a method of increasing concertration gradient and named it MKN-45/TAX .Immunocytohistochemistry staining method was used to detect the expression of multi-drug resistance gene (MDR1) in MKN-45/TAX and MKN-45 .CCK-8 test was used to detect the proliferation inhibition rates in two kinds of cell lines under different temperatures and different TAX concentration .Real-time polymerase chain reaction (RT-PCR) and West-ern blotting were used to detect the expression of MDR1 mRNA and P-glycoprotein (P-gp) before and after target siRNA treat-ment .Results:Establishment of MKN-45/TAX cell line was successful .The expression level of MDR1 was high in MKN-45/TAX whereas it was low in MKN-45 .As TAX concentration increased ,the proliferation inhibition rates of both cell lines in-creased .At 42 ℃ the proliferation inhibition rate of MKN-45/TAX to TAX chemotherapy decreased ,while that of MKN-45 was the opposite .Both the expression of MDR1 mRNA and P-gp decreased after the siRNA transfection .Conclusions :Hyper-thermia combined with chemotherapy may enhance the resistance of TAX-resistant gastric cancer cell line to TAX ,while it may enhance the sensitivity of TAX-sensitive gastric cancer cell line to TAX .The mechanism may be related to the expression of MDR1 .
8.The Expression of MicroRNAs in HT-29 Cells with Lipopolysaccharide-Induced Inflammation
Haibo WU ; Zhigang CAI ; Shaoming NG ZHA ; Heng ZHANG ; Lei WANG ; Xiaoping XU
Chinese Journal of Clinical Medicine 2014;(3):259-261
Objective:To study the expression of microRNAs (miRNAs) in colon adenocarcinoma cell line HT-29 with lipopo-lysaccharide-induced(LPS-induced) inflammation .Methods :HT-29 cells were treated with 400 ng/mL LPS for 48 h to establish inflammatory HT-29 cell model (LPS group) ,and HT-29 cells without LPS treatment were viewed as the control group .Total RNA from the two groups was isolated with Trizol reagent ,and IL-6 and CRP were examined .The expression of miRNAs was determined by Real-time PCR .Results:Compared with the control group ,the mRNA levels of IL-6 and CRP were up-regulated in LPS group .The expression levels of miR-22 ,miR-26 ,miR-214 in LPS group were lower than those in the control group , while miR-23 level was higher than that in the control group .The levels of miR-24 ,miR-30 and miR-181 were not statistically different between the two groups .Conclusions :MiRNAs may play important roles in LPS-induced inflammation of adenocarci-noma cells .
9.Detection of Serum Levels of Resistin and Leptin in Patients with Colon Cancer and Its Clinical Significance
Chentao LV ; Yong JIANG ; Lu HAN ; Dongxing HAN
Chinese Journal of Clinical Medicine 2014;(3):262-264
Objective:To investigate the correlation between serum levels of resistin and leptin in patients with colon cancer and clinicopathological characteristics of colon cancer .Methods :A total of 29 patients who underwent radical surgical resection for colon cancer from Mar 2013 to Dec 2013 were selected as the study group ,while 27 healthy volunteers were selected as the con-trol group .The serum levels of resistin and leptin were measured by enzyme-linked immunosorbent assay(ELISA) .The correla-tion between patients′ serum levels of resistin and leptin and their clinicopathological characteristics were analyzed .Results:The serum levels of resistin and leptin in the study group were significantly different from those in the control group (P<0 . 05) .The serum levels of resistin and leptin had no significant difference among patients with colon cancer at different stages or different differentiation degrees(P>0 .05) .Conclusions :Resistin and leptin may be involved in the occurrence and development of colon cancer .
10.Correlation between Lung Cancer and the Polymorphism of Xeroderma Pigmentosum Gene D and X-Ray Repair Cross Complementing Gene 1
Yong DU ; Zhijun JIE ; Yanchao HE ; Ling QIAN
Chinese Journal of Clinical Medicine 2014;(3):265-268
Objective:To investigate the correlation between lung cancer and the polymorphism of xeroderma pigmentosum gene D(XPD) and X-ray repair cross complementing gene 1 (XRCC1) .Methods :The polymorphisms at loci XPD-751 and XRCC1-399 in 150 patients with lung cancer (lung cancer group) and 150 healthy volunteers (control group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) .Results:There were significant differences in XPD-751 genotype frequency and mutant allele frequency between lung cancer group and control group (P= 0 .046 , P=0 .003) ,while XRCC1-399 loci had no significant difference(P=0 .395 ,P=0 .105) between the two groups .There were signif-icant differences in XPD-751 mutant allele frequency between patients with non-small cell lung cancer and patients with small cell lung cancer(P=0 .012) ,as well as among squamous cell carcinoma ,adenocarcinoma and other types of non-small cell lung cancer(P=0 .023) .When smoking and XPD-751 polymorphisms coexisted as risk factors ,the relative risk to lung cancer was 2 .812(95% CI:0 .956~6 .415) ,P=0 .336 .Conclusions :XPD-751 gene polymorphism was correlated with the occurrence of lung cancer ,especially with squamous cell lung carcinoma ,while XRCC1-399 gene polymorphism was not correlated with lung cancer .There is no interaction between smoking and the polymorphism of XPD-751 in lung cancer .