Objective To understand the gene characteristics of envelope region of human T lymphotropic virus type Ⅰ(HTLV Ⅰ)from Shantou region of China. Methods The gene of proviral envelope region of HTLV Ⅰ Shantou strain (named HTLV Ⅰ WHP)was amplified by PCR,using 4 designed primers,and then was sequenced,meanwhile it was compared with that of various HTLV Ⅰ representative strains from all around the world,and subsequently it was analyzed to construct its phylogenetic tree.Results The nucleotide sequence of complete envelope region of HTLV Ⅰ WHP provirus we have obtained was 1466 bp.There no deletion and insertion in the deducted amino acid sequence.The results of phylogenetic tree analysis showed that the HTLV Ⅰ WHP strain was most closed to some of the strains from Japan and Caribbean,and like the latter,belonged to the subtypea of cosmopolitan group.Conclusion The HTLV Ⅰ strains either from the coastal region of Southeast China or from Japan,Caribbean and so on have the common origin of evolution.

Objective To induce CD4+CD25+ regulatory T lymphocytes from mononuclear cells of rhG-CSF mobilized peripheral blood,and to analyze the phenotype and function of them.Methods Blood samples of ten donors of peripheral blood stem cell were collected,CD4+CD25–T lymphocytes from rhG-CSF mobilized peripheral blood(G-PB group) and unmobilezed peripheral blood(PB group) were isolated with a CD4+CD25+T lymphocytes selection kit,and then were induced by TGF-?1.FCM,RT-PCR,cell proliferration and suppression assay were used to test the expression of CD25+,Foxp3 and suppressive function,and the difference of the rate and the suppressive activity of CD4+CD25+T cells were compared.Results 1) After treated with anti-CD3mAb and TGF-?1,there was a difference between CD4+CD25–T lymphocytes isolated from G-PB group and PB group,the expression of CD25+ were(77.9?2.3)% and(65.7?4.2)%,respectively(P

A comparison between 70-blood-donor and 70-health-control blood lipoids,blood composition,and hemorrheological indexes by paired analysis method basedon occupation,sex and age was made.Paired T-test showed that though severalindexes of the blood donors reduced remarkably,they were still within the normalphysiological limits,There were no abnormal findings through physical examinationand ECG,indication that according to the general physique and present diet condi-tions of Chinese population.It is safe to donate 300ml blood once at intervals of three months.

A safe and effective method for separating albumin from autoplasma by heatwas introduced.Immunological analysis,showed that such an albumin solutionhad the same identity as human serum albumin.Determination by acetate cellu-lose electrophoresis showed the purity of the albumin was over 90%.Determination by polyacrylamide gel electrophoresis showed that one of thealbumin monomers was over 97%.Detection by double radial immunodiffusionindicated that there were no any new antigens in it.The recovery of the albuminwas more than 80%.

A specific monoclonal antibody(McAb),which could identfy different conformations of protein C(PC) in the presence or absence of Ca24+ .was selected and coupled with Sepharose-4B gel to form an affinity chromatographic column. When fresh plasma passed through this column.its PC would he adsorbed exclusively,and the obtained effuent was PC-deficient plasma (PCDP). The residual PC in the PCDP gained was

A standard microlymphocytotoxicity assay was used to test the sera for HLAantibodies in the patients receiving multitransfusions and to identify their speci-ficity,and at the same time the platelet-associated antibodies were detected.As a result,the dectable rate of HLA antibodies was 39.14% (119/304),of which24.37% (29/119) had specificity;the positive rat platelet-associated antibodieswas 39.83% (47/118).The frequency of positive incidence of HLA antibodiesincreased with the number of transfusions and donors,having a significant differ-ence.The nonhemolytic reaction to transfusion was associated with HLA antibodi-es.The frequency of positive incidence of HLA antibodies was the highest whenthe patients were transfused with concentrated granulocytes.There were takensome measures to prevent and reduce the transfusion reaction.

200 units of eligible blood from donors with negative HBsAg reaction had been tested for hepatitis B virus markers (HBVM), using ELISA and dot-blot hybridization. The results of testing showed that 120 of the 200 units of blood had negative HBVM reaction, and the rest 80 units of blood had positive HBVM reaction. Among these 80 units of blood, 62 units had negative hepatitis B virus replication markers (HBVRM) and the rest 18 units had positive HBVRM. The rate, of post-transfusion HBV infection in the patients received HBVM (+) blood was 25.4%, significantly higher than the rate of post-transfusion HBV infection (3.75%) in the patients received HBVM (-) blood (P

A Human blood type antigen P1 with very high activity was separated from sheep hydatid cyst fluid. Determination of the antigen by ultraviolet spectrum, polyacrylamide electrophoresis, gel filtration, and determination of its carbohydrate content and aminoacid residue had indicated that the substance was a glycoprotein, its total carbohydrate content was 80％ about one-fifth of which was hexosamine, and other carbohydrate components were glucqse, galactose and glucosamine. Its protein consisted of 14 aminoacids, and its molecular weight was about 30000 Daltons. By immunizing the animals with this substance, the anti-P1 serum of high titer could be obtained. The successful extraction of this substance P1 with high activity would provide the abundant resource for reseach on human blood type antigen and its immunochemistry, and create the good conditions for studies in preparation and application of anti-P1 serum or monoclonal antibody toward P1.

A success in reconstruction of the hematopoietic function of marrow in a case of malignant lymphoma attained by autologous peripheral blood stem cell transplantation after the ultra-high dose chemoradiotherapy was reported. The platelet count of patient was increased to over 20 ? 109/L at the eighth day after PBSC transplantation and was recovered to over 50?109/L at the tenth day after PBSC transplantation. The white blood cell count was increased to over 0. 5?109/ L at the ninth day after PBSC transplantation. The pelvic CT-graphy showed reduction in size of the tumor. And then the patient was outhospitalized due to remission,having been surviving for more than 6 months with routine follow-up. It was suggested that the autologous peripheral blood stem cell transplantation could be a safe and effective suplement therapy for radical cure of malignant tumor.

A method for partially purifying the native human gamma interferon (HuIFN-?) was presented. The rude HuIFN-?was concentrated by PEG 4 000, precipitated by (NH_4)_2SO_4 and purified by CM-Sephades C-50(more than 100 times). The titre of HuIFN-?was 1.3X10~5u/ml and its specific activity was 1. 0 X 10~5u/mg protien. Quality control tests were performed and 10 volunteers received intramuscularly the purified HuIFN-?without significant changes in body temperature, pulse rate and blood pressure. It is considered to be suitable for clinical trial.