The effect of an specific immunoglo- bulin to meningococcus group A was tes- ted in rabbits infected with meningococcus group A and yielded satifactory results All the 10 rabbits in the treated group appeared normal, while 5 of the 12 rabbits in the control group developed toxic symptom and DIC, and 2 died finnally. The immunoglobulin was then appli- ed clinically to 4 children with meningo- coccal meningitis. Three of them recove- red. Unfavorable outcome was found in the fourth patient, in whom meningococ- cus group B was isolated from his blood and CSF. Since meningococcus group A continues to be the predominant pathogen in our country, the above-mentioned study provides strong support for the adminis- tration of specific immunoglobulin to chil- dren with meningococcal meningitis.

ABO blood group of 236 patients with aplastic anemia (AA) was compared with that of 2277 healthy individuals. Subjects with A antigen were susceptible to AA (R=1.3090, x~2=3.8489, P

A total of 272 lots of immunoglobulin products was investigated, and the anti- body potency of measles was determined by hemogglutination inhibition test. The total antibody potencies of diphtheria, pertusis, rubella, epidemic hemorrhagic lever and titer of anti-HBs and anti-HAY were tested in some lots of immunoglobulin produced in recent years. The results provided a scien- tific basis for clinical use.

Investigation on anti-HCV in different cohorts of 21844 blood donors from 18 districts and counties of Beijing, and Guan, Yongqing, Xincheng, Xushui counties of Hebei province had been performed. It was showed that the anti-HCV-positive rates of voluntary and nonpaid donors were 1.7% (13/738) and 2.9% (116/3943) respectively, and 13. 6% (2011/14757)and 48.1% (1161/2415) for paid donors and plasma pheresis donors respectively. It was also found that the anti-HCV-positive rates of paid donors from Guan and Yongqing counties were (16.4%82/499) and 13.9% (53/381) respectively, 50% (575/1148) and 56% (540/965) for plasma pheresis donors from those counties respectively. The positive rates increased with the level of ALT. More than 45.5% donors whose ALT was above 45 U had anti-HCV.

The necessity and fundamental practice of external quality assesment on testing sera for HBsAg in nationwide blood bank system, and the progress and existing problems of the assesment were briefly described.

82 serum samples from the blood donors were comparatively tested by using different reagent kits and two methods (enzyme-linked immunosorbent assay, ELISA anl passive hemagglutination inhibition assay, PHA). The results are as follows: the positive rates of A1, A2 , A3 , A4-1 and B (containing mixed antigens) are 56.09%, 71.95%, 47.56%, 63.41% and 59.75% respectively; and the positive rates of A4-2 and A4-3 (containing single antigens) are 41.46% and 62.19% respectively. The results of testing 8 of all the serum samples by four-gene-recombinent immunoblot assay (RIBA) confirmed that the results of A1, A2, A3, A4-1 and A4-3 were consistent with them. The 76th sample with positive result when tested by RIBA had a negative result when tested by RIBA, while the 82th sample with negative result when tested by RIBA had posi tive one when tested by B. It is suggested that reagent kits made in China left much to be desired in antigen-matching, compactness and cut-off value determining, though they could screen out most of the donors with positive anti-HCV results.

The effect of plasma exchange (PE) in vivo was studied. The significant reductions of Cl~-, albumin, globulin, IgG, CIC and fibrinogen were observed after plasmapheresis, but Na~+, K~+, Ca~(++), Mg~(++), Cr, BUN and C3 hadn't significant changes, while the percentage of T suppressor cells increased after PE. The possibility of thrombosis should be awared, because hypercoagulation might be induced by mechanical depletion of antithrombin Ⅲ.

The adoptation of PCR-Sequence specific primers (SSP) for HLA-DQB"medium resolution" typing was reported. This method is rapid,easy,acourete and can be used for DQB genetic typing, is useful tool for matching of organ transplantation.

Objective To study the feasibility of preoperative acute hypervolemic hemodilution for decreasing perioperative blood use. Methods Thirty two patients(ASAI-II) undergoing spinal surgery were randomized into AHH group and control group. After anesthesia 6% HES(200/0.5) was given through internal jugular vein for AHH. The blood volume was increased by about 25%. During the operation, blood loss was replaced by HES in equal volume. Blood was transfused when the Hct dropped below 25%. The HR, MAP, CVP, SPO2, Hb, Hct., Plt, FIB, APTT, PT, amount of blood loss, blood transfused and urine output, volume of drainage 24h after operation and VAS score (0 means no effect, 10 means maximal effect) were observed before AHH, at the end of AHH, at the end of operation, and 24h after the operation. Blood volume expansion was calculated. Results (1) HR increased significantly in both groups(P

Objective A rare HLA phenotype carrying three HLA-A antigens, A2, A11.2 and A24, was identified in a potential bone marrow donor SZHD1. Methods To determine molecular basis of this phenotype the HLA-A gene fragments from this donor and his family members were cloned and sequenced. Results Sequencing analysis indicated that the donor carries an unusual HLA haplotype, A*1102, A*24020101; B*38; DRB1*15. Conclusions Other four family members were found to carry this haplotype, which as a Mendelian gene was segregated and stably transmitted through three generations. This is a first example of a family carrying triple HLA-A antigens to the best of our knowledge.