The frequencies of HLA-DR and DQ were investigated in Shanghai Crowd of Han Nationality with the whole typing serum of the first in- ternational red cross HLA workshop. The frequencies of HLA-DR split and HLA-DQ split were first described in Chinese. The result. the gene frequencies of HLA-DR. DR1 0.0054, DR4 0.0906, DR7 0.0847, DRw8 0.0499, DR9 0.1553, DRw10 0.0109,DRw11 0.0847,DRw12 0.1086,DRw13 0.0906,DRw14 0.0219,DRw15 0.1332,DRw16 0. 0191,DRw17 0.0556, DR blank 0.0895, the gene frequencies of HLA-D: DQw2 0.1426, DQw4 0.0527, DQw5 0.0642,DQw6 0.2393, DQw7 0.2324, DQw8 0.0219, DQw9 0.1521, DQ blank 0.0946.

Estimation of the storage time of human blood superoxide dismutase (hSOD) in two preparation forms (freeze-dried and soluble states) at room temperature by the heating and accelerating experiment was reported. The freeze-dried hSOD had kept its 90% activity for as long as 3 years, while the soluble hSOD could have preserved its 90％ activity for only 20 hours. The methodhad proved to be relatively reliable when used for many series of specimens.

The quantitative determination of octanoate content in albumin product was performed by acid base titration directly after the water phase was mixed up with the ether phase using the mixture solvent solution. The three factor optimum seeking principle was used to seek the optimum concentrations and volumes of components in mixture solvent solution and the optimum conditions of extraction. The method had its recovery rate of 99. 8+0. 89%(n=6) and variation coeff-cient (CV)of 0. 89%(n= 16),indicating that the method was a precise and reliable one.

Objective To understand the gene characteristics of envelope region of human T lymphotropic virus type Ⅰ(HTLV Ⅰ)from Shantou region of China. Methods The gene of proviral envelope region of HTLV Ⅰ Shantou strain (named HTLV Ⅰ WHP)was amplified by PCR,using 4 designed primers,and then was sequenced,meanwhile it was compared with that of various HTLV Ⅰ representative strains from all around the world,and subsequently it was analyzed to construct its phylogenetic tree.Results The nucleotide sequence of complete envelope region of HTLV Ⅰ WHP provirus we have obtained was 1466 bp.There no deletion and insertion in the deducted amino acid sequence.The results of phylogenetic tree analysis showed that the HTLV Ⅰ WHP strain was most closed to some of the strains from Japan and Caribbean,and like the latter,belonged to the subtypea of cosmopolitan group.Conclusion The HTLV Ⅰ strains either from the coastal region of Southeast China or from Japan,Caribbean and so on have the common origin of evolution.

Objective To induce CD4+CD25+ regulatory T lymphocytes from mononuclear cells of rhG-CSF mobilized peripheral blood,and to analyze the phenotype and function of them.Methods Blood samples of ten donors of peripheral blood stem cell were collected,CD4+CD25–T lymphocytes from rhG-CSF mobilized peripheral blood(G-PB group) and unmobilezed peripheral blood(PB group) were isolated with a CD4+CD25+T lymphocytes selection kit,and then were induced by TGF-?1.FCM,RT-PCR,cell proliferration and suppression assay were used to test the expression of CD25+,Foxp3 and suppressive function,and the difference of the rate and the suppressive activity of CD4+CD25+T cells were compared.Results 1) After treated with anti-CD3mAb and TGF-?1,there was a difference between CD4+CD25–T lymphocytes isolated from G-PB group and PB group,the expression of CD25+ were(77.9?2.3)% and(65.7?4.2)%,respectively(P

Red cell concentrates (Hct≥90%) weresuspended in SAGS medium(RCS.contain-ing sodium chloride,adenine,glucose,sucrose),and in H(?)gman's SAGM me-dium (containing sodium chloride,adenine,glucose,mannitol).Both were stored at4?2℃ for 35 days and compared withbiochemical tests.The results of posttrans-fusion survival,ATP,2,3-DPG,pH,K~+ tests between the two preparationswere similar,and the mean values ofinvitro hemolysis of SAGS-RCS was signi-ficantly lower than that of SAGM-RCS,i。e.1.71?0.95g/L and 3.10?0.19g/L res-pectively (P

The short chain fatty acids inplatelet concentrate (PC) and serumwere determined by means of gaschromatography.The short chainfatty acids determined includeacetic,propionic,butyric,isobuty-ric,valeric,isovaleric,lactic,pyruvic and succinic acids.Therewere acetic,lactic,pyruvic andsuceinic acids in PC;their relativecontents were 1.14,13.50,1.41 and9.54,respectively.The serum cont-ained acetic,lactic and pyruvic aci-ds;their relative contents were0.98,4.33 and 1.88,respectively.Succinic acid wasn't found in theserum,suggesting that the succinicacid in PC is the content of platelets.There was a positive correlationbetween the platelet count and lacticacid content of PC.Fresh PC wascompared with the PC of same volumepreserved for 0,24,48,72 hours atroom temperature.The lactic acidcontent of PC increased with thetime of preservation.

A case of the 12-year-old female patient with first relapsed acute lymphoblastic leukemia was reported. During the induction chemotherapy, the patient, transfused with whole blood and granulocytes, developed diarrhea and generalized macular rash. The skin biopsy showed some changes compatible with graft versushost disease (GVHD). Therefore, the related literature was reviewed, and the clinical features of transfusionassociated GVHD and the measures for its prevention and treatment were succinctly introduced.

To do rapid testing for incomplete antibody in the process of blood matching, a more suitable incomplete antibody rapid agglutinant(IRAT) and a neutralizer used in combination with IRAT were developed. The former consisted of protamine, dextran, albumin and EDTA, while the latter consisted of sodium citrate, albumin and NaCl. The method of using the agglutinant and neutralizer in testing for incomplete antibody was briefly called as IRAT method. It took 3 minutes for the method to give test result. IRAT method could be used in blood matching blood grouping aud in testing the IgG for anti-A and anti-B. Preliminary comparative observations of testing for incomplete antibody in patients with AIHA by IRTA and immediate Coombs test gave basically consistent results (Original article on page 9)

9.9Log10 bacteriophage R 17 Conclusion Alkylation of the phenothiazine ring increased the potential of inactivating virus and decreased the damage to red blood cells? M007 might be an alternative phenothiazine dye that can be used to inactivate viruses in red blood cells?