The frequencies of HLA-DR and DQ were investigated in Shanghai Crowd of Han Nationality with the whole typing serum of the first in- ternational red cross HLA workshop. The frequencies of HLA-DR split and HLA-DQ split were first described in Chinese. The result. the gene frequencies of HLA-DR. DR1 0.0054, DR4 0.0906, DR7 0.0847, DRw8 0.0499, DR9 0.1553, DRw10 0.0109,DRw11 0.0847,DRw12 0.1086,DRw13 0.0906,DRw14 0.0219,DRw15 0.1332,DRw16 0. 0191,DRw17 0.0556, DR blank 0.0895, the gene frequencies of HLA-D: DQw2 0.1426, DQw4 0.0527, DQw5 0.0642,DQw6 0.2393, DQw7 0.2324, DQw8 0.0219, DQw9 0.1521, DQ blank 0.0946.

Estimation of the storage time of human blood superoxide dismutase (hSOD) in two preparation forms (freeze-dried and soluble states) at room temperature by the heating and accelerating experiment was reported. The freeze-dried hSOD had kept its 90% activity for as long as 3 years, while the soluble hSOD could have preserved its 90％ activity for only 20 hours. The methodhad proved to be relatively reliable when used for many series of specimens.

The quantitative determination of octanoate content in albumin product was performed by acid base titration directly after the water phase was mixed up with the ether phase using the mixture solvent solution. The three factor optimum seeking principle was used to seek the optimum concentrations and volumes of components in mixture solvent solution and the optimum conditions of extraction. The method had its recovery rate of 99. 8+0. 89%(n=6) and variation coeff-cient (CV)of 0. 89%(n= 16),indicating that the method was a precise and reliable one.

200 units of eligible blood from donors with negative HBsAg reaction had been tested for hepatitis B virus markers (HBVM), using ELISA and dot-blot hybridization. The results of testing showed that 120 of the 200 units of blood had negative HBVM reaction, and the rest 80 units of blood had positive HBVM reaction. Among these 80 units of blood, 62 units had negative hepatitis B virus replication markers (HBVRM) and the rest 18 units had positive HBVRM. The rate, of post-transfusion HBV infection in the patients received HBVM (+) blood was 25.4%, significantly higher than the rate of post-transfusion HBV infection (3.75%) in the patients received HBVM (-) blood (P

A Human blood type antigen P1 with very high activity was separated from sheep hydatid cyst fluid. Determination of the antigen by ultraviolet spectrum, polyacrylamide electrophoresis, gel filtration, and determination of its carbohydrate content and aminoacid residue had indicated that the substance was a glycoprotein, its total carbohydrate content was 80％ about one-fifth of which was hexosamine, and other carbohydrate components were glucqse, galactose and glucosamine. Its protein consisted of 14 aminoacids, and its molecular weight was about 30000 Daltons. By immunizing the animals with this substance, the anti-P1 serum of high titer could be obtained. The successful extraction of this substance P1 with high activity would provide the abundant resource for reseach on human blood type antigen and its immunochemistry, and create the good conditions for studies in preparation and application of anti-P1 serum or monoclonal antibody toward P1.

A success in reconstruction of the hematopoietic function of marrow in a case of malignant lymphoma attained by autologous peripheral blood stem cell transplantation after the ultra-high dose chemoradiotherapy was reported. The platelet count of patient was increased to over 20 ? 109/L at the eighth day after PBSC transplantation and was recovered to over 50?109/L at the tenth day after PBSC transplantation. The white blood cell count was increased to over 0. 5?109/ L at the ninth day after PBSC transplantation. The pelvic CT-graphy showed reduction in size of the tumor. And then the patient was outhospitalized due to remission,having been surviving for more than 6 months with routine follow-up. It was suggested that the autologous peripheral blood stem cell transplantation could be a safe and effective suplement therapy for radical cure of malignant tumor.

The sample treatment withRivanol-Ferrous ammumium sulfateis a new procedure which we searchedfor in analyzing Tf C subtypes amongnationalities of South China.Thesample subjected to this treatmentand isoelectric focusing gave clearlyvisible bands,reliable typing resul-ts,and good reproducibility.Onecould determine directly Tf C sub-types and their variants without im-munofixation.The pracedure is easyto perform,economically practical,and can be used in the anthropolongy,population genetics analysis,and inthe TF individual identificationand parent-child relation testing inthe forensic science.

In the paper, 32 lots of intravenous immunoglobulin (IVIG)were tested by an animal model,the hypotensive test of rats. A linear correlation was found between prekallikrein activator (PKA)activity and hypotensive reaction if the inflow of human IVIG was made rapid (r=0.96,n=23). The preliminary observations indicated that it would be satisfactory that the PKA activity was below 35IU/ ml and the size of reduction in blood pressure was not 15% higher than that of control animal's level.The rat model can provide a method for safe usage of the intravenous immunoglobulin and reliable detection of and control over the residual PKA activity in blood products.

A comparison between 70-blood-donor and 70-health-control blood lipoids,blood composition,and hemorrheological indexes by paired analysis method basedon occupation,sex and age was made.Paired T-test showed that though severalindexes of the blood donors reduced remarkably,they were still within the normalphysiological limits,There were no abnormal findings through physical examinationand ECG,indication that according to the general physique and present diet condi-tions of Chinese population.It is safe to donate 300ml blood once at intervals of three months.

A safe and effective method for separating albumin from autoplasma by heatwas introduced.Immunological analysis,showed that such an albumin solutionhad the same identity as human serum albumin.Determination by acetate cellu-lose electrophoresis showed the purity of the albumin was over 90%.Determination by polyacrylamide gel electrophoresis showed that one of thealbumin monomers was over 97%.Detection by double radial immunodiffusionindicated that there were no any new antigens in it.The recovery of the albuminwas more than 80%.