1.Effect of Yangqing Chenfei Formula on epithelial-mesenchymal transition in silicon dioxide-induced silicosis rats
WANG Xiang cheng LI Jian sheng TIAN Yan ge
China Occupational Medicine 2022;49(06):601-609
Objective Yangqing Chenfei Formula -
To investigate the effect of (YCF) on epithelial mesenchymal transition (EMT)
Methods
in lung tissues of silicosis model rats. Specific pathogen free adult male SD rats were randomly divided into control
group, model group, tetrandrine group and YCF group, with eight rats in each group. The rats in the model group, tetrandrine
group and YCF group were intratracheally injected with 1.00 mL of silica suspension with a mass concentration of 50.0 g/L, and
the rats in the control group were given an equal volume of 0.9% sodium chloride solution. On the 15th day after modeling, the
tetrandrine group was given tetrandrine at a dose of 27.0 mg/kg body weight, the YCF group was given YCF with a dose of 8.91 g/kg
body weight, while both the control group and model group were given 2.00 mL 0.9% sodium chloride solution. Gavage wasperformed twice a day in the morning and evening for 14 days. On day 29 of the experiment, after evaluating the tidal volume,
-
functional residual volume (FRC) and vital capacity of rats in each group, lung tissues were collected, and hematoxylin eosin
staining and Masson staining were performed to examine the histopathological changes, and the fibrosis score was evaluated.
- -
Hydroxyproline level was detected by colorimetry. The expression of type Ⅰ collagen (COL Ⅰ), type Ⅲ collagen (COL Ⅲ),
- - - - - -
E cadherin (E Cad), N cadherin (N Cad) and α smooth muscle actin (α SMA) protein was detected by immunohistochemistry.
-
The expression of epithelial cell adhesion molecule (EpCAM) and fibroblast specific protein 1 (FSP 1) was detected by
Results
immunofluorescence. The lung structure was intact and the alveolar structure was normal in the control group. The
alveolar structure was destroyed, the alveolar wall was thickened, and cellular nodules were observed/n the model group. The
lung tissue lesions of rats in the tetrandrine group and YCF group were reduced compared with that in the model group, and there
was no difference in the degree of lesions between the two groups. The tidal volume, FRC and vital capacity of rats in model
P< - P<
group decreased (all 0.05), the relative expression of E Cad protein in lung tissue decreased ( 0.05), the fibrosis score and
- - - -
the level of hydroxyproline, the protein relative expression of COL Ⅰ, COL Ⅲ, N Cad and α SMA in lung tissue increased (all
P< -
0.05), while the fluorescence intensity of EpCAM protein decreased, and that of FSP 1 protein increased compared with the
P<
control group. The tidal volume, FRC and vital capacity of rats in tetrandrine and YCF groups increased (all 0.05), the fibrosis
- - -
score and the level of hydroxyproline, the protein relative expression of COL Ⅰ, N Cad and α SMA in lung tissue decreased (all
P< - P<
0.05), the relative expression of E Cad protein in lung tissues increased ( 0.05), while the EpCAM protein fluorescence
-
intensity increased and FSP 1 protein fluorescence intensity decreased compared with the model group. The relative expression
- P< Conclusion
of N Cad protein in lung tissues of YCF group was lower than that of the tetrandrine group ( 0.05). YCF can
-
improve the lung function, alleviate collagen deposition in lung tissues, and inhibit the epithelial mesenchymal transition in
silicosis model rats, and then attenuates the progression of silicotic fibrosis.
2.Effect of psychological capital and social support on job satisfaction among workers in a natural gas field
WU Hui GU Gui zhen LI Fu ran ZHOU Wen hui SHAO Jian zhong YU Shan fa
China Occupational Medicine 2022;49(06):610-614
To explore the effects of psychological capital social support and their interaction on job satisfaction in
Methods
natural gas field workers. A total of 1 473 workers from a natural gas field were selected as the research subjects
,
using convenient sampling method. Job Satisfaction Questionnaire Psychological Capital Questionnaire and Social Support
, Results
Scale were used to investigate the scores of job satisfaction psychological capital and social support level. The
, ,
detection rates of job satisfaction psychological capital and social support in the high level group were 55.9% 52.5% and
,
48.1% respectively. The detection rates of job satisfaction of workers in the high level psychological capital group and high level
(
social support group were higher than those in the low level psychological capital group and low level social support group 67.4%
vs , vs , P )
43.3% 71.9% 41.2% all <0.01 . The results of multivariate logistic regression analysis showed that psychological capital
[ (CI) ( - )
and social support had positive effects on job satisfaction odds ratio and 95% confidence interval were 1.58 1.17 2.41
( - ), , P ] ,
and 2.53 1.82 3.52 respectively all <0.01 . Moreover the psychological capital and social support had additive effect on
[ CI ( - ), CI
job satisfaction relative excess risk of interaction and 95% was 3.07 1.02 5.12 attributable proportion and 95% was
( - ), CI ( - )],
0.48 0.35 0.61 synergy index and 95% was 2.34 1.72 3.16 but there was no multiplication interaction between
(P )Conclusion
psychological capital and social support >0.05 . Psychological capital and social support can positively affect
job satisfaction of natural gas workers. There is an additive interaction between psychological capital and social support on job
,
satisfaction but no multiplicative interaction is found.
Keywords: ; ; ; ; ;
Psychological capital Social support Job satisfaction Interaction Natural gas Worker
3.Relationship of urine cobalt and individual exposure levels of cobalt and its compounds in cobalt exposed workers
LUO Xiao ting CHEN Wen DONG Ming WU Chuan LIN Zuo kan TAN Jian ming WU Bang hua
China Occupational Medicine 2022;49(06):615-620
Objective -
To analyze the relationship between cobalt level of post shift urine and individual exposure level of
,
cobalt and its compounds in cobalt exposed workers and to explore the feasibility of using urine cobalt as a biomarker.
Methods -
A total of 148 occupational cobalt exposed workers from a new material company were selected as the exposed
, - -
group and 44 non occupational cobalt exposed workers from the company were selected as the control group using the typical
sampling method. The exposure concentration of time weighted average of cobalt and its compounds in the workplace air of the
-
two groups was determined by inductively coupled plasma mass spectrometry as the individual exposure level. The cobalt levels
- -
of pre shift and post shift urinary samples of the two groups were detected by this method. The linear relationship between the
-
cobalt level of post shift urine and the individual exposure level of cobalt and its compounds in the air of the workplace was
Results - 3
analyzed. The individual exposure level of cobalt and its compounds in the exposed group was 1.10 131.71 μg/m with
(M) 3
the median of 12.23 μg/m. No cobalt and its compounds were detected in the workplace air in the control group. The cobalt
- -
levels of pre shift and post shift urines in exposed group were higher than those in the control group at the same time point
(M: vs , vs , P ) - -
1.54 0.56 μg/L 8.77 0.83 μg/L all <0.01 . The cobalt level of post shift urine was higher than that in pre shift
(M: vs ,P ),
urine in the exposed group 8.77 1.54 μg/L <0.01 and it was positively correlated with the individual exposure level
( ,P ) ,
of cobalt and its compounds Spearman correlation coefficient=0.86 <0.01 . After common logarithm conversion the linear regression equation of the cobalt level of post shift urine and the common logarithm of individual exposure level of cobalt and
(x) :ŷ x( ;F ,
its compounds in the exposed group was as follows = −0.178 + 0.988 coefficient of determination=0.72 =374.75
P ;t , P ) Conclusion -
<0.01 = - 19.36 <0.01 . There was a linear correlation between cobalt level of post shift urine and
occupational cobalt exposure level of cobalt exposed workers. Urinary cobalt can be used as a biomarker of occupational cobalt
4.Association between urinary polycyclic aromatic hydrocarbons metabolites and high-normal blood pressure in coke oven workers
MA Yi fei YAO Xi yuan WANG Hui min FU Meng meng WANG Yi dong NIE Ji sheng
China Occupational Medicine 2022;49(06):621-625
Objective ( )
To investigate the association between urinary polycyclic aromatic hydrocarbons PAHs metabolites
- Methods
and high normal blood pressure in coke oven workers. A total of 433 coke oven workers were selected as the study
-
subjects using convenient sampling method. They were divided into normal blood pressure group and high normal blood pressure
group according to their blood pressure level. The levels of ten kinds of urinary hydroxylated PAHs metabolites were measured by
- Results -
high performance liquid chromatography mass spectrometry. Among the subjects,57.5% had high normal blood
- , - , -
pressure. The levels of 1 hydroxynathalene 2 hydroxyphenanthrene 1 hydroxyphenanthrene and the metabolite of total PAHs
- ( P )
in the high normal blood pressure group were higher than those in the normal blood pressure group all <0.05 . The results of
- , - , - ,
the multivariate logistic regression analysis showed that urinary 1 hydroxynathalene 2 hydroxyfluorene 3 hydroxychrysene
- ( P ),
and metabolite of total PAHs were all risk factors for high normal blood pressure in coke oven workers all <0.05 after
, , , , ,
adjusting for confounding factors such as gender length of service body mass index smoking index alcohol consumption tea
, , , Conclusion
consumption night shift exercise frequency and other PAHs metabolites. Exposure to PAHs in coke oven
plants may increase the risk of elevated blood pressure within the normal range among coke oven workers.
5.Comparison of the application of different version of diagnostic criteria for occupational noise-induced deafness
ZHANG Xiao xiao YANG Ai chu LIANG Xiao yang XIAO Ming hui CAO Dan yan
China Occupational Medicine 2022;49(06):626-630
Objective - ( )
To explore the influence on the diagnosis of occupational noise induced deafness ONID using three
, Methods
versions of diagnostic criteria in 2002 2007 and 2014. A total of 1 766 workers who asked for ONID diagnosis
were selected as the research subjects using judgment sampling method. The results of pure tone audiometry were collected.
GBZ 49-2002Diagnostic Criteria of Occupational Noise-inducedHearing Loss(
The ONID was diagnosed using hereinafter referred to as
GBZ 49-2002),GBZ 49-2007Diagnostic Criteria of Occupational Noise-induced Deafness( GBZ 49-2007)
hereinafter referred to as
GBZ 49-2014 Diagnostic of Occupational Noise-induced Deafness( GBZ 49-2014),
and hereinafter referred to as and the
Results - - , -
diagnostic results were compared. Compared with GBZ 49 2002 and GBZ 49 2007 diagnosis with GBZ 49 2014 had
( vs , vs , P ), ( vs ,
a higher rate of ONID 57.9% 66.0% 44.8% 66.0% both <0.01 and had a higher rate of mild ONID 47.3% 54.6%
vs , P ) - -
36.0% 54.6% both <0.01 . The diagnostic rate for ONID using GBZ 492014 was higher than those using GBZ 49 2002 and
- ( P )Conclusion -
GBZ 49 2007 in each age groups all <0.01 . GBZ 49 2014 improved the diagnostic rate of ONID compared
- -
with GBZ 49 2002 and GBZ 49 2007. The reason is related to the inclusion of 4 000 Hz hearing threshold with a weight of 0.1
-
as the diagnostic hearing threshold and the use of a new age and gender correction method in GBZ 49 2014.
6.Analysis on disease burden of occupational coal workers′ pneumoconiosis in Anhui Province
HU Qiong CHEN Bao chun HU Xun jia XIE Qiao mei ZHA Wan sheng SONG Yuan chao
China Occupational Medicine 2022;49(06):635-639
Objective
To analyze the disease burden caused by occupational coal workers' pneumoconiosis (CWP) in Anhui
Methods
Province. A total of 1 649 patients with occupational CWP diagnosed in Anhui Province from 1950 to 2019 were
selected as the study subjects using a retrospective study method. Age, gender, survival time, location, working age of disease
onset, age of death, stage and date of diagnosis of CWP, comorbidities at the time of investigation, hospitalization and outpatient
expenses in the past year, cost of absence from work, cost of absence from work of caregivers, and cost of nutrition and
transportation were investigated. The years of life lost, years lived with disability (YLDs), disability adjusted life years (DALYs)
Results
and economic losses were calculated. Among these patients, 1 405 cases survived and 244 cases died. In the age range
- - -
of 20.0 70.0 years, the YLDs of surviving patients were 2.12 22.20 (8.19±3.59) person years. The YLDs of patients with disease
vs - P
complications was higher than that of patients without complications [(8.55±3.95) (7.87±3.21) person years, <0.01]. The total
- -
DALYs of the patients was 14 031.59 person years, and the average per capita DALYs was 8.51 person years. Its YLDs
accounted for 82.0 % of the total DALYs. The total economic loss caused by CWP in the 1 405 surviving patients was 354.903 0
Conclusion
million yuan, and the average per capita economic loss was 252 600 yuan. The disease burden caused by CWP is
relatively high in Anhui Province. In addition to early detection, diagnosis and treatment, it is necessary to focus on prevention
and treatment of CWP complications to reduce the disease burden.
7.Changes of renin-angiotensin-aldosterone system in workers exposed to noise
WU Qi feng LI Qi ping LI Cong LIANG Wei hui LI Bin LI Wan li DENG Xiao feng
China Occupational Medicine 2022;49(06):640-644
Objective - ( )- ( )
To observe the effects of renin angiotensin Ang aldosterone system RAAS in workers exposed to
Methods - -
occupational noise. Forty five workers with suspected occupational noise induced deafness were selected as noise
, ,
exposure group using convenient sampling method. According to their tinnitus symptom noise exposure intensity and work age
- , ,
they were divided into no tinnitus and tinnitus subgroups <90 dB and ≥90 dB subgroups work years <10 years and ≥10 years
subgroups. Another 45 workers with no occupational noise exposure history were selected as control group. The levels of plasma
( ), , ,
renin activity PRA AngⅠ AngⅡ and aldosterone of the two groups were detected and the aldosterone to renin activity
Results
ratio was calculated. The diastolic blood pressure of the noise exposure group was higher than that of the control group
[( )vs( ) ,P ] ,
80±7 76±8 mmHg <0.05 . However there was no significant difference in systolic blood pressure between the two
(P ) ( :
groups >0.05 . The level of plasma AngⅡ in the noise exposure group was higher than that in the control group median
vs ,P ) ( P )
100.98 65.43 μg/L <0.05 . There was no statistical significance in other indexes between the two groups all >0.05 . The
( :
plasma AngⅡ level in < 90 dB subgroup in the noise exposure group was higher than that of the control group median 123.16
vs ,P )
65.43 μg/L <0.05 . There was no statistical significance in other indexes among the two subgroups of tinnitus symptom or
( P )
work age in the noise exposure group and the control group all >0.05 . There were no significant differences in the abnormal
, ( P )
rates of PRA AngⅡ and aldosterone in plasma between the noise exposure group and the control group all >0.05 .
Conclusion
Occupational noise exposure may affect RAAS and lead to increased plasma AngⅡ levels in the workers.
-
Tinnitus and work age may not affect RAAS in occupational noise exposure workers.
8.Changes of renin-angiotensin-aldosterone system in workers exposed to noise
WU Qi feng LI Qi ping LI Cong LIANG Wei hui LI Bin LI Wan li DENG Xiao feng
China Occupational Medicine 2022;49(06):640-644
Objective - ( )- ( )
To observe the effects of renin angiotensin Ang aldosterone system RAAS in workers exposed to
Methods - -
occupational noise. Forty five workers with suspected occupational noise induced deafness were selected as noise
, ,
exposure group using convenient sampling method. According to their tinnitus symptom noise exposure intensity and work age
- , ,
they were divided into no tinnitus and tinnitus subgroups <90 dB and ≥90 dB subgroups work years <10 years and ≥10 years
subgroups. Another 45 workers with no occupational noise exposure history were selected as control group. The levels of plasma
( ), , ,
renin activity PRA AngⅠ AngⅡ and aldosterone of the two groups were detected and the aldosterone to renin activity
Results
ratio was calculated. The diastolic blood pressure of the noise exposure group was higher than that of the control group
[( )vs( ) ,P ] ,
80±7 76±8 mmHg <0.05 . However there was no significant difference in systolic blood pressure between the two
(P ) ( :
groups >0.05 . The level of plasma AngⅡ in the noise exposure group was higher than that in the control group median
vs ,P ) ( P )
100.98 65.43 μg/L <0.05 . There was no statistical significance in other indexes between the two groups all >0.05 . The
( :
plasma AngⅡ level in < 90 dB subgroup in the noise exposure group was higher than that of the control group median 123.16
vs ,P )
65.43 μg/L <0.05 . There was no statistical significance in other indexes among the two subgroups of tinnitus symptom or
( P )
work age in the noise exposure group and the control group all >0.05 . There were no significant differences in the abnormal
, ( P )
rates of PRA AngⅡ and aldosterone in plasma between the noise exposure group and the control group all >0.05 .
Conclusion
Occupational noise exposure may affect RAAS and lead to increased plasma AngⅡ levels in the workers.
-
Tinnitus and work age may not affect RAAS in occupational noise exposure workers.
9.Evaluation on the implementation of HealthWISE in a grade-A tertiary hospital
China Occupational Medicine 2022;49(06):645-651
Objective
To evaluate the implementation effect of work improvement in health services technical tool
( ) -
HealthWISE developed by the International Labor Organization and the World Health Organization in a grade A tertiary
Methods -
hospital. A total of 14 healthcare workers who had participated in the HealthWISE teacher training in a grade A
-
tertiary hospital since 2016 were selected as the research subjects using a typical sampling method. Semi structured interviews
, Results ,
were conducted and the interview data were collected and analyzed. Among the 14 subjects five participated in the
training for more than three times. The research subjects believed that they had gained great insights through the training. The
,
training had led to positive changes in both individual and team levels. In particular the hospital had established an
, ,
occupational health protection system which had been significantly improved in organizational construction training and
, - ,
education capacity building and so on. During the prevention and control of the COVID 19 pandemic the hospital strengthened
HealthWISE application to ensure the occupational safety and health of healthcare workers. All research subjects provided
opinions and suggestions on the improvement of the national comprehensive occupational health protection system for healthcare
Conclusion ,
workers in the future. The application of HealthWISE in this hospital has achieved remarkable results which
helps to promote the establishment of a comprehensive occupational health protection system for healthcare workers on a large
scale.
10.Effects of occupational mercury exposure on the immune function of workers
China Occupational Medicine 2022;49(06):657-661
Objective
To investigate the effects of mercury on T lymphocytes and serum immune indexes of workers with
Methods
occupational mercury exposure. A total of 45 workers with occupational mercury exposure were selected as the
,
mercury exposure group and 47 workers without occupational mercury exposure were selected as the control group using the
judgment sampling method. Cold atomic absorption spectrometry was used to detect the urinary mercury level of the two groups.
( ) +, + +, + + - +
Flow cytometry was used to detect the proportion of cluster of differentiation CD 3 CD3CD4 CD3CD8 and CD3CD19
, - ( - ) - ( - )
cells in peripheral blood and the levels of tumor necrosis factor α TNF α and interleukin 8 IL 8 in serum. The levels of
( ) , Results
immunoglobulin Ig A IgG and IgM in serum were measured by immune nephelometry. The urinary mercury level of
( : vs ,P )
individuals in the mercury exposed group was higher than that of the control group median 92.7 13.2 μg/g Cr <0.01 . The
+, + +, - +
proportion of CD3 CD3CD4 CD3CD19 cells in peripheral blood and serum IgG level in the mercury exposed group
( P ), - - ( P )
decreased all <0.05 and the serum TNF α and IL 8 levels increased all <0.01 compared with the control group. Urinary
-
+
mercury level was negatively correlated with the proportion of CD3CD19 cells in peripheral blood and serum IgG level in the
[ (r) , , P ],
study subjects Spearman correlation coefficient S were −0.21 and −0.31 respectively all <0.05 and positively
- - (r , , P ) ,
correlated with serum TNF α and IL 8 levels S were 0.36 and 0.39 respectively all <0.05 . However the urinary mercury
( P ), +, + +,
level was neither correlated with IgA and IgM levels in serum all >0.05 nor with the proportion of CD3 CD3CD4
+ + ( P ) Conclusion
CD3CD8 cells in peripheral blood all >0.05 . Occupational exposure to mercury can lead to abnormal
,
changes in peripheral blood T lymphocyte subsets B lymphocytes and serum immune factors in workers. The mercury load of occupational mercury exposure workers may impact their immune function.