Objective To explore the expressions and significance of NF-κB p65 in different place endometriosis.Methods A total of 48 specimans with endometriosis (16 cases of extrapelvic endometriosis,32 cases of ovarian en-dometriosis and meanwhile 22 cases of eutopic endometrium) were enrolled in the study,20 cases of normal en-dometrium were involed as control group. Immunoh istochemistry was used to detect the expressions of NF-κB p65 in above groups. Results The expressions of NF-κB p65 were found in the glandular epithelial cells in all groups. The ex-trapelvic endometriosis cases showed the highest expression,followed by ectopic endometrium, eutopic endometrium,and showing the lowest in normal endometrium,the difference among those groups was significant (P<0.05). However,the difference between prolificative phase and secretory phase was not significant (P>0.05). Furthermore, there was no correlation with clinical stages in ovarian endometriosis. Conclusion NF-κB p65 proteins are highly expressed in extrapelvic and ectopic endometrium,which suggests that they may participate in the development and progression of endometriosis.

Objective Using hybridoma technique and screened hybridoma cell strains stably, efficiently secreted anti glycosylated hemoglobin monoclonal antibody to provide specific material for the development of glycosylated hemoglobin ELISA kit. Methods The immune antigen was prepared by maleimide method, multi-level immune mice by BALB/c,through cell culture fusion, screening of hybridoma cell culture medium HAT, ammonium sulfate salting out method and G protein chromatography. Monoclonal antibody subclasses were identified by monoclonal antibody subtype identi-fication Kit operation. Results Through cell fusion, screening and cloning culture, etc., the final selection screened 1 strain stably secreting specific antibody hybridoma cell line, named N5B4; cell culture supernatant liquid was 1:5000, ascites titer was 1:100 million; a standard curve to calculate the concentration in the sample human glycat ed hemoglobin was 99.2%. Conclusion After KET monoclonal antibody cell line to obtain a high specificity and high sen-sitivity of screening.

Objective To investigate the expression of the connective tissue growth factor (CTGF) and integrin β1 in vaginal anterior wall of the model rats with stress urinary incontinence. Methods Twenty-four healthy female SD rats were randomly divided into three groups, group A rats as control group without any treatment;group B rats only with vaginal expansion;group C rats with vaginal expansion and bilateral ovaries removed. At postoperative four weeks to determine the maximum bladder capacity (MBC)and abdominal pressure leakage point pressure (ALPP), to establish detection model for stress urinary incontinence. Modeling successfully executed in rats after four weeks after vaginal anterior wall tissue,immunohistochemical staining method and real-time fluorescent quantitative RT-PCR technique to detect the expression of CTGF and integrinβ1. Results The urine flow dynamic detection(MBC,ALPP)B and C group was significantly lower than group A, there were statistically significant differences (P<0.05);CTGF mRNA and protein expression in group C was significantly lower than group A(P<0.05),there were no statistical significance between group A and group B (P>0 . 05);integrin β1 mRNA and protein in group C expression was low er than that in group A and B(P<0.05), and differences between A and B were statistically significant(P<0.05). Conclusion The anterior wall vaginal CTGF in rats in SUI and low expression of integrin β1 may be related to stress urinary incontinence and other pelvic floor support structure there is a close relationship of the occurrence of degenerative diseases of the pelvic floor support degenerative diseases.

Objective To observe the clinical effect of nasal continuous positive airway pressure (Ncpap) combined with small dose of phentolamine in treatment of elderly patients with severe pneumonia. Methods A total of 95 cases of el-derly patients with severe pneumonia in our hospital from February 2013 to January 2014 were chosen. And they were randomly divided into the control group(47 cases) and the observation group(48 cases). The control group received the routine therapy combined with Ncpap, while the observation group was given the conventional treatment combined with Ncpap and small dose phentolamine treatment. The clinical symptom improvement time, the hospitalization time, blood gas index changes, the total efficiency of treatment in two groups were compared. Results The clinical symptoms disap-peared time (pulmonary rales, shortness of breath, cyanosis, liver retraction) and hospital stay of the observation group were significantly shorter than those in control group (P<0.05); After treatment 12 h, the blood gas(PaO2, PaCO2) in patients of the two groups were improved significantly than before treatment (P<0.05);And observation group blood gas improved better than the control group ( P<0 . 05 ); The total effective rate of the observation group and the control group was 95.8%and 85.1%, the total effective rate of observation group was higher than control group (P<0.05). Con-clusion Ncpap combined with small dose phentolamine are used in treatment of elderly patients with severe pneumonia, the symptoms of the patients are improved quickly, and with shorter hospital stay, the effect is significant, worthy of promotion.

Objective To observe the clinical efficacy of valsartan combined with alprostadil in treatment of diabetic nephropathy. Methods Selected 84 cases of diabetic nephropathy patients in our hospital from January 2010 to January 2014. According to the different treatment methods they were divided into observation group and control group, each of 42 cases, two groups were treated with high quality low protein diabetic diet, and received the hypoglycemic (insulin), symptomatic treatment, anticoagulant buck. The control group was treated with alprostadil injection 10 μg with 20 mL of physiological saline bolus, once a day, for 12 weeks. On the basis of control group, the observation group was given valsartan 80 mg, 2 times daily, orally, 4 weeks for a course of treatment for 12 weeks. The changes of 24 hours urinary protein, creatinine, urea nitrogen before and after treatment and the effects of two groups after treatment were com-pared. Results The 24 hours urine protein, Cr, BUN levels before treatment had no significant difference between two groups of patients (P>0.05). After treatment, two groups of patients with Cr, BUN levels had no obvious changes with those before therapy, but of 24 hour urinary protein quantitative the observation group significantly decreased than be-fore treatment and control group after treatment, there was statistically significant difference between two groups (P<0.05). After treatment, the observation group's efficiency was 90.5%, control group was 73.8%, observation group's effi-ciency was significantly higher than the control group, there was significant difference between two groups (P<0.05). Throughout the course of treatment, the control group one case of transient palpitations, three cases of dizziness and headache, one case of cough. In the observation group two cases of cough, two cases of dizziness and headache, which were mild severity and did not affect the continued treatment. Conclusion The therapeutic effect of valsartan com-bined with alprostadil in treatment of diabetic nephropathy is accurate, can significantly improve renal function, is wor-thy of promotion and application.

Objective To explore the correlation of heart rate variability and blood pressure rhythm day and night for patients with OSAHS and sequelae of cerebral infarction. Methods A total of 364 patients with sequelae of cerebral infarction who were admitted to our hospital from October 2011 to January 2014 were selected. 178 patients complicat-ed with OSAHS were assigned to OSAHS group, and the rest of 186 patients were assigned to non-OSAHS group. 180 people with normal results of physical examination during the same period were selected as healthy control group. In-dices such as blood pressure and heart rate variability day and night in the two groups were observed and compared. Results Indices of heart rate variability and blood pressure rhythm were compared, and the indices of patients in OS-AHS group were all much higher than those of the rest two groups (P<0.01). Indices of non-OSAHS group were all much higher than those of the healthy control group (P<0.01);oxyhemoglobin saturation during the night was compared, and OSAHS group was much lower than the rest two groups (P<0.01); oxyhemoglobin saturation in non-OSAHS group was lower than that in healthy control group (P<0.01). Conclusion OSAHS and sequelae of cerebral infarction are cor-related with heart rate variability and blood pressure rhythm, which affect the prognosis and needs to be attached high importance.

Objective To investigate the early, long-term effect of the craniectomy severe traumatic brain injury of early postoperative cranial decompression angioplasty patients. Methods A total of 76 cases with severe traumatic brain in-jury patients were selected from December 2010 to December 2013 in our hospital for treatment, they were divided into two groups, 38 cases in the observation group, which were treated with decompressive craniectomy after 4 to 8 weeks cranioplasty, 38 cases in the control group and the patients were treated with craniectomy 4 to 6 months postoperative cranioplasty. The early efficacy and long-term efficacy, complications occur of two groups were compared. Results The neurological function four weeks after the forming of the observation group was lower than the control group, Barthel score, Fugl-Meyer score were higher than the control group, the difference was statistically significant (P<0.05). GCS score, GOS score, quality of life scores of 2 months and 12 months after angioplasty of the observation group were higher than the control group on the same period, the difference was statistically significant (P<0.05). The overall incidence of complications in the observation group was lower than the control group, the difference was statistically significant (P<0.05). Conclusion Early, long-term clinical results of severe brain injury early decompressive craniectomy after cranio-plasty are obvious, safe and effective, can be popularized in clinical application.

Objective To analyze the clinical value of partial nephrectomy with nephronsparing under retroperitoneal laparoscopy in the treatment of renal tumors. Methods Sixty cases of patients with renal cell carcinoma in our hospital from January 2012 to January 2014 were reviewed, among which the 34 cases treated with partial nephrectomy with nephron sparing under retroperitoneal laparoscopy were set as the study group,and the other 26 cases treated with tra-ditional surgery of open renal resection were set as the control group. Results The duration of operation time, vascular occlusion ofrenal pedicle and postoperative hospital stays as well as other aspects of patients in the study group and the control group had statistically significant differences(P<0.05). Conclusion Partial nephrectomy with nephron sparing under retroperitoneal laparoscopy is of good effect for patients with kidney tumors.

Objective To analyze the clinical effects of procedure for prolapse and hemorrhoids combined with external hemorrhoidectomy in the treatment of mixed hemorrhoid. Methods Clinical data of 108 patients with mix hemorrhoid who were admitted to our hospital from January 2012 to January 2014 were retrospectively analyzed. The 108 patients were assigned to an observation group and a control group according to the surgical procedures. 54 patients in the ob-servation group received procedure for prolapse and hemorrhoids combined with external hemorrhoidectomy, and 54 pa-tients in the control group received the conventional Milligan-Morgan operation. Each clinical index, pain conditions within 3 days after surgery and complications within 6 months after the surgery in the two groups were compared. Re-sults Surgery time, amount of bleeding during surgery, hospitalization time, and hospitalization expenses in the observa-tion group were all significantly better than those in the control group, and the differences were statistically significant (P<0.05). Pain scores in 24 h, 48 h, and 72 h after the surgery in the observation group were significantly lower than those in the control group, and the differences were statistically significant (P<0.05). In 24 h, 48 h and 72 h after the surgery, patients in the observation group had suffered less pain than those in the control group. Except for one of the patients who had complications before the surgery, total incidence of complications within 6 months after the surgery in the observation group was 18.51%, significantly lower than that of 81.50% in the control group, and the difference was statistically significant(P<0.05). Conclusion The procedure for prolapse and hemorrhoids combined with external hemor-rhoidectomy in the treatment of mix hemorrhoid has favorable effects of lower pain degree and less incidence of com-plications, and the combined procedure is suitable to be further promoted and applied clinically.

Objective To discuss the clinical results and surgical method in treatment of hypertensive intracerebral hemorrhage in basal ganglia. Methods From January 2007 to June 2013, the use of surgery treatment of hypertensive intracerebral hemorrhage in basal ganglia under direct vision assisted by the deep cold light suction in 104 cases,all the cases were done through lateral fissure-brain island approach,removing the hematoma with bipolar coagulation and hemostasis at sametime. This paper analyzed the group of patients with surgical methods and clinical efficacy. Results CT scan of intreatment group showed that there were 6 cases of secondary operation because of recurrent hemorrhage, and the hematoma-cleared rate>80% in 73 cases,50%-80% in 21 cases,<50% in 10 cases before operation within 6 hours. According to ADL classification,the 92 cases were alive after operation, good recovery in 65 cases, moderate disability in 22 cases, 5 cases of severe disability, death and give up treatment 12 cases. There was 12 cases of sec-ondary operation because of recurrent hemorrhage in control group, and the hematoma-cleared rate>80% in 16 cases,50%-80%in 23 cases,<50%in 24 cases before operation within 6 hours. According to ADL classification,the 45 cases were alive after operation in control group, good recovery in 14 cases,moderate disability in 16 cases,15 cases of se-vere disability,death and give up treatment 18 cases. There were significant differences between the two groups in op-eration time, intra-operative blood loss, the volume of blood transfusion, hematoma clearance rate, postoperative sur-vival rate and the survival quality ADL grading at half a year, and the indexes mentioned above in treatment group were better than those in control group. Conclusion Surgery treatment of hypertensive intracerebral hemorrhage in basal ganglia under direct vision by lateral fissure-brain island approach assisted by the deep cold light suction will shooter the operation time and lower the surgical injury,increase hematoma-cleared rate,it aslo has reliable hemostasis and more effective,so this method is worthy of clinical use.