1.Prevalence of Malaria in Pregnant Women in Lagos, South-West Nigeria.
Chimere O AGOMO ; Wellington A OYIBO ; Rose I ANORLU ; Philip U AGOMO
The Korean Journal of Parasitology 2009;47(2):179-183
Prevalence rates reported for malaria in pregnancy in Nigeria vary considerably. The accuracy of results of malaria diagnosis is dependent on training, experience, and motivation of the microscopist as well as the laboratory facility available. Results of training programmes on malaria microscopy have shown low levels of sensitivity and specificity of those involved in malaria diagnosis routinely and for research. This study was done to ascertain the true prevalence of malaria in pregnancy in Lagos, South-West Nigeria. A total of 1,084 pregnant women were recruited into this study. Blood smears stained with Giemsa were used for malaria diagnosis by light microscopy. Malaria infection during pregnancy presents mostly as asymptomatic infection. The prevalence of malaria in this population was 7.7% (95% confidence interval; 6.2-9.4%). Factors identified to increase the risk of malaria infection include young maternal age (< 20 years), and gravidity (primigravida). In conclusion, this study exposes the over-diagnosis of malaria in pregnancy and the need for training and retraining of laboratory staffs as well as establishing the malaria diagnosis quality assurance programme to ensure the accuracy of malaria microscopy results at all levels.
Adult
;
Blood/parasitology
;
Female
;
Humans
;
Malaria/diagnosis/*epidemiology
;
Microscopy
;
Nigeria/epidemiology
;
Pregnancy
;
Pregnancy Complications, Infectious/*epidemiology
;
Pregnant Women
;
Prevalence
;
Young Adult
2.Genetic diversity and complexity of Plasmodium falciparum infections in Lagos, Nigeria.
Muyiwa K OYEBOLA ; Emmanuel T IDOWU ; Yetunde A OLUKOSI ; Bamidele A IWALOKUN ; Chimere O AGOMO ; Olusola O AJIBAYE ; Monday TOLA ; Adetoro O OTUBANJO
Asian Pacific Journal of Tropical Biomedicine 2014;4(Suppl 1):S87-91
OBJECTIVETo analyse the genetic diversity of Plasmodium falciparum (P. falciparum) using msp-1 and msp-2 as antigenic markers.
METHODSParasite DNA was extracted from 100 blood samples collected from P. falciparum-positive patients confirmed by microscopy, and followed by PCR-genotyping targeting the msp-1 (block2) and msp-2 (block 3) allelic families.
RESULTSAll the families of msp-1 (K1, MAD20 and R033) and msp-2 (FC27 and 3D7) locus were observed. Results revealed that K1 (60/100) was the most predominant genotype of msp-1 allelic family followed by the genotypes of MAD20 (50/100) and R033 (45/100). In the msp-2 locus, FC27 genotype (62/100) showed higher frequency than 3D7 genotype (55/100). The allelic families were detected either alone or in combination with other families. However, no R033/MAD20 combination was observed. Multiplicity of infection (MOI) with msp-1 was higher in the locality of Ikorodu (1.50) than in Lekki (1.39). However, MOI with msp-2 was lower in the locality of Ikorodu (1.14) than in Lekki (1.76). There was no significant difference in the mean MOI between the two study areas (P=0.427).
CONCLUSIONSThe observation of limited diversity of malaria parasites may imply that the use of antigenic markers as genotyping tools for distinguishing recrudescence and re-infections with P. falciparum during drug trials is subjective.