The accuracy of ultrasound examination and measurement is of great significance to diagnosing ocular disea-ses.The commonly used ocular ultrasonography includes amplitude(A)-mode ultrasound,brightness(B)-mode ultra-sound,ultrasound biomicroscopy(UBM),and color Doppler flow imaging(CDFI).A-mode ultrasound is mainly used to measure the distance between tissues with different echo intensities.B-mode ultrasound can visualize the two-dimensional structure of the eye and make quantitative measurements of the target point.UBM is an ultra-high-frequency two-dimen-sional imaging method,which can clearly display the structural characteristics of the anterior segment and measure the rele-vant parameters.It can also help quantitatively analyze the morphological changes of the angle and the anterior segment be-fore and after implantable collamer lens surgery.CDFI can quantitatively measure the parameters of blood vessels by apply-ing the Doppler effect on the basis of two-dimensional ultrasound,reflecting the changes of blood flow in the eye.To standardize the operations of different ultrasound examination methods,this guideline is formulated.This guideline mainly focuses on the standardized operation of ocular ultrasound instruments and their clinical application in ocular diseases,so as to provide guidance for the diagnosis and treatment of related ocular diseases.