Burns ; Evidence-Based Medicine ; Humans

Evidence-Based Medicine ; Humans ; Resuscitation ; Shock ; therapy

Animals ; Burns ; physiopathology ; therapy ; Genetic Engineering ; Humans ; Hyperbaric Oxygenation ; Tissue Engineering ; Wound Healing

OBJECTIVETo observe the effect of Zhimu and Huangqi on increasing heart rate and cardiac hypertrophy, and its bradycardia effect, to evaluate the exercise capacity and response to stimulation and to discuss the use of Zhimu in treating heart failure.

METHOD1. Rats were treated with isoproterenol 1.25 micrograms.kg-1 i.p., and then the increasing of heart rate and the bradycardia effect were measured after administrated with Zhimu or Huangqi for 3 days. 2. Mice cardiac hypertrophy model was established by s.c. ISO 1 mg.kg-1, 2 times per day for 14 days and heart-weight-index was measured after Zhimu and Huangqi was given orally alone or jointly for 14 days. 3. Abdominal aorta banding operation was done in mice and 3 weeks after operation, they were administrated for 2 weeks, and then run-time(exercise capacity), quiet heart rate, ISO response heart rate after i.p. isoproterenol and heart-weight-index were measured. 4. Cardiac hypertrophy model (inject ISO 14 days) mice were administrated for 12 days, and the mortality and dying time of mice in cold (-20 degrees C) and heat(45 degrees C) stimulative condition were observed.

RESULTZhimu could cut down the increasing of heart rate induced by ISO, decrease significantly heart-weight-index in cardiac hypertrophy mice, reduce the quiet heart rate and prolong the run time in abdominal banding model. Zhimu combined with Huangqi could also improve the ISO response in abdominal banding model mice, reduce the mortality and delay dying time of mice in stimulative condition.

CONCLUSIONZhimu combined with Huangqi can slow down heart rate, enhance the reserve force of the heart, and improve the response capacity of cardiac hypertrophy mice in stimulative condition.

Anemarrhena ; chemistry ; Animals ; Astragalus membranaceus ; chemistry ; Cardiomegaly ; physiopathology ; Cardiotonic Agents ; pharmacology ; Cold Temperature ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Female ; Heart Rate ; drug effects ; Hot Temperature ; Isoproterenol ; antagonists & inhibitors ; Male ; Mice ; Myocardium ; pathology ; Physical Stimulation ; Plants, Medicinal ; chemistry

OBJECTIVETo observe the effect of Zhimu and Huangqi used singly or combinatively on improving experimental cardiac dysfunction, and mainly to observe zhimu's effect on restraining sympathetic nerve and blocking beta-adrenergic-recepter and huangqi's effect of improving hemodynamics on heart failure.

METHODTwo-clib one kidney operation was done to make renal hypertension rat model, 8 weeks after operation, rats were divided into groups and medicated for 6 weeks, and then their heart rate and blood pressure were measured, left ventricle was cannulated to estimate heart function, and heart-weight-index and left-ventricle-weight-index were measured.

RESULTZhimu could slow rats heart rate obviously, prevent cardiac remodeling, but did not affect cardiac function remarkably; Huangqi could reduce blood pressure, heighten +dp/dtmax and -dp/dtmax remarkably; the combined use of the two drugs could decrease plasma catecholamine concentration, adjust myocardium cAMP content, and improve heart function obviously.

CONCLUSIONZhimu and Huangqi can protect experimental cardiac dysfunction, and the combined use is better than the single use, which shows it better to use the two drugs combinatively in treating heart dysfunction.

Anemarrhena ; chemistry ; Animals ; Astragalus membranaceus ; chemistry ; Blood Pressure ; drug effects ; Cardiotonic Agents ; pharmacology ; Drug Synergism ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Heart Failure ; etiology ; physiopathology ; Heart Rate ; drug effects ; Hypertension, Renovascular ; complications ; physiopathology ; Male ; Plants, Medicinal ; chemistry ; Rats ; Rats, Wistar

Chinese Medical Association (CMA) is an academic society and the Chinese Medical Doctor Association (CMDA) is an administrative organization with academic character. The main functions of CMDA are setting up the system for specialty training, training for residents of different disciplines, establishing training bases, and safeguarding interests of doctors. The author also analyzes the drawbacks in this regard in field of burn surgery in China at the present time, and venture to put forward new suggestions for strengthening and perfecting the frame of CMDA.
Burns ; therapy ; Hospitals, Special ; manpower ; Humans ; Inservice Training ; Physicians ; Societies, Medical

Rehabilitation medicine is an emerging discipline. At present, there are many problems in rehabilitation of pediatric burn patients in China, including idea lag, weak technical force, lack of standardized technology, disunity in evaluation indexes, etc. The new era of reformation of health system and development and flourish of medical insurance business bring good opportunity for the development of rehabilitation medicine.
Burns ; rehabilitation ; Child ; China ; Humans

OBJECTIVETo investigate the influence of alpha smooth muscle actin fusion protein (alpha-SMA-FP) on fibroblast contraction, in order to find a new way to control scar contracture.

METHODSThree dimensional gel culture model of fibroblasts populated collagen lattices (FPCLs) was employed in the study. The fibroblasts were cultured in gel for 5 days. The cells in experimental group were processed by alpha-SMA-FP in dose of 5, 10, 50, 100 and 250 mg/L, respectively. The cells were therefore divided into E(1), E(2), E(3), E(4) and E(5) groups. Blank control was set to be C(1) group, and the cells processed by 250 mg/L of alpha-SMA-FP be C(2) group. The contraction rate was calculated by measuring the diameters of the gel before and after the procession. The change of contraction rate in E5 group was observed after the alpha-SMA-FP being rinsed out. Immunofluorescent staining of alpha-SMA-FP was carried out in fibroblasts.

RESULTSThe contraction rate in C1 and C2 groups showed no difference, being (58.6 +/- 3.1)% and (56.2 +/- 4.9)% respectively, while that in E1 to E5 groups was (45.56 +/- 4.1)%, (42.3 +/- 4.2)%, (41.8 +/- 3.6)%, (37.6 +/- 5.8)% and (26.4 +/- 4.7)%, respectively. However, the contraction rate in E5 was (53.3 +/- 5.6)% after the alpha-SMA-FP had been rinsed out. The difference of the rates among control group and experimental groups, especially in E5 after alpha-SMA-FP being rinsed out, was significant (P < 0.05 or 0.01). alpha-SMA-FP was located on the fibers of the fibroblasts as shown by staining, while the alpha-SMA was not stained. Nevertheless, the staining was obvious in control group.

CONCLUSIONalpha-SMA-FP could inhibit the contraction of fibroblasts specifically with dose dependent effect.

Actins ; administration & dosage ; pharmacology ; Animals ; Cells, Cultured ; Dose-Response Relationship, Drug ; Fibroblasts ; drug effects ; physiology ; Rats ; Rats, Wistar

Objective:To review the development and progress of geriatric research in China from the perspective of funding from the National Natural Science Foundation of China, and to provide supporting data for further development of the field.Methods:Based on project funding data of the National Natural Science Foundation of China for geriatric medicine from 2008 to 2017, the number and composition of projects, funding amount and funded institutions were statistically analyzed, and the overall trend of change was examined.The research hotspots in recent years were summarized by using word frequency analysis.Results:From 2008 to 2017, a total of 446 projects were approved in the field of geriatric medicine.Both the number of projects and the amount of funding showed an increasing trend.Project types were gradually expanded and the composition was constantly optimized.Since 2014, the fund types each year included 6 or more and breakthroughs in major projects were achieved.East China remained the leading region in the number of projects and amount of funding, with Beijing, Shanghai and Zhejiang receiving the highest numbers of project approvals and accounting for more than 10% of the national total in the number of project approvals and the amount of funding.During this period, 114 institutions received funding, with 74 winning 2 or more projects.In word frequency analysis, senescence(229 times), cell(161 times)and protein(120 times)were the three most frequently used keywords.Conclusions:In the field of geriatric medicine, the project approval level from the National Natural Science Foundation of China has significantly improved in quantity and quality, but it has always been under-represented in the branch of medical sciences.The regional and institutional distribution of projects is unbalanced and tends to skew in favor of institutions with prominent academic disciplines.Basic research is the main type of funded projects.Geriatric research is centered on the mechanisms of aging.

Objective To investigate the role of GABAAα3and GABAB receptors in the ventrolateral periaqueductal gray in the development of paw acute pain in rats.Methods Twelve male SD rats, weighing 280~320 g, were randomly divided into two groups: normal saline group (group NS), formaldehyde-induced pain group (group F), 6 rats in each group.In group F, rats were subcutaneously injected with 2% formaldehyde 50 μl into the ventral surface of right hind paw to induce periphery inflammatory pain.In group NS, rats were subcutaneously injected with normal saline into the ventral surface of right hind paw.Mechanical threshold was assessed using von Frey hairs for every ten minutes.The rat pain behavior scores were recorded for every five minutes.The thickness of skin and skin temperature were recorded for every fifteen minutes.Results Mechanical hyperalgesia were induced in group F after formalin injection into right hind paw.Compared with group NS, rat pain behavior scores were increased significantly in group F at all time points after injection, mechanical threshold were decreased significantly in group F at 10-60 min after injection, the temperature of the skin and the skin thickness were increased significantly in group F at 15-60 min after injection (P<0.05), the levels of the expression of GABAAα3 and GABAB were significantly increased in group F (P<0.05).Conclusion GABAAα3and GABAB receptors mediates formalin-induced hyperalgesia at ventrolateral portion of the PAG (vlPAG) of rats.