Objective:To investigate the feasibility of hemodialysis machine preventive maintenance model of based on quality control detection.Methods: Selected sample of 32 hemodialysis machines of two major imported brands, 16 of them using the traditional preventive maintenance mode for maintenance, and the other 16 sets of quality control detection based preventive maintenance hemodialysis machine maintenance mode. That: by use of HDM99XP detector instrument for testing the five hemodialysis machine main indicators, maintenance cycle is 16 weeks, and then the equipment failure rate indicators of two modes of preventive maintenance were compared.Results: The hemodialysis machine preventive maintenance model of based on quality control detection rise superior to the traditional maintenance mode in lower failure rate, quantitative, objective, and less affected by human factors.Conclusion: The hemodialysis machine preventive maintenance model of based on quality control detection was better than the traditional preventive maintenance mode,also a promising preventive maintenance mode.

Objective: To compare the differences in gene expression between murine intestine intraepithelial lymphocytes (iIELs) and splenic T lymphocytes, establishing the cDNA subtractive library of iIELs, analyzing the iIELs special genes. Methods: The iIELs and splenic T lymphocytes were isolated by density gradient in Percoll and nylon column respectively. cDNA subtractive library of iIELs was established by improved subtractive hybridization. The cDNA was ligated with a different adaptor. After 2 rounds of hybridization and suppression PCR amplification, the PCR products were directly inserted into the plasmid vectors. The ESTs special for iIELs were screened by reverse Northern blot. Results: Fifty ESTs with obvious difference in the cDNA subtractive library of iIELs were screened. These ESTs were squenced and submitted to Genebank. The ESTs were analyzed through BLAST, and 14 genes special for iIELs were obtained. These genes were associated with the pattern recognition of pathogen, cytokine signal transduction and proteinase inhibitors. Conclusion: iIELs are important components of intestine natural immunity, it can recognize the conservative antigens of bacteria and can be affected by cytokines and proteinase inhibitors, leading to unique cellular behavior--more resting and less activation.

Fracture Fixation ; methods ; Humans ; Mandibular Condyle ; injuries ; Mandibular Fractures ; complications ; therapy ; Maxillary Fractures ; complications ; therapy ; Multiple Trauma ; complications ; mortality

Abdominal Pain ; therapy ; Adult ; Aged ; Electroacupuncture ; Female ; Humans ; Male ; Middle Aged ; Rectal Diseases ; therapy ; Young Adult

Objective To explore the expression of circadian clock gene Per2 in the tissue of esophageal cancer and the relevance between VEGF,cyclin D1.Methods 80 cases with esophageal cancer who received surgical treatment and pathological specimens preserved were selected,and the normal tissues adjacent to carcinoma were used as control.The expression of Per2,VEGF and cyclin D1 were detected by immunohistochemistry,and the data were analyzed.Results Compared the tissue adjacent to carcinoma with carcinoma tissue,Per2,VEGF and cyclin D1 expression had statistically significant differences(x2 =26.86,24.34,23.72,all P ＜0.01).Per2 expression in different differentiation degree and the TMN stages had statistically significant differences (x2 =6.45,7.77,all P ＜ 0.05) ;cyclin D1 expression in lymph node metastasis and TMN stages had statistically significant differences(x2 =12.99,8.99,all P ＜ 0.01) ;VEGF expression in lymph node metastasis and TMN stages had statistically significant differences(x2 =6.68,6.14,all P ＜ 0.01).Per2 expression was negatively correlated with VEGF,cyclin D1 expression (r =-0.644,-0.523,all P ＜ 0.01).Conclusion Clock gene Per2 and VEGF,cyclin D1 expression in esophageal cancer has certain correlation,it can be a prognostic indicator of esophageal cancer.

Objective To understand the epidemiological characteristics of hypertension in Chifeng.Methods 6 015 subjects aged over 15 years old were selected with stratified randomized sampling.The history of hypertension and social-economic status were investigated by face-to-face interview and blood pressure,body height,body weight and waistline were measured at the same time.Results The prevalence of hypertension in Chifeng was 28.5%.The age-adjusted prevalence was 19.09%.Thereinto,the prevalence of hypertension in urban area,rural area,pasturing area were 23.15%,19.85%,32.03%,respectively.The Hypertension prevalence in male was increased with age.The prevalence in male was higher than in female among people who lived in urban and pasturing area(P0.05).The prevalence were higher in illiterate person and semiliterate than people in other educational level(P

Objective To optimized the culture conditions of the human umbilical cord blood mesenchymal stem cells.Methods The umbilical cord blood was taken in Guangzhou Hua Qiao Hospital between Oct.2004 to Feb.2005.The isolation method,planting density,the time of the first medium changing,the influence of culture medium on the growth of MSC were analyzed. Mesenchymal stem cells were identified using the surface marker by flow cytometry.Osteoblast was identified by Von Kossa staining and alkaline phosphatase staining,adipocyte was identified by Oil Red O staining. Results When the other conditions were the same,the hespan isolation was better than Ficoll isolation;5?10~6/cm~2 was the best planting density;the best first medium changing was on the seventh day at primary culture. Under optimized conditions,the MSC expressed adhesion molecules CD_ 13 ,CD_ 29 and CD_ 44 ,but not antigens of hematopoietic CD_ 34 ,CD_ 45 ,CD_ 14 and not antigens of endothelia CD_ 106 . Exposure of these cells to osteogenic inductive agents resulted in an increase in expression of alkaline phosphatase and the appearance of hydroxyapatite nodules. Incubation with adipogenic inductive agents resulted in morphological change and staining with Oil Red O. Conclusion Mesenchymal stem cells exist in Cord blood,but slower to establish in culture.Cord blood may prove to be a new source of cells for cellular therapeutics.

Adult ; Female ; Humans ; Male ; Middle Aged ; Palatine Tonsil ; pathology ; Sleep Apnea, Obstructive ; pathology ; surgery ; Tongue ; pathology

Objective To express functional haemegglutinin(HA)protein in two different bacularvirus expression systems.Methods The whole open reading frame of A/Sichuan/1/2009(H1N1)HA was obtained by synthesis,and the HA protein were expressed in insect cells by two different bacularvius expression systems:BaculoGold system and Bac-to-Bac system. Soluble HA protein was identified by Western blot and haemegglutination test. Results The correct full length of HA gene was obtained and cloned into pAcGP67B and pFAST Bacl vectors,respectively.After 3 rounds of virus amplifyjng by re-infection of Sf9 cells,the HA protein was detected in supematant of BaculoGold system and in intracellular of Bac-to-Bac system which is much better than the former.Purified HA protein was positive not only identified by Western blot,but also detected by haemegglutinin test. Conclusion Functional HA protein was successfully expressed in two distinct bacularvirus expression systems,of which the Bac-to-Bac bacularvirus expression system is more suitable for expression of A/Sichuan/1/2009(H1N1)HA protein.

OBJECTIVE: To investigate whether sodium magnesium fructose diphosphate(SM) could raise the contents of energy sustrates-ATP, ADP and AMP in the tissues of acute myocardial infarction.METHODS: 60 rat models of acute myocardial infarction were randomly divided into 6 groups(n = 10): Treatment groups(A, B, C) received SM(75mg/kg, 38mg/kg and 19mg/kg) in saline sulution respectively, and control groups (D, E) received 1,6 - fructose diphosphate (200mg/kg) or magnesium sulfate(10mg/kg), another control(F) received same volume of saline.Four hours after administration, the levels of ATP,ADP and AMP in the myocardial homogenate were determined by HPLC.RESULTS:The levels of ATP, ADP and AMP of treatment groups(A, B, C) were (612.8± 103.2)、 (538.0± 141.2) and (325.2± 113.2) nmol/g; (407.6± 113.4)、 (389.4±91.5)、 (306.1 ± 134.6) nmol/g and ( 1 637.6 ± 236.8)、 (1 366.8± 279.3)、 ( 1 186.1 ± 341.1) nmol/g; while those of control groups(F) were (119.0± 22.8)、 (146.5± 36.8)、 (436.7± 214.9) nmol/g, respectively(P＜ 0.01 of 0.05) .CONCLUSION: SM can raise the contents of energy substrates- ATP,ADP and AMP in the tissues of acute myocardial infarction.