Evidence-Based Medicine ; Humans ; Resuscitation ; Shock ; therapy

Animals ; Burns ; physiopathology ; therapy ; Genetic Engineering ; Humans ; Hyperbaric Oxygenation ; Tissue Engineering ; Wound Healing

Burns ; Evidence-Based Medicine ; Humans

Objective:To clone and express mouse fibroblast growth factor eight(FGF 8) and investigate the function of it Methods:According to the published sequence of mouse fibroblast growth factor 8, a pair of special primers was designed Then the full length cDNA of FGF 8b (a predominant isoform of FGF 8) was isolated from the total RNA of the mouse embryo by means of reverse transcription PCR and subcloned into the yeast expression vector of pYEX4T 1 in correct direction It was identified with sequencing The recombinant plasmid was transformed into yeasts The fusion protein expressed was verified by using Western blot The activity of the protein was examined by MTT and 3H TdR incorporation Results:The cDNA fragment was about 600 bps, and proved to be "b" isoform of FGF 8 exactly This fusion protein, with molecular weight about 55 kD, was highly expressed in yeast system The data of MTT and 3H TdR incorporation in NIH3T3 cells were increased obviously by the protein, and decreased by the antagonist Conclusion:Fibroblast growth factor eight was cloned and expressed.The protein was found to be capable of stimulating the proliferation of NIH3T3 cells Moreover, the recombinant protein of the extracellular fragment of FGFR1 can antagonize the response of NIH3T3 to the recombinant protein

Objective:To investigate the feasibility of hemodialysis machine preventive maintenance model of based on quality control detection.Methods: Selected sample of 32 hemodialysis machines of two major imported brands, 16 of them using the traditional preventive maintenance mode for maintenance, and the other 16 sets of quality control detection based preventive maintenance hemodialysis machine maintenance mode. That: by use of HDM99XP detector instrument for testing the five hemodialysis machine main indicators, maintenance cycle is 16 weeks, and then the equipment failure rate indicators of two modes of preventive maintenance were compared.Results: The hemodialysis machine preventive maintenance model of based on quality control detection rise superior to the traditional maintenance mode in lower failure rate, quantitative, objective, and less affected by human factors.Conclusion: The hemodialysis machine preventive maintenance model of based on quality control detection was better than the traditional preventive maintenance mode,also a promising preventive maintenance mode.

Objective: To explore the expression of MMP-9 in mouse brain tissue and its significance. Methods: Cryptococcosis ssuspension alone (fungal suspension group) or combined with aprotinin (aprotinin group) was injected in to female C57 BL/6 mice via femoral vein cannulation. The expression of MMP-9 was examined immunohistochemically 8 h later. Meanwhile, the mouse brain tissue homogenate colony count (CFU) was observed and the results were compared with that of the immunohistochemical examination. Results: The expression of MMP-9 was markedly increased in the fungal suspension group compared with the normal control group (P<0.01); the expression in the aprotinin group was not significantly different from that of the normal control group (P>0.05). The amount of cryptococcosis in the brain tissue was positively correlated with expression of MMP-9. Conclusion: MMP-9 is overexpressed in the brain of mice with Cryptococcal meningitis; MMP-9 might lead to increased permeability of blood brain barrier.

Adolescent ; Adult ; Female ; Humans ; Male ; Niemann-Pick Diseases ; genetics ; Pedigree

We report an autopsy case of congenital cytomegalic inclusion disease that was associated with massive periventricular calcification and hydrocephalus. The male boy was bron by cesarian section at 38 weeks of gestational age to a 28 year old mother and was dead two hours after delivery because of respiratory difficulty. Radiologic findings showed characteristic linear periventricular calcification, being associated with marked dilatation of the lateral ventricles. At autopsy, the brain parenchyme became 0.4 cm in average thickness due to obstructive hydrocephalus at aqueductal level. The inner surface of the lateral ventricle showed grayish white granular appearance that could correspond to the calcification seen on plain skull X-ray films. Although active ependymitis, destruction of the brain parenchyma with dystrophic calcification and gliosis were noted, there was no intranuclear inclusion. After scrutinizing many sections of various organs and tissue, we could be able to demonstrate a few inclusion bodies, highly suggestive of cytomegalic inclusion disease. It was discussed that this case might suggest that intranuclear inclusions in cytomegalovirus infection could by very difficult to find particularly when the infection is in inactive phase despite the presence of tissue necrosis and calcification of the brain.
Male ; Humans

Objective To study on comparison of different age group patients with acute abdomen who received laboratory check-ups.Methods 126 patients with acute abdomen were divided into 0.05).2.Of 126 patients,65 cases(51.6%)received abdominal B ultrasonography check-up,among which 41 cases(44.1%)were in

Objective: To evaluate the activities of extracellular proteinase and serine protease secreted by Cryptococcus neoformans (C. neoformans) of different sources and serotypes and incubated under different conditions. Methods: Thirty-six C. neoformans strains of different sources and serotypes were incubated in protein agar medium at 30°C and 37°C separately. Activities of extracellular proteinase and serine protease were analyzed by determining Clearance Halos(CHs) produced by C. neoformans. Activity changes of the 2 proteases were analyzed after treated with serine protease inhibitors. The activity and the molecular weight of extracellular proteinase in the concentrated supernatant were measured using zymography assay. Results: Thirty-three of 36 C. neoformans strains produced specific Clearance Halos around the colonies, and their mean CHs at 30°C and 37°C were 0.558±0.170 and 0.575±0.169, respectively (P>0.05). The mean CHs of serotype A(n=13), B(n=13), and D/AD (n=6) strains were 0.564±0.144, 0.515±0.078 and 0.482±0.072, respectively (P>0.05); the mean CHs of clinical(n=23), environmental(n=9),and uncapsuled strains(n=4) were 0.570±0.177, 0.513±0.069, and 0.942±0.075, respectively (P<0.05). The mean CHs of control group (without protease inhibitor) and groups treated with protease inhibitor (1.2 mU and 1.6 mU) were 0.459±0.188, 0.975±0.287 and 0.733±0.252, respectively(P<0.01). Rich extracellular protease was found in the concentrated supernatant. Conclusion: The incubation temperature (30°C and 37°C) has no effect on activities of extracellular proteinase and serine protease, and the activities of clinical strains are significantly stronger than those of other 2 strains; the activities in different serotypes groups have no significant difference and can be inhibited by serine protease inhibitors.