Facial Nerve ; Facial Nerve Diseases ; Facial Nerve Injuries ; Facial Paralysis ; Neurology ; trends

China ; Cochlear Implantation ; trends ; Cochlear Implants

BACKGROUND: The endothelial-mesenchymal transition is known to play a central role in the pathological process of heterotopic ossification. OBJECTIVE: To verify the inhibitory effect of SMAD7 on endothelial-mesenchymal transition such as myofibroblast transformation and to explore whether it is a potential target for heterotopic ossification.METHODS: A lentivirus overexpressing SMAD7 was contructed and the optimal titre and transfection efficiency in rat aortic endothelial cells were determined. The lentivirus was then injected into a rat model of Achilles tendon injury, while the controls were given the injection of normal saline. Expressions of endothelial and mesenchymal markers at the injured site were analyzed by quantitative PCR and western blot assay. The heterotopic ossification was observed radiologically and histologically.RESULTS AND CONCLUSION: Local injection of SMAD7-delivering lentivirus resulted in an upregulation of CD31 and VE-cadherin, and a downregulation of N-cadherin and vimentin, suggesting that the endothelial-mesenchymal transition is blocked due to local SMAD7 overexpression. The inhibitory effect became more evident at 10 weeks than at 6 weeks after modeling. Radiology and histological staining further confirmed that the ossified structures in the tendon tissue disappeared after injection of SMAD7-delivering lentivirus, as opposed to the control group. These data suggest that local overexpression of SMAD7 can prevent postoperative heterotopic ossification with no effect on wound healing.

Objective To explore the relationship of T2DM with xerophthalmia after cataract surgery.Methods Retrospective analysis was made on the clinical data of 137 cataract patients (176 eyes) from April,2016 to October,2016.The DM patients were chosen as the DM group (58 cases,71 eyes),and single cataract ones were chosen as the control group (79 cases,105 eyes).The clinical data and xerophthalmia index were compared between two groups.Results The age and sex constituent ratio of DM group and control group had no significant difference (all P ＞ 0.05).The postoperative MQ score,BUT,FL,SⅠt of DM group at 1 week,1 month and 3 months were significantly worse than those at preoperative 2 days (all P ＜ 0.01),but the indexes relieved with time prolong.The postoperative MQ score,FL,SⅠt of control group at 1 week,1 month were significantly worse than those at preoperative 2 days (P ＜ 0.01),the postoperative BUT and preoperative BUT had no significant difference (P ＞ 0.05),all of the postoperative index and preoperative index had no significant difference(all P ＞ 0.05).The postoperative MQ score,BUT,FL,SⅠt of DM group at 1 week,1 month and 3 months were significantly worse than those of the control group (all P ＜ 0.01).Conclusion The xerophthalmia after cataract surgery of T2DM patients are more seriously than normal cataract patients.

Adult ; Ear Canal ; Ear Neoplasms ; Humans ; Male ; Sarcoma, Myeloid

Cholesteatoma ; classification ; Humans ; Microsurgery ; Otitis Media ; classification

Humans ; Vertigo ; diagnosis ; physiopathology ; Vestibular Function Tests

[Objective]To study the tendency of hip fracture by analyzing morbidity factors to provide an basis for preventing the fracture.[Method]All of the case records of hip fractures treated at west china hospital between 2000-1 and 2005-12 were checked and 583 inpatients were selected for analysis.[Result]The major injury cause was fall in the old and female was more susceptible.High energy injury was the major cause in younger 50. Osteoporosis and fall contributed best to the hip fracture in elderly.Concomitant disease and environmental factors increased the risk.[Conclusion]Osteoporosis and fall contribute most to the hip fracture in elderly,accordingly,to prevent fall and osteoporosis is vital to the old.

Objective To explore the change of liver mitochondrial calcium metabolism and its effect on mitochondrial respiratory function.Methods The model of dog biliary obstruction was made by bile duct ligation. Mitochondria were obtained by differential centfifugation.Mitochondrial calcium content,calcium uptake rate and respiratory function were observed and compared.Results Liver mitochondrial calcium content was increased sig- nificantly,calcium uptake rate and respiratory function were obviously reduced after biliary obstruction.Mitochon- drial calcium content was highly negatively correlated with mitochondrial respiratory function (P