1.A new culture system for in situ observation of the growth and development of Eucyclops serrulatus (Copepoda: Cyclopoida) .
Sung Hee PARK ; Cheon Young CHANG ; Sung Shik SHIN
The Korean Journal of Parasitology 2005;43(4):141-147
A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a microscope slide glass on which a cover glass of 22 x 40 mm in size was mounted at a height of 0.8 mm. The culture medium was constituted by bottled mineral water boiled with grains of Glycine max (soybean). Chilomonas paramecium, a free-living protozoan organism, was provided as live food. Growth of nauplii hatched from eggs to the first stage of copepodite took an average of 7.7 days, and the growth of copepodite 1 to the egg-bearing adult female took an average of 20.1 days in the microculture cell with an average life time of 44.7 days. Continuous passage of copepods was successfully maintained as long as sufficient medium and food were provided. The microculture method enables an in situ microscopic observation on the growth and developmental process of helminth larvae experimentally infected to copepods as well as of copepod itself. Furthermore, it does not require anesthetization and, therefore, minimize the amount of stress exposed to copepods during the handling process.
Protozoa
;
Male
;
Female
;
Culture Techniques/*methods
;
Culture Media
;
Copepoda/*growth & development
;
Animals
2.Pathogenesis and Surgical Treatment of Rectal Prolapse Syndrome.
Jin Cheon KIM ; Chang Nam KIM ; Sang Kyu PARK ; Sook Young KIM ; Chang Sik YU
Journal of the Korean Society of Coloproctology 1998;14(2):225-234
The rectal prolapse syndome is a disease entity includes rectocele and rectal prolapse, presenting prolapse(procidentia) of rectum. In rectocele, rectum is prolapsed anteriorly into the vagina, whereas in procidentia, inferiorly out of the anus. This study was aimed at analyzing pathogenesis and adequacy of surgical treatment in rectocele and rectal prolapse. Twenty-one patients with rectocele and 18 patients with rectal prolapse were assessed pre- and post-operatively in respect to symptoms and signs, pathogenesis, defecography, and manometry. In analysis of symptoms and sings, constipation was the commonest in both diseases(86% of rectocele and 67% of rectal prolapse) and incontinence was not infrequently found in both diseases as well(14% of rectocele and 33% of rectal prolapse). In analysis of the underlying causes, two patients with rectal prolapse had prolapse from childhood. Defecography showed anorectal angle of rectal prolapse in rest and push period. They were significantly wider than those of rectocele(p<0.05). The perineal descent of rectal prolapse was longer than that of rectocele. In analysis of the associated factors, average number of delivery was more than three times in both diseases(3.5 of rectocele and 5.1 of rectal prolapse). We could easily find previous operation history in both diseases. Among them, hysterectomy was the most frequent, especially in patients with rectocele. The hemorrhoids was associated more common in rectocele than in rectal prolapse(p<0.05). Preoperative maximal resting pressure of rectal prolapse was more significantly decreased than that of rectocele(p<0.05). The sensation of fullness was significantly decreased in patients with rectal prolapse postoperatively(p<0.05). Patients with rectocele underwent levator plication by transrectal or vaginal approach. Patients with rectal prolapse underwent posterior rectopexy in 11 patients, resection and rectopexy in 3 patients, Delorme's operation and Thiersch operation in 2 patients each. Constipation was significantly improved in patients with rectocele postoperatively(p<0.05). Incontinence was markedly improved in patients with rectal prolapse postoperatively(p<0.05). At the interview about subjective improvement of symptom, 95% of patients with rectocele and 89% of patients with rectal prolapse were satisfied with surgery. In conclusion, rectocele and rectal prolapse can be categorized as rectal prolapse syndrome because both diseases have anatomical derangements caused by similar pathogenesis such as altered bowel habits, anatomical factor, delivery, past history of hysterectomy, and hemorrhoids. Levator plication and posterior rectopexy seem to be useful surgical methods of anatomical repair for the respective disease.
Anal Canal
;
Constipation
;
Defecography
;
Hemorrhoids
;
Humans
;
Hysterectomy
;
Manometry
;
Prolapse
;
Rectal Prolapse*
;
Rectocele
;
Rectum
;
Sensation
;
Vagina
3.A Case of Patient with Common Variable Immunodeficiency.
Keun Cheon KIM ; Chang Gee KANG ; Chang Hyun YANG ; Dong Soo KIM ; Kir Young KIM
Journal of the Korean Pediatric Society 1989;32(8):1123-1127
No abstract available.
Common Variable Immunodeficiency*
;
Humans
4.Prognostic Significance of the Expression of MRP and p53 in Colorectal Carcinoma.
Ji Young CHANG ; Young Don MIN ; Kweon Cheon KIM ; Sung Chul LIM
Journal of the Korean Society of Coloproctology 1998;14(3):365-374
Although MDR was previously thought to be predominantly caused by the expression of the MDR1 gene, it is now increasingly believed to be caused by other mechanism. Recently, over-expression of the multidrug resistance-associated protein (MRP) was suggested a possible mechanism for non-Pgp mediated MDR. Recent studies showed that MRP can confer resistance to a wide spectrum of natural product drug, but the clinical relevance of MRP-mediated MDR in human cancer is poorly understood. p53 is the most widely known tumor suppressor gene. It has been suggested that mutant p53 is related to abnormal proliferation of cell and some what is been related to cellular apoptosis. To determine the clinical significance of MRP and/or p53 expression in colorectal carcinoma, the authors investigated the expression of the MRP and p53 in 81 cases of primary colorectal carcinoma, the relationship between the MRP and/or p53 expression and clinical parameters including 5-yr. survival rate, and the relationship between the expression of MRP and p53. The results were as follows: 1) Of the 81 colorectal carcinomas, 36 (42%) were MRP positive and 28 (34%) were p53 positive. 2) The expression of MRP and/or p53 was not significantly correlated with sex, age, histologic grades, tumor invasion, tumor location, tumor size, lymph node metastasis, TNM stage and survival of patients. In conclusion, these results suggest that expression of MRP and/or p53 is neither related to the known prognostic factors nor a prognostic factor by itself.
Apoptosis
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Colorectal Neoplasms*
;
Genes, Tumor Suppressor
;
Humans
;
Lymph Nodes
;
Multidrug Resistance-Associated Proteins
;
Neoplasm Metastasis
;
Prognosis
;
Survival Rate
5.Pattern of Adenomas after Colorectal Cancer Surgery.
Shin HWANG ; Won Young HWANG ; Chang Sik YOO ; Hee Won CHUNG ; Jin Cheon KIM
Korean Journal of Gastrointestinal Endoscopy 1996;16(2):191-198
Postoperative colonoscopy is an effective tool for management of colonic adenomas. Authors analyzed the pattern of colonic adenomas detected during follow-up colonoscopy after colorectal cancer surgery and evaluated the characteristics of adenornas, risk groups, and effective fo1low-up schedule. Study group were 222 patients and colonoscopy was performed 389 times. Patterns of adenornas were analyzed by variables as age, sex, preoperative serum CEA level, location of primary colorectal cancer, Borrmann type, Duke's stage, histologic differentiation, DNA ploidy, recurrence and histology. Metachronous adenomas were detected in 79 patients(35.6%) and both metachronous and syachronous adenomas were observed in 29 cases(13.1%). High risk variables for adenomas were male, old age and presence of synchronous adenoma. There were 2 patients with maligant change of adenomas. Yearly follow-up by complete colonoscopy over 3 years or more is recommended and follow-up interval should be shortened in the high risk groups.
Adenoma*
;
Appointments and Schedules
;
Colon
;
Colonic Neoplasms
;
Colonoscopy
;
Colorectal Neoplasms*
;
DNA
;
Follow-Up Studies
;
Humans
;
Male
;
Ploidies
;
Recurrence
6.A case of primary malignant melanoma of hard palate mucosa.
Yoon Young CHUNG ; Seung Ju LEE ; Cheon Hwan OH ; Chang Jin KIM
Korean Journal of Otolaryngology - Head and Neck Surgery 1992;35(4):600-604
No abstract available.
Melanoma*
;
Mucous Membrane*
;
Palate, Hard*
7.Comparison of measurements of airway resistance during panting and quiet breathing.
Seon Hee CHEON ; Woo Hyung LEE ; Kee Young LEE ; Se Kyu KIM ; Joon CHANG ; Sung Kyu KIM ; Won Young LEE
Tuberculosis and Respiratory Diseases 1993;40(3):267-273
No abstract available.
Airway Resistance*
;
Respiration*
8.Gluteus Maximus Transposition for Anal Incontinence.
Ji Young CHANG ; Cheong Tong KIM ; Kweon Cheon KIM ; Young Don MIN
Journal of the Korean Society of Coloproctology 1997;13(1):137-144
Anal incontinence following pelvic trauma, surgery or neurologic disorder has significant medical and social implications. When no known functioning sphincter muscles are present, surgical correction of this distressing condition other than by stomal fecal diversion is aimed at recreating a sphincter mechanism under voluntary control. The use of the gluteus maximus encircling the neorectum with a contractile muscualr ring provides an active control of continence and reserves the anorectal angulation. The sacrifice of the entire gluteus maximum muscle in an ambulatory patient will cause difficulty in climbing stairs; however, the use of the anatomically dissected lower half will preserve its function. With careful dissection, the lower half of the g1uteus maximus muscle together with its neurovascular supply can be developed for anal sphincter reconstruction. Three Patient, (two men and one woman) underwent g1uteus maximus transposition for complete anal incontinence. The indication for operation were sphincter destruction secondary to extensive soft tissue necrosis on perianal, perineal and buttock area due to necrotizing fascitis(n=2), and soft tissue defect on perianal, buttock area due to trauma(n=1). The procedure is performed with the use of a diverting colostomy. The inferior portion of the origin of each gluteus maximus is detached from the sacrum and coccyx, bifurcated,and tunneled subcutaneously to encircle the anus. The ends were sutured together to form two opposing slings of voluntary muscles. Postoperatively two patient regained continence to solid stool, one to liquid stool as well. The technique of constructing sphincter is simple and utilizes principles of muscle tendon transfer without jeopardizing function of gait. Furthermore the gluteus maximus muscle, being an accessory muscle of anal continence, is an ideal structure for this reconstruction.
Anal Canal
;
Buttocks
;
Coccyx
;
Colostomy
;
Fecal Incontinence
;
Gait
;
Humans
;
Male
;
Muscle, Skeletal
;
Muscles
;
Necrosis
;
Nervous System Diseases
;
Sacrum
;
Tendon Transfer
9.An Experimental Study of Silastic Cuff Wrapping Around the Severed Peripheral Nerve
Hong Chul LIM ; Woo Nam MOON ; Jae Young CHEON ; Jay Suck CHANG ; Seok Hyun LEE ; In Young SUNG
The Journal of the Korean Orthopaedic Association 1987;22(1):14-22
The authors conducted an in-Vivo experimental study using sciatic nerve of 200-300 grammed rats to verify clinical applicability of Silicone cuffing method, by which scar adhesion of repaired nerve in crushed limb is assumed hopefully to be reduced. The following observations were obtainable. 1. Nerve conduction velocity was noted faster in Silicone cuff group than in control group significantly after 6 weeks postoperatively. 2. Adhesion of repaired nerve with surrounding scar tissue was far less present in Silicone cuff group than in control group when observed grossly. 3. The amount of scar formation and axonal degeneration were noted reduced remarkably in Silicone cuff group than in control group after 3 weeks postoperatively. 4. The intraneural vascularity was noted reduced either in Silicone cuff group and in control group, but definitely less reduced in the former than in the latter. Clinical relevance: Above results may be taken as an experimental basis supporting clinical applicability of Silicone cuff wrapping method for neurorraphy in crushed limb.
Animals
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Axons
;
Cicatrix
;
Extremities
;
Methods
;
Neural Conduction
;
Peripheral Nerves
;
Rats
;
Sciatic Nerve
;
Silicon
;
Silicones
10.Assessment of the relationship between pulmonary function test and dyspnea index in patients with bronchial asthma.
Se Kyu KIM ; Seon Hee CHEON ; Joon Ha CHANG ; Won Hong JONG ; Soo CHEIN ; Sung Kyu KIM ; Won Young LEE
Tuberculosis and Respiratory Diseases 1992;39(5):392-399
No abstract available.
Asthma*
;
Dyspnea*
;
Humans
;
Respiratory Function Tests*