1.Expression and use of Truncated Recombimnant Flagellin Protein ( FlaB ) in ELISA for Diagnosis of Leptospirosis.
Cheon Kwon YOO ; Myo Ah PAIK ; Hae Kyung LEE ; Man Suck PARK ; Mi Yeoun PARK
Journal of the Korean Society for Microbiology 2000;35(5):365-365
No Abstract Available.
Diagnosis*
;
Enzyme-Linked Immunosorbent Assay*
;
Flagellin*
;
Leptospirosis*
2.Infection of Extended-Spectrum β-Lactamase Producing Shigella flexneri in Children Attending a Childcare Center in Korea.
Eun Woo NAM ; Kun Song LEE ; Junyoung KIM ; Cheon Kwon YOO
Pediatric Infection & Vaccine 2016;23(3):223-228
Shigella is a common cause of bacterial enteritis worldwide. Shigella sonnei accounts for 90% of Shigella infections and Shigella flexneri is rarely reported in Korea. Although the incidence of Shigella infection has decreased, the incidence of organisms with antibiotic resistance has gradually increased in Korea. An outbreak of extended-spectrum β-lactamase (ESBL)-producing S. sonnei in children was reported in Korea; however, ESBL-producing S. flexneri has not yet been reported. We report the first two cases of multidrug-resistant CTX-M-14-producing S. flexneri infections in Korean children.
beta-Lactamases
;
Child*
;
Drug Resistance, Microbial
;
Enteritis
;
Humans
;
Incidence
;
Korea*
;
Shigella flexneri*
;
Shigella sonnei
;
Shigella*
3.The First Outbreak of Botulism in Korea.
Gyung Tae CHUNG ; Do Hyun KANG ; Cheon Kwon YOO ; Jong Hyun CHOI ; Won Keun SEONG
Korean Journal of Clinical Microbiology 2003;6(2):160-163
Botulism is a rare neuroparalytic disease caused by neurotoxins of Clostridium species. A ten-year-old girl and her mother were admitted to a hospital with symptoms of progressive dizziness, blurred vision, slurred speech, constipation and difficulty in swallowing. These characteristic manifestations and clinical course prompted an examination of the possibility of botulism. Mouse bioassay performed with mother's stool demonstrated type A botulinum toxin and culture of the mother's stool was positive for Clostridium botulinum type A. This is the first case of botulism in Korea.
Animals
;
Biological Assay
;
Botulinum Toxins
;
Botulism*
;
Clostridium
;
Clostridium botulinum
;
Clostridium botulinum type A
;
Constipation
;
Deglutition
;
Dizziness
;
Female
;
Humans
;
Korea*
;
Mice
;
Mothers
;
Neurotoxins
4.Treatment of Transverse Patellar Fracture with Cannulated Screws.
Jung Man KIM ; Ju Seok YOO ; Yong Jin KWON ; Jang Ok CHEON
Journal of the Korean Fracture Society 2007;20(2):149-153
PURPOSE: To assess the indication and effect of screw fixation in the transverse patellar fractures. MATERIALS AND METHODS: We analysed the results of 14 transverse patellar fractures fixed with screws from January 1991 to May 2005. Mean follow-up period was 47 months (range, 12~143 months). We analysed the radiologic union, operation time, ROM and postoperative Lysholm score. RESULTS: All fractures healed uneventfully. The mean displacement was decreased from 2.2 mm preoperatively to 0.3 mm postoperatively (p=0.001, Wilcoxon signed rank test). The mean operation time was 34 minutes (range, 20 to 60 minutes). Normal range of motion was achieved in 13 knees (92.9%). Average Lysholm score was 95.9 at final follow-up. CONCLUSION: Screw fixation seemed to be useful for treatment of transverse patellar fracture even in comminuted fractures with large fragments. The advantage of this technique was the preservation of extensor mechanism, simplicity, short operation time and good cosmesis.
Follow-Up Studies
;
Fractures, Comminuted
;
Knee
;
Patella
;
Reference Values
5.Changes of Antioxidant Capacity in Colorectal Cancer Patients.
Deuk Young KWON ; Han Il LEE ; Ki Hyuk PARK ; Dae Hyun JOO ; Sung Whan PARK ; Yong Oon YOO ; Ki Ho PARK ; Chang Ho JEON ; Jong Yul CHEON ; Jin CHEON
Journal of the Korean Society of Coloproctology 1999;15(3):151-158
PURPOSE: Free radicals are defined as any species capable of independent existence that contains one or more unpaired electrons and they have the effects on carcinogenesis and tumor progression by causing mutations on genetic structure or suppressing repair of mutated DNA. This study was aimed to identify changes of antioxidant capacity in carcinogenesis and tumor progression of colorectal cancer (CRC). METHODS: We studied the level of serum antioxidant capacity using ABTS technique for 34 CRC patients who were operated between July 1997 and January 1998 at department of surgery, Taegu Catholic Medical Center and compared with 38 persons who had normal value of liver function during the same period. RESULTS: CRC patients showed decreased serum antioxidant capacity level compared to that of control group (CRC male 1.23 0.15 mmol/L, CRC female 1.11 0.13 mmol/L, control male 1.40 0.15, control female 1.35 0.11 mmol/L). Changes of antioxidant capacity levels were not correlated with stages, even though the marginal difference between T-stages (T1/2 1.23 0.10 mmol/L, T3/4 1.16 0.15 mmol/L). CONCLUSIONS: Free radicals may be the causative agent of colorectal carcinogenesis and can be associated with early phase of carcinogenesis rather than tumor progression.
Carcinogenesis
;
Colorectal Neoplasms*
;
Daegu
;
DNA
;
Female
;
Free Radicals
;
Genetic Structures
;
Humans
;
Liver
;
Male
;
Reference Values
6.Modified Method of Multilocus Sequence Typing (MLST) for Serotyping in Salmonella Species.
Young Sun YUN ; Su Jin CHAE ; Hye Young NA ; Gyung Tae CHUNG ; Cheon Kwon YOO ; Deog Yong LEE
Journal of Bacteriology and Virology 2015;45(4):314-318
Multilocus sequence typing (MLST) of Salmonella is useful method for replacing serotyping using antisera but is limited by difficulties associated with in polymerase chain reaction (PCR). We optimized the PCR reaction, especially annealing temperature and extension time (94degrees C for 2 min; 40 cycles at 94degrees C for 30 sec, 56.8degrees C for 1 min, 72degrees C for 2 min; and 72degrees C for 10 min). The degradation of PCR product by thermostable nucleases was inhibited by using template DNAs treated proteinase K or purified by a commercialized preparation kit. The resulting modified MLST was used as accurate and fast typing method.
DNA
;
Endopeptidase K
;
Immune Sera
;
Multilocus Sequence Typing*
;
Polymerase Chain Reaction
;
Salmonella*
;
Serotyping*
7.Bilateral Cortical Blindness Due to Parieto-Occipital Infarction after General Anesthesia: A case report.
Ki Ryang AHN ; Jong Kuk CHOI ; Chun Suk KIM ; Kyu Sik KANG ; Ji Eun KIM ; Si Hyun YOO ; Jin Hyung KWON
Korean Journal of Anesthesiology 2004;47(1):122-125
Cortical blindness is characterized by visual sensation loss with retention of pupillary reaction to light, and a normal fundoscopic examination. The suggested causes are emboli, profound hypotension, anemia, and infarction of watershed areas in the parietal or occipital lobe. We experienced a case of cortical blindness with severely reduced visual acuity after penile cancer surgery under general anesthesia. In the acute stage, visual acuity was slightly improved, but over the course of several months, no further improvement in visual acuity occurred. In this case there was no severe hypotension or anemia during the operation. Two days after the operation, electroencephalography (EEG) was performed during the period of blindness and the recording obtained was abnormal, with no alpha rhythm. Biparieto-occipital lucency was found by magnetic resonance imaging (MRI). Therefore parieto-occipital infarction due to seizure, embolus, or thrombosis could be considered a possible etiology. We concluded that cortical blindness can unexpectedly develop perioperatively and postoperatively, and that close monitoring of the patient and adequate management are essential.
Alpha Rhythm
;
Anemia
;
Anesthesia, General*
;
Blindness
;
Blindness, Cortical*
;
Electroencephalography
;
Embolism
;
Humans
;
Hypotension
;
Infarction*
;
Magnetic Resonance Imaging
;
Male
;
Occipital Lobe
;
Penile Neoplasms
;
Rabeprazole
;
Seizures
;
Sensation
;
Thrombosis
;
Visual Acuity
8.The Laboratory Diagnosis of Melioidosis in a Korean Patient.
Yong Woo SHIN ; Min Hee CHO ; Jeong Hoon CHUN ; Changmu KIM ; Hee Bok OH ; Gi eun RHIE ; Cheon Kwon YOO
Journal of Bacteriology and Virology 2011;41(1):19-25
Burkholderia pseudomallei is a gram-negative opportunistic intracellular pathogen that causes an acute and fatal septicemic melioidosis in humans. The organism is mainly found in Southeastern Asia and Northern Australia. Recently, we encountered a case of melioidosis in a Korean patient and performed the laboratory diagnosis of melioidosis. As a result, a gram negative bacterium was isolated from a melioidosis patient, and it was identified as B. pseudomallei on DNA sequencing of 16S ribosomal RNA with 99.9% homology and biochemical examination of VITEK gram-negative identification card. Also, DNA from cultured bacteria was tested in multiplex PCR, a 245 bp fragment amplified from the metalloprotease gene and a fragment of variable size ranging from 400~700 bp resulting from amplification of the 10 bp repetitive element for B. pseudomallei were confirmed after electrophoresis. The bacterium was sensitive to ceftazidime, imipenem and meropenem but resistant to ticarcillin. So far, there are no domestic cases of melioidosis in Korea, however, due to the increase in international travelers, the incidence of melioidosis is likely to increase. We report a recent case of melioidosis in a Korean patient.
Asia, Southeastern
;
Australia
;
Bacteria
;
Burkholderia pseudomallei
;
Ceftazidime
;
Clinical Laboratory Techniques
;
DNA
;
Electrophoresis
;
Humans
;
Imipenem
;
Incidence
;
Korea
;
Melioidosis
;
Multiplex Polymerase Chain Reaction
;
RNA, Ribosomal, 16S
;
Sequence Analysis, DNA
;
Thienamycins
;
Ticarcillin
9.Multiple Symmetric Lipomatosis: Characteristics and Treatment in Korean Patients.
Young Woo CHEON ; Tae Suk ROH ; Yong Oock KIM ; Ji Eun KWON ; Kwan Chul TARK ; Won Min YOO
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2007;34(4):477-483
PURPOSE: Multiple symmetric lipomatosis (MSL) is a relatively rare disorder characterized by presence of multiple, symmetric, nonencapsulated fat masses in face, neck, shoulder and other areas. There has been only a few cases reported in Korea. The main purpose of this research is to examine the Korean patients to see what kinds of special characteristics occurred due to this disease and to decide the proper treatment. METHODS: A total of 16 patients were evaluated retrospectively. 5 patients were treated at our hospital. The other patients were reviewed from literature. We analyzed the biological characters of patients, location of fat deposit, morphologic characters of patients, clinical evidence of neuropathy, associated metabolic disorders and treatment modality. RESULTS: All cases were male patient. The mean age of onset was 47.43 years. All patients were moderate to heavy alcoholics. The most common location of fat deposition was posterior neck and abdomen. In neurologic exam of 9 patients, 5 patients showed muscle weakness, tremor, pain and autonomic nerve dysfunction. In metabolic studies of 9 patients, total cholesterol values were higher in 1 patient. A glucose tolerance test was abnormal in 1 patient. In treatment modality, 14 patients were treated with surgical resection, 1 patient was treated with liposuction and surgical excision, 1 patient was treated only with liposuction. CONCLUSION: To treat MSL patients successfully, we should concentrate not only on the removal of the fatty tissue but also on neurologic abnormities, metabolic disorders and associated diseases.
Abdomen
;
Adipose Tissue
;
Age of Onset
;
Alcoholics
;
Autonomic Pathways
;
Cholesterol
;
Glucose Tolerance Test
;
Humans
;
Korea
;
Lipectomy
;
Lipomatosis, Multiple Symmetrical*
;
Male
;
Muscle Weakness
;
Neck
;
Retrospective Studies
;
Shoulder
;
Tremor
10.Genetic Diversity among Virulent Mega Plasmids pXO1 and pXO2 of Bacillus anthracis Isolated in Korea.
Sung Hee MUN ; Cheon Kwon YOO ; Hee Bok OH ; Won Keun SEONG ; Jeong Hoon CHUN ; Jae Yon YU ; Sang Seob LEE
Journal of Bacteriology and Virology 2003;33(4):253-264
We compared genetic variations in virulence mega plasmids pXO1 and pXO2 of twenty-seven Bacillus anthracis strains from Korean patients and environmental samples together with those of Bacillus anthracis Sterne, Pasteur and A2012 standard strains. Genetic variations were analyzed in twenty-three variable regions (ten and thirteen variablenumber tandem repeats and insertion/deletions in pXO1 and pXO2, respectively). The pXO1 plasmids were classified into 7 groups and pXO2 plasmids to 12 groups. Discrete phylogenic lineages could be differentiated between environmental and clinical strains by UPGMA (unweighted pair group method with average) method. In addition, clinical strains showed more variations than environmental isolates. The pXO2 plasmid appeared genetically more unstable than pXO1. A general plasmid genotype could be suggested for Korean soil isolates since they mostly clustered into a representative group.
Bacillus anthracis*
;
Bacillus*
;
Genetic Variation*
;
Genotype
;
Humans
;
Korea*
;
Plasmids*
;
Soil
;
Tandem Repeat Sequences
;
Virulence