No abstract available.

One hundred seven isolates of Staphylococcus aureus from bovine mastitis were investigated for colony morphology in serum-soft agar (SSA), autoagglutination in salt, and capsular serotype. Capsular polysaccharide (CP) was purified and quantified from the extracts of clinical isolates. Overall, 89 isolates (83.2%) were diffuse in the SSA, without any difference in the proportion of diffuse colony between type 5 and type 8 strains. Some strains exhibited compact colonies in the SSA and expressed CP as determined by an enzyme-linked immunosorbent assay, indicating that compact morphology does not exclude encapsulation. The majority of the strains (11/12) showed autoagglutination in the salt aggregation test. The serotype 336 accounted for 46.7% of the isolates followed by serotype 5 (12.1%) and serotype 8 (12.1%). Particularly, twenty-six (24.3%) isolates reacted with two serotypes; 7 for type 8/336 and 19 for type 5/336. Five isolates (4.7%) were nontypeable with monoclonal antibodies specific for CP serotype 5, 8, or 336. The CP concentration in culture supernatants varied with the serotypes, and the total amount of CP produced by cells grown in a liquid medium was much less than that produced by cells grown on a solid medium. The Western blotting indicated that the CP bands of S. aureus serotype 5 and 8 were ranged in the molecular mass of 58-84 kilodalton (kDa), with additional bands in the region of approximately >or= 48 or Agglutination Tests ; Animals ; Bacterial Vaccines/administration & dosage ; Cattle ; Cattle Diseases/immunology/microbiology ; Female ; Mastitis, Bovine/*microbiology ; Polysaccharides, Bacterial/*classification/isolation & purification ; Serotyping/methods/veterinary ; Staphylococcal Infections/immunology/*veterinary ; Staphylococcus aureus/*classification/*immunology/isolation & purification

PURPOSE: The aim of this study was to demonstrate that the combined systemic administration of apomorphine (APO) and sildenafil have a synergistic effect on the erection in conscious rabbits. MATERIALS AND METHODS: The erections of the male New Zealand White rabbits (2-3kg, n=12) were assessed by measuring the length of the uncovered penile mucosa (LUPM) and duration of the erection, both before and after the intravenous administration of agents. After the injection of APO (0, 0.05, 0.1, 0.4mg/kg), sildenafil was administered intravenously in a dose-response manner (0.5, 1, 5mg/kg), followed by measurements for 0-120 minutes. In additional experiments, the effect of increasing the dosages of sildenafil, combined with APO, on blood pressure were evaluated. RESULTS: The intravenous administration of sildenafil caused a concentration dependent increase in the LUPM. There was a statistically significant increase in the LUPM with the administration of APO compared with no administration. The dosages of sildenafil and APO showing the greatest efficacy of sildenafil potentiation were 1mg/kg and 0.1 mg/kg, respectively. The intravenous administration of APO at a dose of 0.1mg/kg was more effective than those of 0.05 and 0.4mg/kg, with dosages of sildenafil of 0.5 and 1mg/kg. There was no additional increase in the duration of erection on the administration of APO. The intravenous administration of sildenafil caused a concentration dependent decrease in blood pressure, but there was no additional decrease on administration of the APO. CONCLUSIONS: We have shown that apomorphine elicits a stronger response on the erection induced by sildenafil in conscious rabbits, with no additional decrease in blood pressure.
Administration, Intravenous ; Apomorphine* ; Blood Pressure ; Humans ; Male ; Mucous Membrane ; Penile Erection* ; Rabbits* ; Sildenafil Citrate

To evaluate the effects of reflected glare on eyes, authors assessed the periodic health examination(1991-1996) and self-administered questionnaire data of a semiconductor producing company. The 13 lead frame(LF) department workers have been exposed to high reflected glare in the process for examining the silver plating products. We compared the data of connector(14) and clerical(12) workers as the non-exposed ones. Among LF department workers, 100% felt their visual acuity worsened(connector:81.8%, clerical:85.7%), and 70% had worn the glasses or contact lenses(connector :14.3 %, clerical :40%) since working at the company. Major ophthalmic symptoms of the LF workers are asthenopia, tearing, decreased visual acuity, congestion and glare. Decrease of visual acuity by Hahn's line change of the LF workers is significantly high during a 5-year follow-up(p<0.05 by simple regression) compared with connector, clerical workers.
Asthenopia ; Estrogens, Conjugated (USP) ; Eyeglasses ; Female* ; Glare ; Glass ; Humans ; Surveys and Questionnaires ; Semiconductors ; Silver* ; Tears ; Visual Acuity*

A case of resected primary duodenal cancer associated with early gastric cancer is reported. A 67-year-old male complaining of nausea and vomiting was admitted. Endo-scopic examination showed an ulcerative lesion withblood clot in the angle of the stomach and stricture in the duodenum. The stomach lesion was proven to be signet ring cell carcinoma by biopsy. Exploratory laparotomy was done and frozen specimen of duodenum was revealed to be malignant. Subtotal gastrectomy and pancreaticoduodenectomy with lymph node dissection were done and the specimen from stricture of duodenum was proven to be adenocarcinoma. The gastric cancer was limited to the mucosa and metastasis was not recognized in regional lymph node, Furthermore, there was normal mucosa between gastric cancer and duodenal cancer. Therefore, cancers in the stomach and duodenum were considered to be a case of double prim~iary cancer.
Adenocarcinoma ; Aged ; Biopsy ; Carcinoma, Signet Ring Cell ; Constriction, Pathologic ; Duodenal Neoplasms* ; Duodenum ; Esophagus* ; Gastrectomy ; Humans ; Intestines* ; Laparotomy ; Lymph Node Excision ; Lymph Nodes ; Male ; Mucous Membrane ; Nausea ; Neoplasm Metastasis ; Pancreaticoduodenectomy ; Stomach Neoplasms* ; Stomach* ; Ulcer ; Vomiting

Phantom limb pain is a painful sensation from an absent limb. The onset of pain is generally early, with 75% of patients developing pain within the first few days after amputation. The frequency and duration of attacks tend to be reduced with time, although the prevalence and intensity remain constant. We report here a case of a 38-year-old man who exhibited the signs and symptoms of phantom limb pain due to the above-knee amputations of both legs. He was not responded to opioid therapy and a continuous intravenous infusion of ketamine, an N-methyl-D-aspatate receptor antagonist, reduced his severe pain.
Adult ; Amputation ; Extremities ; Humans ; Infusions, Intravenous* ; Ketamine* ; Leg ; Phantom Limb* ; Prevalence ; Sensation

Meigs' sydrome is a relatively uncommon and the unusual condition of ascites and fluid in the chest associated with benign and solid tumors of the ovary with the gross appearance of a fibroma. The mechanism of this syndrome is not completely understood, but various lymphatics through the diaphragm seem the likely route for ascitic fluid into the chest. We present a case of Meigs' syndrome associated with fibrothecoma and elevate CA 125 with the brief review of literature.
Ascites ; Ascitic Fluid ; Diaphragm ; Female ; Fibroma ; Meigs Syndrome ; Ovary ; Thorax

No abstract available.
Carcinoma, Small Cell* ; Female ; Ovary*

PURPOSE: This study examined the histological changes and apoptosis in contralateral rat testes after testicular torsion or the repair of torsion, and investigated the functional role of clusterin in the pathogenesis for the injury of the contralateral testes after testicular torsion. MATERIALS AND METHODS: H&E staining and TUNEL assay were performed in contralateral testes following torsion or repair of torsion. The mean numbers of spermatids per tubule and apoptotic germ cells per tubule were calculated in each group with respect to time. The expression of clusterin after torsion or the repair of torsion was observed by RT-PCR and Western blot analysis. RESULTS: Following the repair of torsion, significant germ cell injuries and abnormal spermatogenesis were observed in the contralateral testes compared to those without repair. The expressions of clusterin mRNA and protein after testicular torsion were no different from those in the control testes. However, in the contralateral testes, the clusterin mRNA and clusterin protein expressions in the contralateral testes were increased 8 hours after the repair of torsion. The appearance of apoptosis in the contralateral testes after the repair of torsion appeared to correlate with the clusterin protein expression. CONCLUSIONS: The injury to contralateral testes during testicular torsion seems to be caused by the apoptosis induced by reperfusion of ischemic torsioned testis. Clusterin appears to play an antiapoptotic role in the apoptotic process in the injury to the contralateral testis due to the repair of testicular torsion.
Animals ; Apoptosis* ; Blotting, Western ; Clusterin* ; Germ Cells ; In Situ Nick-End Labeling ; Rats ; Reperfusion ; RNA, Messenger ; Spermatic Cord Torsion* ; Spermatids ; Spermatogenesis ; Testis

PURPOSE: Inhalational therapy is now one of the major method in managing asthma. Nebulizer is widely used at home nowadays, but there has been no survey on the usage of home nebulizer in Korea yet. This study was performed using questionnaires to evaluate that how the asthmatic patients use the personal nebulizer properly as an treatment tool for asthma. METHODS: Forty-six asthmatic children living in Chunlabukdo area in Korea who use the personoa nebulizer at home from October 1999 to March 2000 were enrolled. Personal history, purpose of personal nebulizer, understanding of the instruction to use were analyzed using questionnaires. RESULTS: 1) The sex ratio was not statistically significant (M : F ratio was 1.3 : 1) and the mean age was 3.7 years. 2) Thirty-seven cases (80.4%) purchased nebulizer by doctor's recommendation. 6 cases (13.1%) by personal need without doctor's recommendation and 3 cases (6.5%) by acquaintances' recommendation. 47.8% of patients purchased through small-scale suppliers. 32.6% purchased through hospital, and 19.6% through large nebulizer manufacturers. 3) Sixty-three percents of patients had an explanations about the basic instructions to use the nebulizer. Explanations on maintenance were given only in 19.6% of total and was mostly given through large nebulizer manufacturers. 4) Proper washing with sterilization took place in 54.3%, replacement of kits and air filters were done in only 15.2% of the total, but nobody was recommended annual check up. 5) There was no difference in the techniques of using nebulizers by the parents' level of knowledge about asthma. CONCLUSION: Many problems on the usage of personal home nebulizer in asthmatic children were revealed by this study. Education of patients on how to use and manage nebulizers is necessary when prescribe or sell the nebulizers.
Air Filters ; Asthma ; Child* ; Humans ; Korea ; Nebulizers and Vaporizers* ; Patient Education as Topic ; Surveys and Questionnaires ; Sex Ratio ; Sterilization