Adult ; Child, Preschool ; Female ; Humans ; Ichthyosis Vulgaris ; genetics ; Infant ; Male ; Mutation ; Pedigree

Objective To investigate current knowledge levels of medical staff for hypoglycemia care process in endocrinology department and provide basis for related knowledge training and interventions. Methods A total of 119 medical workers were recruited and investigated using self-made hypoglycemia care process questionnaire via cross-sectional survey.Results The correct rates in knowledge of carbohydrate foods number, first retest time after eating, treatments for patients who were not recover after first eating and retest time of patients who were recover after eating were low (<70%). The correct rates of first retest time after eating in nurses (75.9%, 55.6%) were better than doctors (42.2%, 22.2%). The correct rates of first retest time after eating and treatments for patients who were not recover after first eating were significantly different among patients with individual degrees (P<0.05).Conclusions The knowledge levels of medical staff for hypoglycemia care process in endocrinology department are not high and different among individuals. The training of hypoglycemia care process is necessary.

Hypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. Benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay. With BD treatment, the osteogenic differentiation and maturation of MC3T3-E1 cells were increased, as established by the alkaline phosphatase (ALP) activity test, matrix mineralized nodules formation, osteogenic genetic test, and protein expression analyses. Moreover, our data showed that the BMP2/Smad pathway could be the partial mechanism for the promotion of osteogenesis by BD, while BD might suppress the possible function of osteoclasts through the OPG/RANKL/RANK (receptor activator of nuclear factor-κB (NF-κB)) pathway. The hypothesis that BD bears a considerable potential in further research on its dual therapeutic effect on hypertensive patients with poor skeletal conditions was proved within the limitations of the present study.