Objective: To establish an HPLC method to detect the limit of 5-hydroxymethylfurfural in Yueju Baohe pills.
Methods: A Zafex Acutfex PW-C₁₈ column（4.6 mm×250 mm, 5 μm） was adopted with mobile phase consisted of acetonitrile-0.1% formic acid aqueous solution（2:98）at the flow rate of 1.0 mL·min^-1. The column temperature was 40 ℃ and the detective wavelength was set at 284 nm.
Results: The linear range of 5-hydroxymethylfurfural was 0.491 9-122.98 μg·mL^-1 (r=1.000). The average recovery(n=6) was 97.99% with RSD 0.61%. All of the RSDs for precision,repeatability and stability met the methodological requirements. According to the proposed limit, 17 of the 65 batches of samples did not meet the requirements, and the failure rate was 26.15%.
Conclusion: The established method is suitable for the limit test of 5-hydroxymethylfurfural in Yueju Baohe pills.There was a significant difference in the content of 65 batches of samples. It is necessary to investigate the limit of 5-hydroxymethylfurfural in Yueju Baohe pills.

"Disease-syndromes-formulas-medicines" binding mode is benefit to reveal the scientific connotation of the compatibility and mechanism of classical prescriptions of traditional Chinese medicine (TCM). In this article, Zuojin Wan was used as a model prescription, with the etiology and pathogenesis of liver-fire invading stomach in TCM, and modern pathological and pharmacological interpretation, the compatibility, nature-flavour, channel tropism, the active substance and pharmacology of Zuojin Wan were explained, in order to reveal the compatibility, nature-flavour and channel tropism of Zuojin Wan, and to provide ideas for the development of modern compound prescription.
Drug Compounding ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; therapeutic use ; Gastrointestinal Diseases ; drug therapy ; etiology ; Humans ; Liver Diseases ; drug therapy ; etiology ; Medicine, Chinese Traditional