Objective To explore the effects ofDanshen Tongluo Detoxication Decoction on the inflammatory response of rats with bone marrow stem cell (BMSC) transplantation in acute myocardial infarction (AMI); To discuss its mechanism of action.Methods The whole bone marrow adherent method was adopted for BMSCs separation and culture. The AMI model was established by closing the left anterior descending coronary artery of SD rats. After the modeling, SD rats were randomly divided into sham-operation group, model group and BMSCs group (BMSCs transplantation group),Danshen Tongluo Detoxication Decoction group, Danshen Tongluo Detoxication Decoction + BMSCs group, 10 rats in each group. BMSCs cell suspension was injected directly into the edge of the myocardial tissue infarction area; Chinese medicine or normal saline were administered for gavage. 4 weeks later, the contents of MCP-1 and sICAM-1 in each group were detected by ELISA. TLR-4 expression was measured by Western blot method, and HE staining was used to observe the myocardial tissue pathological changes.Results Compared with the model group, the contents of MCP-1 and sICAM-1 and the protein expression of TLR-4 in BMSCs group,Danshen Tongluo Detoxication Decoction group, andDanshen Tongluo Detoxication Decoction + BMSCs group decreased, Danshen Tongluo Detoxication Decoction group was better than BMSCs group (P<0.05,P<0.01), andDanshen Tongluo Detoxication Decoction + BMSCs group was better than BMSCs group andDanshen Tongluo Detoxication Decoction group(P<0.05,P<0.01).Conclusion BMSCs transplantation combined withDanshen Tongluo Detoxication Decoction can restrain the inflammatory response of AMI model rats and repair ischemic myocardium issue, which mechanism may be related to regulating TLR-4 induced inflammatory response.

The study on etiology and pathogenesis of coronary heart disease / thoracic obstruction has been carried on for a long time, and the understanding of TCM concept of blood stasis, heat and toxin was deeper and wider.A large quantity of clinical trials have proved that one of key pathogenesis of coronary heart disease / thoracic obstruction is the toxin induced by accumulation of blood stasis and heat, which damages heart blood or nutrient.In detail, the evil of blood stasis and heat were blocked or accumulated in human bodies to generate the toxic materials called toxin, which can damage collaterals of heart, burn out nutriment-blood of heart, block vessels of heart, and in the end, cause coronary heart disease / thoracic obstruction.Undoubtedly, the therapy of activating blood, clearing heat from the nutritive level, removing toxic substances has positive effectiveness.This article explains the relationship between the evil of blood stasis, heat and toxin with the occurrence and progress of coronary heart disease / thoracic obstruction, and discuss the theoretical basis of TCM therapy mentioned above directed at the evils.