1.Effects of culture medium osmolarity on the proliferation of human hepatoma cells
Jing TIAN ; Yu WANG ; Wenge TONG ; Chenhao ZHANG ;
Journal of Medical Postgraduates 2003;0(07):-
Objective: To investigate the effects of culture medium osmolarity on the proliferation of human hepatoma cell line HHCC. Methods:Cell proliferation was analyzed by MTT assay and cell cycle analysis was carried out by flow cytometry. Results:Hyperosmolarity decreased significantly the absorbance (A) of HHCC cells and cell number, and hyposmolarity have counter effects. Compared with control, hyperosmolarity blocked cell cycle progression at the G 1 phase, while hyposmolarity accelerated cells into S phase from G 1 phase, and the effect of hyposmolarity was weakened by chloride channel blocker NFA. Conclusion:Culture medium osmolarity influenced the proliferation of HHCC cells by mediating cell cycle.
3.Management and operation of extra-large Fangcang hospitals: experience and lessons from containing the highly contagious SARS-CoV-2 Omicron in Shanghai, China.
Yun XIAN ; Chenhao YU ; Minjie CHEN ; Lin ZHANG ; Xinyi ZHENG ; Shijian LI ; Erzhen CHEN ; Zhongwan CHEN ; Weihua CHEN ; Chaoying WANG ; Qingrong XU ; Tao HAN ; Weidong YE ; Wenyi XU ; Xu ZHUANG ; Yu ZHENG ; Min CHEN ; Jun QIN ; Yu FENG ; Shun WEI ; Yiling FAN ; Zhiruo ZHANG ; Junhua ZHENG
Frontiers of Medicine 2023;17(1):165-171
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