Objective To observe the effects of Er-YAG and surgical resection in the treatment of oral cav-ity mucous cyst. Methods A total of 63 patients with oral cavity mucous cyst ,selected in our hospital from March 2013 to May 2014,were divided into control group(n=31)and experimental group(n=32)based on dif-ferent therapies. The oral cavity mucous cysts in the experimental group and the control group were treated with Er-YAG and surgical resection ,respectively. The two groups were compared in terms of therapeutic effect. Results The cure rate in the two groups had no significant difference (P>0.05). The operation time in the experiment group was significantly shorter than the control group(P<0.05). In the experiment group,the incidence of intra-operative blood loss,postoperative edema and postoperative scarring were significantly lower than those of the con-trol group(P<0.05),but the two-year recurrence rate in the two groups had no significant difference(P>0.05). The SAS scores had no significant difference between the two groups at 1 week after operation (P > 0.05),but they were significantly higher in the experiment group than in the control group before operation and 3 days after op-eration(P < 0.05). In HAD emotional determination,the numbers of patients who had the manifestation of anxi-ety and depression in the experiment group were smaller than control group before operation and 3 days after opera-tion(P<0.05),but there was no significantly difference between the two groups at 1 week after operation(P >0.05). Conclusions The curative effect of Er-YAG in the treatment of oral mucous cysts is close to that by the conventional surgery,but the former is advantageous for easier operation,less postoperative complications and alle-viation of patientspsychology pressure,worthy of clinical spreading.

With the development of modern vitrectomy,some new ideas have been proposed concerning the treatment of Terson syndrome.Developed neurosurgery has offered more chances of survival to patients with subarachnoid hemorrhage(SAH).Terson syndrome,as the initial presentation,has gained more and more attention from ophthalmologists,though strict guidelines for its treatment have not yet been established.The paper reviews the progress in the clinical researches of Terson syndrome.

A single factor duplicate test was designed to investigate whether bovine recombinded resistin impacts the expression of pyruvate carboxylase (PC) mRNA and the activity of PC in vitro culture bovine hepatocyte.Bovine recombinded resistin was added to the media with 0,25,50,100,200 and 400 ng/L.Abundance of PC mRNA in bovine hepatocyte,which was cultured with bovine recombinded resistin for 12 hours,was determined by real-time fluorescence quantitative RT-PCR,and activity of PC was determined by colourimetry.The results showed that bovine recombinded resistin could downregulate the expression of PC mRNA and the activity of PC in vitro culture bovine hepatocyte.

The inhibitory effects of diallyl sulfide (DAS) derived from allicin on in vitro and in vivo proliferation of human osteosarcoma MG-63 cells and the action mechanism, and the influence of DAS on invasive capability of MG-63 cells were investigated in order to search for the novel medicines for osteosarcoma. In the in vitro experiment, MG-63 cells were treated with different concentrations of DSA, and the morphological changes of MG-63 cells were observed under an inverted phase microscope. MTT method was used to assay the proliferation of MG-63 cells. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the VEGF mRNA expression level in MG-63 cells. By using Transwell invasion assay, the influence of DAS on invasive ability of MG-63 cells was tested. In the in vivo experiment, the nude mice MG-63 cells tumor-bearing model was established, and different concentrations of DAS were injected beside the tumor. Twenty-one days after treatment, the mice were killed, the tumor size and tumor inhibition rate were calculated. The microvessel density (MVD) was determined by using immunohistochemistry. In the in vitro experiment, different concentrations of DAS could obviously inhibit proliferation of MG-63 cells in a time- and concentration-dependent manner. RT-PCR revealed that the expression levels of VEGF mRNA in DSA groups (different concentrations) were significant reduced as compared with those in control group (all P<0.05). Transwell invasion assay indicated that in 20 and 40 μg/mL DAS groups, the number of migratory cells was 91.4±8.3 and 81.8±7.4 respectively, which was significantly declined as compared with that in control group (150.4±14.7, both P<0.05). In the in vivo experiment, DAS could significantly suppress the growth of MG-63 tumor-bearing tissue. Immunohistochemistry demonstrated that different concentrations (20 and 40 μg/mL) of DAS could significantly decrease MVD of MG-63 tumor-bearing tissue (all P<0.05). It was suggested that DAS could inhibit the growth of MG-63 cells probably by suppressing the expression of VEGF mRNA.

Objective: To describe the degree of poverty caused by diseases among farmers who have joined New Rural Cooperative Medical Scheme( NRCMS) in Anhui province, and analyze the ability of NRCMS to reduce health risk factors in Anhui province, in order to provide suggestions for improving the NRCMS strategy. Methods: This paper randomly selects three counties to conduct comparative analysis on the NRCMS strategic changes from the aspects of registration rates, funding levels and compensation ratios between 2013 and 2014, in order to analyze the ability of NRCMS to reduce health risk factors from the aspects of the rate of poverty caused by diseases, the resolving degree of poverty caused by diseases, the alleviation degree of poverty caused by diseases, etc. Results: The ability of NRCMS to reduce health risk factors in Anhui province has improved between 2013 and 2014 , but the improve-ment is limited. Conclusions:In order to reduce the incidence of poverty caused by diseases among the farmers, gov-ernment should reform the payment methods, control the outflow of patients and improve the multi-level security sys-tem, in order to gradually reduce the poverty caused by diseases among farmers.

OBJECTIVE To investigate the distribution features and clinical significance of allergens in patients with chronic rhinosinusitis(CRS). METHODS A retrospective analysis was performed in 136 cases of CRS. Allergen features were compared in the two subtypes of CRS: chronic rhinosinusitis with nasal polyps(CRSwNP) and chronic rhinosinusitis without nasal polyps(CRSsNP). Differences of allergens features of CRS were analyzed, and they were compared to the allergens of 36 patients with allergic rhinitis(AR). RESULTS The total allergen positive rate in CRS was 48.5％. The allergen positive rate, distribution proportion of inhaled and food allergens, distribution proportion of allergens subsets, distribution proportion of single and multiple allergens between two subtypes of CRS had no significant difference(P >0.05). The major allergens of CRS were single inhaled allergens(84.8％). Some CRS patients were allergic to food allergen(9.1％). The minority allergens of CRS were a mixture of inhaled and food allergens(6.1％). Compared to AR, the distribution proportion of inhaled and food allergens(χ2=14.801, P =0.001), the distribution proportion of allergens subsets(χ2=12.951, P=0.005), and the distribution proportion of single and multiple allergens(χ2=9.067, P=0.003) had significant difference. CONCLUSION The allergen positive rate of CRS is much higher than the prevalence of allergic diseases in general population, suggesting that allergic factors may be closely correlated to the pathogenesis of CRS. The clinical features of allergens are similar in the two subtypes of CRS, while there are significant differences in allergen distribution between CRS and AR patients. The detection of allergens may be helpful in prevention and treatment of CRS.

Objective To investigate the difference between single lumen endotracheal intubation of thoracoscope and traditional double lumen endotracheal intubation of thoracoscope in process of thymectomy. Methods From January 2010 to June 2014, clinical data of 30 cases with thymectomy under single lumen endotracheal intubation of thoracoscope (group A) and 30 cases with thymectomy under traditional double lumen endotracheal intubation thora-coscope (group B) were analyzed. Results There were no death patients in both groups. Group A:endotracheal intu-bation time (2.67 ± 0.72) min, surgery time (48.37 ± 4.64) min, the bleeding (26.17 ± 9.62) ml; Group B:endotracheal intubation time (5.55 ± 0.71) min, surgery time (52.10 ± 5.68) min, the bleeding (33.00 ± 7.94) ml. Conclusion Compared with traditional double lumen endotracheal intubation under thoracoscope, the single lumen endotracheal intubation of thoracoscope showed that intubation time was significantly shorter, and reduced the oc-currence of postoperative complications, the operative field was exposed more completely, reduced operation time and blood loss.

This study examined the effects of ω-3 polyunsaturated fatty acid (ω-3PUFA) on the expression of toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and some related inflammatory factors in peripheral blood mononuclear cells (PBMCs) of patients with early-stage severe multiple trauma. Thirty-two patients who were admitted to the Department of Traumatic Surgery, Tongji Hospital (Wuhan, China) between May 2010 and November 2010, and diagnosed as having severe multiple trauma with a injury severity score (ISS) no less than 16, were enrolled in the study and divided into two groups at random (n=16 in each): ω-3PUFA group and control group in which routine parenteral nutrition supplemented with ω-3PUFA or not was administered to the patients in two groups for consecutive 7 days. Peripheral blood from these patients was collected within 2 h of admission (day 0), and 1, 3, 5 and 7 days after the nutritional support. PBMCs were isolated and used for detection of the mRNA and protein expression of TLR2 and TLR4 by using real-time PCR and flow cytometry respectively, the levels of NF-κB by quantum dots-based immunofluorescence assay, the levels of TNF-α, IL-2, IL-6 and COX-2 by ELISA, respectively. The results showed that the mRNA and protein expression of TLR2 and TLR4 in PBMCs was significantly lower in ω-3PUFA group than in control group 5 and 7 days after nutrition support (both P<0.05). The levels of TNF-α, IL-2, IL-6 and COX-2 were found to be substantially decreased in PBMCs in ω-3PUFA group as compared with control group at 5th and 7th day (P<0.05 for all). It was concluded that ω-3PUFA can remarkably decrease the expression of TLR2, TLR4 and some related inflammatory factors in NF-κB signaling pathway in PBMCs of patients with severe multiple trauma, which suggests that ω-3PUFA may suppress the excessive inflammatory response meditated by the TLRs/NF-κB signaling pathway.

Most of the current experiments are obsolete and verified,the students are not interested in and the experiments are out of touch with the actual work.These are the common problems in the experiment teaching of occupational health and occupational medicine.By taking the students who majored in preventive medicine as the subjects,this study aimed to explore the effects of independent designed experiment teaching mode,which was based on dividing the groups before class,choosing the projects by students themselves,deciding the design of the program via the discussion of teacher and students and doing the experimental reports and sharing the experience.The results showed that,in the premise of the preliminary master of the theory and basic skills of occupational health and occupational medicine,carrying out independent designed experiments in the last three weeks of semester,to a certain extent,could arouse students' interest in learning,cultivate their abilities of independent thinking,practice,problem analysis and solution,and team cooperation.However,restricted by lack of teachers,inadequate equipment and high cost and other factors,this teaching mode is only suitable for small class.

Objective To investigate the incidence, clinical symptoms, correlative risk factors and prognosis of dysautonomia in patients with severe traumatic brain injury. Methods A total of 142patients with severe traumatic brain injury treated from January 2008 to March 2010 were retrospectively surveyed to compare the clinical features of dysautonomia group and control group. Logistic regression was used to analyze the risk factors for dysautonomia. At 6 months post-trauma, the Glasgow Outcome Score (GOS) was used to measure the outcome. Results Of all the patients, 94 patients survived and were followed up. There were 16 patients ( 17% ) diagnosed as dysautonomia depended on clinical symptoms,with statistical difference in aspects of GCS, coma duration, ICU time and average length of stay (ALOS)(P < 0.05). The patients with dysautonomia tended to have poorer outcome ( P < 0.05 ) and showed a positive association with diffuse axonal injury (DAI) ( OR = 11. 25, CI 7.65-16.54 ). Conclusion Dysautonomia has high incidence and is usually severe in patients with severe traumatic brain injury,when DAI may contribute to its occurrence and result in poor prognosis.