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Objective To Cenderstand the sanitation status of secondary drinking water supply system of public places. Methoods The sanitation of secondary drinking water supply system was investigated by on_site survey method and the sanitary quality of water samples were determined. Results The over_standard rates contents of Fe and total coliformz of water samples of secondary water supply system significantly increased compared with the related levels of tap water of municipal water supply. The unqualified rate of free residual chorine of water sample from secondary water supply system reached 54.55%. Conclusions The equipments for secondary water supply were obsolete and unretional design. The sanitary quality of secondary water supply was also affected by geological structure easily.

Objective To work out a proper treatment regimen for hypertension in elderly patients with type 2 diabetes mellitus. Methods Sixty-five elderly patients 〔aged (68.3?4.4) yrs old〕 with hypertension and type 2 diabetes were randomized into three arms of antihypertensive treatment: arm A, n=20, treated with nifedipine in long-acting gastrointestinal-transport system (nifedipine-GITS) 60 mg/d; arm B, n=22, treated with nifedipine-GITS 30 mg/d and valsartan (angiotensin Ⅱ receptor blocker, ARB) 80mg/d; and arm C, n=23, treated with nifedipine-GITS 30mg/d, Valsartan 80mg/d and sustained-releasing indapamide (diuretics)1.5 mg/d. Results Eight weeks later, both systolic blood pressure (SBP) and diastolic blood pressure (DBP) were decreased significantly in all of the patients ( P

AIM: To study the influence of arachidonic acid(AA) on the action potential and L-type calcium current in rabbit cardiomyocytes.METHODS: Single ventricular myocyte was isolated using enzyme dispersion method.Whole-cell clamp-patch technique was used to record action potential and L-type calcium current.RESULTS: ① AA shortened action potential duration obviously,without marked effect on the resting potential and action potential amplitude.② AA reduced the current densities from(10.21?3.15)PA/PF to(6.53?2.17)PA/PF(n=6,P

Ischemic heart disease (IHD) is a serious threat to health of the elderly,and endothelial progenitor cells therapy-induced therapeutic angiogenesis can improve vascular reperfusion and functional recovery in ischemic myocardium.Endothelial progenitor cell (EPC) therapy plays an important role in endothelial repair and postnatal angiogenesis.And the concept of EPC therapy has received attention.EPC population is decreased in numbers and in functional activity,which are correlated with age and cardiovascular risk factor.And patients with these backgrounds have less therapeutic benefit.In this review,we will focus on overview of the latest research progressions of characteristic of EPC in the elderly with IHD,and on current studies of EPC-based angiogenesis therapies for providing theoretical basis in the promising clinical treatment.

Objective To build clinical nursing pathway for artificial abortion operation and explore the effect of this method. Methods The nursing pathway was designed and carried out. The satisfaction degree with nursing, status of patients' awareness about contraception and artificial abortion operation was compared before and after the nursing pathway was implemented. Results Before and after the nursing pathway was implemented,the proportion of missing item of operation record was 0.636 and 0.013, the satisfaction degree with nursing was 77.0% and 92.3%, rate of patients' awareness about contraception and artificial abortion operation was 47.6% and 96.1%. Conclusions The clinical nursing pathway for artificial abortion operation may improve the degree of satisfaction- and the status of patients' awareness about contraception and artificial abortion operation, and decline the proportion of missing item of operation note, it ensures high nursing quality and safety surgery, which is worthy of popularizing.

Objective To study the influence of arachidonic acid (AA) on L-type calcium channel in rabbits sin-gle cardiomyocyte and its mechanism of antiarrhythmia. Method The single ventricular cardiomyocyte was isolat-ed by using enzyme dispersion method and whole-cell clamp-patch technique was used to record L-type calcium current.All data were analyzed using ANOVA. Results AA inhibited Ica-L in a concentration-dependent manner. The application of 3 μmol/L, 10 μmol/L and 20 μmol/L arachidonic acid reduced the density of peak Ica-L from (10.79±0.93)pA/pF to (8.99±0.43)pA/pF to (7.60±0.35)pA/pF and to (5.60±0.30)pA/pF, respctive-ly (n=7, P<0. O1 ). The Ica-Lpartially resumed after washout. The AA up-shifted the I-V curves of Ica-L without changes of their shape,peak and reverse potentials. The AA also markedly shifted the inactivation curve to left, and prolonged the recorvery time from inactivation,but did not change the curve of calcium channel activation. Con-clustions By acceleration of L-type calcium channel inactivation and prolongation of recorvery time from inactiva-fion,arachidonic acid can reduce the calcium ion influe and prolong effective refractory period, playing the role of antiarrhythmia.

Objective To investigate changes and clinical significance of serum granulocyte-macrophage colony stimulating factor(GM-CSF),tumor necrosis factor-alpha(TNF-?),interleukin-6(IL-6)and interleukin-8(IL-8)level in patients with bee-sting poisoning.Methods One hundred and nine patients with bee-sting poisoning were also divided into slight poisoning group,moderate poisoning group,and server poisoning group and also divided into dead group and survival group according to the prognosis.Thirty healthy persons were selected as control group.GM-CSF and TNF-?were measured using radioimmunoassay,IL-6 and IL-8 was measured using enzyme linked immunoadsorbent assay(ELISA),and they were compared with control grouup.Results The levels of serum GM-CSF,TNF-?,IL-6,IL-8 in slight poisoning group were increased a little,and there were no significant difference compared with control group(P＞0. 05).The levels of serum GM-CSF,TNF-?,IL-6 and IL-8 were significantly higher in moderate poisoning group and severe poisoning group than those in the control group(P＜0.01),but the severe poisoning group were much higher than the moderate poisoning group(P＜0.01).They were significantly higher in survival group than those in dead group(P＜0.01).APACHEⅡscores were obvious correlated with the levels of serum GM-CSF,TNF-?,IL-6 and IL-8(P＜0.05 or P＜0.01). Conclusion GM-CSF,TNF-?,IL-6 and IL-8 may participate in the occurrence and progress of patients with bee-sting poisoning.Dynamic monitoring of serum GM-CSF,TNF-?,IL-6,IL-8 and APACHEⅡscores plays an important role in diagnosis of the severity of patients with bee-sting poisoning and its prognosis.The use of antagonist and inhibitor of cytokines may be helpful to treat patients with bee-sting poisoning.

Objective To explore the effect of polychlorinated biphenyls (PCBs) on the learning and memory of gestational and lactational exposure to polychlorinated biphenyls in rats offspring.Methods The PCB mixture (A1254,0,5,10,20 mg/kg body weight) was administered to pregnant wistar rats every 3 days by gavage from gestational day (GD) 5 to postnatal day (PND) 20.To assess the effects on offspring following such exposure,Morris water maze test was performed to assess the learning and memory ability.Calcium concentration was assayed in hippocampus and the ultramicro structure was observed.Results After training for four days,the escaping latency in every group decreased significantly compared with the first day,especially the control group,the results in the fourth day decreased significantly with the early three days(P＜0.05).The offsprings of the 10 and 20 mg/kg had prolonged time of passing the aim target ((5.23± 1.16) s,(7.90±3.21) s,(11.74±6.56) s and (20.83± 8.38) s ; P＜0.05),decreased number of crossing platform ((4.14± 1.21),(3.00± 1.32),(2.65± 1.13),(2.42± 1.31) ; P＜0.05) and swimming time in the target area ((40.14±7.14)s,(33.76±5.58)s,(32.45±6.00)s and (30.63±5.10) s; P＜0.05)compared with those of the rats of control and 5 mg/kg groups.The calcium concentration increased ((121.16± 12.23) nM,(141.27±24.66) nM,(163.32±29.75) nM,(261.46±27.79) nM) and the ultramicro structure in hippocampus changed obviously in the high exposure group.Conclusion Gestational and lactational exposure to aroclor 1254 in rats could affect the leaming and memory ability of offsprings.

AIM: To develope a reversed-phase high performance liquid chromatographic method for the quantitative determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection. METHODS: The operation was carried out in the Kromasil ODS column with the mobile phase consisting of a mixture of water-methanol-acetonitril aceti acid(95∶10∶5∶2,v/v),in which the flow rate of 1.0 mL/min and UV detection wavelength at 326 nm were set to determine the contents of chlorogenic acid and caffeic acid. RESULTS: There were good linear relations between the concentrations and the peak-areas of chlorogenic acid and caffeic acid.The relative standard deviation of peak areas for chlorogenic acid or caffeic acid were 0.60%,0.32%,respectively.The two kinds of standard solutions were both stable in 16 h(RSD=0.54% for CGA,0.11% for CFA).The average recovery was 100.1%,99.6% for CGA,CFA,repectively.The detectable limit(S/N=3)was 0.012,0.020 mg/L,and the quantitative limit (S/N=10) was 0.037,0.052 mg/L,repectively. CONCLUSION:The method is simple,sensitive,rapid and accurate,and can be used for the quality control of Shegan Kangbingdu Injection