Objective To observe the clinical effect of treating atlanto-axial subluxation by manipulative reduction therapy. Methods 128 patients with atlanto-axial subluxation confirmed by cervical plain X ray were randomized into two groups: 64 in the treatment group treated by manipulative reduction therapy, and 64 in the control group treated by the routine cervical traction. The clinical effect was evaluated by both clinical and X-ray confirmation. Results The X-ray study showed that after treatment the reconstruction of excursion in odontoid process in the treatment group was better than that in the control group significantly (P<0.01). The improvement of vertebra basal artery blood flow in the treatment group was also better than that in the control group significantly (P<0.05). The total effective rate of the treatment group was 95.3%, while the control group was 78.1%. Conclusion The technique of manipulative reduction on atlanto-axial subluxation acts more directly on the cause of the disease than the routine traction, and exerts better results in restoring proper atlanto-axiai joint position and mechanics stability of cervical vertebra.. Thus it is the effective shortcut way with high safety and low cost.

Objective:To study the effects of hydroxyapatite(HA) coating on sandblasted and acid-etched(SLA) surface of titanium dental implants(HA-SLA-Ti) on the biologic characteristics of marrow-origin osteoblasts(MOOBs).Methods:Coating of HA on the SLA surface of titanium dental implants were formed by ion beam assisted deposition (IBAD) method. MOOBs,cultured in vitro,were seeded onto the surface of HA-SLA-Ti and SLA-Ti respectively, the growth of MOOBs on the two kinds of samples were observed by scaning electron microscope.The proliferation index, alkaline phosphatase(AKP) activity, osteocalcin(BGP) content of MOOBs and mRNA relative expression level of osteopontin(opn) were examined and compared between two groups.Results: MOOBs grew well on the surface of HA-SLA-Ti; the proliferation index, AKP activity and BGP content of MOOBs were higher in HA-SLA-Ti group than those in SLA-Ti group(P

Objective To study the causes of conversions to open surgery during laparoscopic cholecystectomy (LC). Methods The study was based on a retrospective review of 156 cases of conversions to laparotomy out of 12672 cases of LC from December 1991 to October 2001 in this hospital. Results Ninety-two (58.97%) cases of conversions were due to complicated clinical condition and preoperative misdiagnosis, 47 (30.13%) cases of conversions due to technological complications, and 17 (10 90%) cases underwent delayed laparotomy. The total conversion rate was 1.23% and the rate of bile duct injuries was 0.2% (26/12672). Conclusions Inadequate preparation, deficiency of experience, complicated clinical situation, and anatomical variation of bile ducts are the leading causes to conversions.

Objective To discuss clinical effects of combined use of duodenoscopy and laparoscopy in the treatment of acute billiary pancreatitis (ABP), and to establish a systematic protocol of minimally invasive treatment for ABP. Methods According to the patients’ biliary tract conditions, severity of disease and treatment methods, a total of 696 patients with ABP were given laparoscopic cholecystectomy (LC) alone, or endoscopic retrograde cholangiopancreatography (ERCP) and LC, or ERCP and laparoscopic common bile duct exploration (LCBDE), or ERCP and endoscopic sphincterotomy (EST), or individualized treatment for severe acute pancreatitis (SAP). Clinical effects were observed. Results Among the 696 patients, 330 patients (47.4%) received EST and biliary stones were successfully removed under endoscope in 267 patients (38.4%). ERCP, LC and LCBDE were conducted in 411 (59.1%), 513 (73.7%) and 85 (12.2%) patients, respectively, and successfully accomplished in 409 (99.5%), 511 (99.6%) and 82 (96.5%) patients, respectively. Out of 36 patients with SAP, 34 patients survived (94.4%). The total cure rate was 99.7% (694/696). Conclusions Combined use of duodenoscopy and laparoscopy is significantly effective for acute billiary pancreatitis and benefits the improvement and standardization of the protocol of minimally invasive treatment for acute billiary pancreatitis.

Objective:To study the effect of 5-FU polylactic acid nanospheres(5-FU-PAN)on the proliferation and apoptosis of the human tongue squamous cell carcinoma Tca83 cells.Methods:Tca83 cells were exposed to 5-FU-PAN or 5-FU at the doses(mmol/L)of 1?10-2,2?10-2 and 4?10-2 for 1,3,5,7,9 and 11 days respectively.The survival rates of the cells were examined by MTT assay,the apoptosis of the cells was observed by acridine orange fluorescent staining and the cell cycle distribution was studied by flow cytometry.Results:Both 5-FU-PAN and 5-FU inhibited the proliferation and promoted the apoptosis of the cells dose and time dependantly.5-FU-PAN showed stronger effects than 5-FU.The two agents blocked the cells at S phase and 5-FU-PAN was more potential than 5-FU.Conclusions:5-FU-PAN is more effective in the growth inhibition and apoptosis promotion of Tca83 cells.

BACKGROUND:It is an important issue of alveolar ridge preservation after tooth extraction. Because of the limited treatment and geographical conditions, lack of awareness of oral health, many local people in frontier areas in Xinjiang have poor alveolar ridge that is not of timely repair after tooth extraction. Thus, it is important to find a suitable local and efficient way to save the residual ridge, which has a more practical clinical value. OBJECTIVE:To investigate the feasibility of nano-colagen artificial bone used for alveolar ridge preservation in the Kazakh in Xinjiang Tacheng Region, China. METHODS: Sixty-eight Kazakh patients with bilateral extraction from Tacheng region, Xingjiang Uygur Autonomous Region, China were selected in a self-controled trial. According to the principle of a minimum alocation imbalance index, the experimental side and control side of extraction patients were confirmed. In the experimental side, nano-colagen artificial bone was implanted; while, conventional treatment was done in the control side. Multi-slice spiral CT was used to scan the regions of extraction interest to measure the relative gray value of alveolar bone mineral density immediately and 3 months after implantation. RESULTS AND CONCLUSION:Immediately after implantation, tooth extraction sockets were visible on CT images both in the experimental and control sides; but after 3 months, the extraction sockets became unclear on the CT images, and CT values were close to those of the surrounding alveolar process, but bone tissues were ful of the bone graft area in the experimental side. The alveolar bone mineral density was higher in the experimental side than the control side at 3 months after implantation. These findings indicate that the nano-colagen artificial bone has good clinical achievement in alveolar ridge preservation in the Kazakh in Xinjiang Tacheng Region.

BACKGROUND:Selection of proper bone transplantation materials to substitute autogenous bone for treatment of jaw bone defect in patients has become the focus of current researches.OBJECTIVE:To observe the ability of tissue engineered bone constructed with nano-hydroxyapatite/collagen/poly(L-lactic acid)(nHAC/PLA)co-cultured with bone marrow stromal cells(BMSCs)in treatment of rabbit mandibular defect.and to make a comparison with autogenous bone and simple nHAC/PLA.DESIGN.TIME AND SETTING:A randomized controlled animal experiment was performed at the Animal Laboratory of Jinzhou Central Hospital between March and October 2007.MATERIALS:A total of 40 New Zealand rabbits were randomized to 4 groups.with 10 rabbits per group:nHAC/PLA co-cultured with rabbit BMSCs(BMSCs/nHAC/PLA),autogenous bone,nHAC/PLA,and control.METHODS:A 15 mm×15 mm full-thickness bone defect was created in rabbit mandible body.In the BMSCs/nHAC/PLA group.bone defect was repaired by nHAC/PLA co-cultured with BMSCs for 14 days in vitro;the bone defect in the autogenous bone and nHAC/PLA groups was treated by autogenous iliac bone and nHAC/PLA.respectively;while the control group received no any treatment.MAIN OUTCOME MEASURES:At 1,3,and 6 months after surgery,bone mineral density(BMD)measurement and histological examination were performed to evaluate the repairing effects of bone defect.RESULTS:The BMSCs/nHAC/PLA group exhibited the BMD similar to the autogenous bone group(P＞0.05),but it was significantly higher compared to the nHAC/PLA group and the blank control group(P＜0.01).At 6 months after surgery.newly formed bone tissue grew towards large bulk and bridged defected stumps.and the seaffold materials were hardly seen in the BMSCs/nHAC/PLA and autogenous bone groups;simultaneously.the nHAC/PLA group exhibited increased trabecular bone with many fibrous tissues and incompletely degraded seaffold materials.CONCLUSION:BMSCs/nHAC/PLA seaffold exhibits the effects similar to autogenous bone.but superior to nHAC/PLA seaffold in the treatment of mandibular defects.

Objective:To study the feasibility of a new composite mineralized collagen membrane in alveolar ridge preservation.Methods:The third mandibular premolars on both sides were extracted from 12 dogs,the 24 alveolar sockets were randomly assigned into A,B and C groups(n =8).The distal alveolar sockets of group A was immediately implanted with the new composite mineralized collagen membrane and bone graft material,those of group B with bone graft material,group C was the blank control.The healing of sockets was evaluated by gross observation,morphological measurements,X-ray microscope and photographs of spiral CT.Results:The horizontal width of the alveolar process of group A was bigger than that of group B (P ＜ 0.05),that of group B was bigger than that of group C (P ＜ 0.05).The region of extraction interest in spiral CT value was higher in group A than that in group B and C (P ＜ 0.05).The extraction sockets were generated new bone and the degree of reconstruction measurements was higher in group A than that in group B and C (P ＜ 0.05).Conclusion:The new composite mineralized collagen membrane can induce the regeneration of new bone,and preserve the alveolar.

Objective To study on the effectiveness and reliability of endoscopic varicose vein ligation (EVL) combined with hand-assisted laparoscopic splenectomy (HLS) in portal hypertensive patients. Methods Retrospectively 15 cases of EVL combined with HLS were reviewed from June 2001 to October 2004. These patients with severe esophageal varicose and hypersplenism were adapted to perform endoscopic varicose vein ligation, then 1 -2 weeks after the varicose became milder or disappeared, hand-assisted laparoscopic splenectomy was conducted. Results The number of preoperative ligation was 2. 2 times in average. Esophageal varicose was completely disappeared in 11 and mild in 4. There were no postoperative complications, conversion and death in Hand-assisted laparoscopic splenectomy. The average postoperative count of platelets were (38-67) ? 106/L preoperatively raised to (89-310) ? 106/L postoperatively. In the follow up period (average 17. 6 months) , no varicose vein bleeding happened. Conclusions EVL combined with HLS is not only minimal invasive, but also a secure and effective measure for portal hypertension.

Objective To observe the safety and efficacy of the posterior spinal instrumentation (PSI) with preserving of the intraspinal rib head in neurologically intact patients with scoliosis secondary to Neurofibromatosis type 1 (NF1).Methods The clinical and radiographic data of nine NF1 scoliosis patients with rib head protrusion into the spinal canal,who had undergone PSI from August 1998 to March 2012,were retrospectively investigated.The average age of these patients (4 males,5 females) was 15.4±7.6 years,and their neurological status all were Frankel grade E preoperatively.The intraspinal rib head was not resected in all patients intraoperatively.The following parameters,including the magnitude of rib head penetration into the spinal canal (MRPC),the angle between the bilateral rib (ABR),the angle between the dislocated rib and the posterior vertebral wall (ARV),the coronal Cobb angle and the sagittal Cobb angle,were measured before and after surgery.Results The follow-up period was 0.5 to 4.8 years.The average MRPC decreased from preoperative 32.8％±9.9％ to postoperative 16.8％±15.2％ (P=0.026); the average ARV increased from preoperative 34.7°±16.4° to postoperative 47.8°±17.5° (P=0.001); the average ABR decreased from preoperative 83.0°±19.5° to postoperative 67.9°±13.3° (P=0.009); the average coronal Cobb angle decreased from preoperative 70.7° ±17.7° to 35.4°±17.0° immediately after operation (P=0.000) and the sagittal Cobb angle decreased from preoperative 59.7°±17.6° to 24.7°±10.8° immediately after operation (P=0.001).The coronal Cobb angle and sagittal Cobb angle had no significant change during follow-up period.The neurological status was Frankel grade E in all patients immediately after operation and at final follow-up.Conclusion For NF1 scoliosis patients with rib head penetration into the spinal canal without impingement of the spinal cord and neurological deficits,the deformity can be corrected safely and effectively without resecting the intraspinal rib head.