1.Construction of predictive model of depression with secondary mild cognitive impairment in elderly patients and analysis of its applicability
Baoqin YANG ; Fei WANG ; Zheng YU ; Shujun WANG ; Xiaofen ZHOU ; Shanqiong JIANG ; Chengying ZHENG ; Dongsheng ZHOU
Chinese Journal of Geriatrics 2018;37(7):732-737
Objective To build a predictive model of depression with secondary mild cognitive impairment (MCI) in elderly patients based on current clinical diagnosis and treatment technology,and to analyze its application.Methods Elderly patients with depression hospitalized in three hospitals were consecutively included in our study from September 2013 to December 2015 for collecting relevant clinical data,and followed up for 18 months to confirm a prognosis.The follow-up results were used to predict influencing indices for secondary MCI risk,and to verify judgement effectiveness of the critical value of the relevant indices on the window of time of the secondary MCI.Results A total of 216 elderly patients with depression were included in this study,of whom 9 patients were lost to follow-up.Finally,27 patients had secondary MCI,and 180 patients had normal cognitive function during the follow-up period.Cox multiple regression analysis showed that the risk model of secondary MCI in elderly patients with depression was composed of age (HR:1.30,95 % CI:1.12-1.64,P =0.03),education years (HR:0.56,95 % CI:0.41-0.80,P =0.01),regular psychological treatment (HR:0.73,95% CI:0.58-0.92,P=0.03),and BSSI scale (HR:1.24,95% CI:1.08-1.56,P=0.03).Age and BSSI scale were risk factors,while education years and regular group psychotherapy were protective factors.For an elderly patient with depression who was characterized by age ≥ 72.3 years,education years <8.3 years,and BSSI scale ≥75.1,the window of time for secondary MCI was shorter,and these critical values of the independent factors had significant judgement effectiveness.Conclusions Age,education years,regular psychological treatment,and BSSI scale are independently influencing factors for secondary MCI in elderly patients receiving the treatment for depression.Furthermore,age ≥72.3 years,the education period <8.3 years,and BSSI scale ≥75.1 points are critical values of secondary MCI.
2.Clinical application of modified radical neck dissection by gasless unilateral axillary approach in papillary thyroid cancer
Jiajie XU ; Chuanming ZHENG ; Yining ZHANG ; Lingling DING ; Haiwei GUO ; Zhuo TAN ; Jiafeng WANG ; Liehao JIANG ; Zhiqiang SUN ; Ying XIN ; Wanchen ZHANG ; Chengying SHAO ; Minghua GE
Chinese Journal of Endocrine Surgery 2023;17(1):5-10
Objective:To investigate the effectiveness, safety, and advantages of modified radical neck dissection by gasless unilateral axillary approach (GUA-MRND) in the surgical management of selected patients with papillary thyroid cancer.Methods:We retrospectively analyzed patients with papillary thyroid cancer who underwent GUA-MRND (endoscopic group, n=16) versus unilateral open modified radical neck dissection (MRND) (open group, n=32) during the period from Jan. 2019 to Jun. 2021, including the differences in surgical efficiency, complication rate, and incisional satisfaction.Results:Compared MRND with GUA-MRND, the patients were younger ( P<0.05) , operative time and postoperative drainage anterior ( P<0.01) were slightly inferior in the latter, but it had obvious advantages in cervical swallowing discomfort and incision satisfaction evaluation ( P<0.05) . There was no significant difference in the incidence of temporary recurrent laryngeal nerve injury, intraoperative and postoperative bleeding, hematoma, infection, lymphatic or chylous leakage and supraclavicular numbness after surgery ( P>0.05) . The number of dissected lymph nodes in area II in the GUA-MRND was lower ( P<0.05) , but it was significantly higher ( P<0.01) in area III. And the average regional cleaning efficiency in the GUA-MRND was level Ⅲ (35.5%) , level Ⅵ (28.59%) , level Ⅳ (23.21%) , level Ⅱ (7.18%) and level Ⅴ (7.12%) , suggested that GUA-MRND had higher efficacy for level III, level Ⅵ and Level IV. Conclusion:GUA-MRND is safe, effective, and has high cosmetic satisfaction in the treatment of selected patients with lateral cervical lymph node metastases from papillary thyroid cancer.
3.Characteristics of the rhizosphere and phyllosphere microbial community of three submerged plants in the dragon-shaped water system of Beijing.
Xiaodong LIU ; Haizhen ZHU ; Minzhi JIANG ; Xuejun WU ; Chengying JIANG ; Shuang-Jiang LIU
Chinese Journal of Biotechnology 2021;37(10):3663-3674
Aquatic plants and the epiphytic microorganisms are important contributors to the purification of constructed wetlands. Taking the dragon-shaped water system of Beijing Olympic Park as a model, this study analyzed the structure and function of the microbial communities reside the sediment, the water body and the rhizosphere and phyllosphere of three submerged plants-Vallisneria natans, Myriophyllum verticillatum, and Potamogeton pectinatus using high-throughput sequencing technology. The results showed that the microbial diversity from the highest to the lowest were samples from sediment, plant rhizosphere, plant phyllosphere and water. The microbial diversity of plant phyllosphere samples were significantly higher than those of the water body. LEfSe analysis showed that different habitats enriched different microbial groups. The sediments mainly enriched anaerobic microbes, while the water body and the phyllosphere of plants mainly enriched aerobic microbes, and the rhizosphere of plants had the both. Functional prediction analysis showed that the abundance of denitrification marker genes in phyllosphere samples was higher than that in samples from rhizosphere, sediment and water body, and the abundance of denitrification marker genes in phyllosphere samples of M. verticillatum and P. pectinatus was higher than that of V. natans. This study could serve as a guidance for the selection of submerged plants and functional microorganisms for constructed wetlands.
Beijing
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Hydrocharitaceae
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Microbiota
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Rhizosphere
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Water
4.METTL3⁃mediated m6A methylation regulates lipopolysaccharide⁃induced endothelial cell permeability changes
Jianfeng Wang ; Huilin Yu ; Youxin Yu ; Junhui Song ; Chengying Meng ; Wei Jiang ; Delin Hu
Acta Universitatis Medicinalis Anhui 2024;59(6):1023-1028
Objective :
To explore the molecular mechanism of N6⁃methyladenosine ( m6A) methylation mediated by methyltransferase 3 ( METTL3 ) in regulating lipopolysaccharide ( LPS) Ⅳinduced endothelial cell permeability changes.
Methods :
Human umbilical vein endothelial cells ( HUVECs) were cultured in vitro. HUVECs were treated with LPS 50 , 125 , 250 , 500 , 1 000 , 2 000 ng/ml for 24 h. METTL3 mRNA expression was detected by Real⁃time PCR. After HUVECs were intervened with 500 ng/ml for 24 h , the methylation level of m6A was detected , and cell permeability was measured by cell permeability test. Real⁃time PCR and Western blot were used to detect mRNA and protein expression of intercellular junction proteins ( Claudin⁃5 , Occludin and VE⁃caherin) . METTL3 overexpressed stable cell lines were constructed to measure the changes of m6A methylation level and permeability of endothelial cells during METTL3 overexpression.
Results :
Compared to the control group , LPS inhibited the expression of HUVECs METTL3 mRNA , decreased the methylation of m6A , increased the cell permeability , and decreased the mRNA and protein expression of intercellular junction proteins ( Claudin⁃5 , Occludin and VE⁃Caherin) . When METTL3 was overexpressed , the m6A methylation levels of endothelial cells were enhanced , and the increase of endothelial cell permeability induced by LPS was reversed.
Conclusion
METTL3 ⁃mediated m6A methylation can improve the permeability of endothelial cells induced by sepsis.
5. Regulation of hypoxia inducible factor-1α on permeability of vascular endothelial cells and the mechanism
Delin HU ; Youxin YU ; Rong LIANG ; Shunying ZHOU ; Shengliang DUAN ; Zhiyong JIANG ; Chengying MENG ; Wei JIANG ; Huan WANG ; Yexiang SUN ; Linsen FANG
Chinese Journal of Burns 2019;35(3):209-217
Objective:
To investigate the regulation of hypoxia-inducible factor-1α (HIF-1α) on permeability of rat vascular endothelial cells and the mechanism.
Methods:
Twelve male Sprague-Dawley rats aged 35 to 38 days were collected and vascular endothelial cells were separated and cultured. The morphology of cells was observed after 4 days of culture, and the following experiments were performed on the 2nd or 3rd passage of cells. (1) Rat vascular endothelial cells were collected and divided into blank control group, negative control group, HIF-1α interference sequence 1 group, HIF-1α interference sequence 2 group, and HIF-1α interference sequence 3 group according to the random number table (the same grouping method below), with 3 wells in each group. Cells in negative control group, HIF-1α interference sequence 1 group, HIF-1α interference sequence 2 group, and HIF-1α interference sequence 3 group were transfected with GV248 empty plasmid, recombinant plasmid respectively containing HIF-1α interference sequence 1, interference sequence 2, and interference sequence 3 with liposome 2000. Cells in blank control group were only transfected with liposome 2000. After transfection of 24 h, expression levels of HIF-1α mRNA and protein of cells in each group were respectively detected by reverse transcription real-time fluorescent quantitative polymerase chain reaction and Western blotting (the same detecting methods below) . The sequence with the highest interference efficiency was selected. (2) Another batch of rat vascular endothelial cells were collected and divided into blank control group, negative control group, and HIF-1α low expression group, with 3 wells in each group. Cells in blank control group were only transfected with liposome 2000, and cells in negative control group and HIF-1α low expression group were respectively transfected with GV248 empty plasmid and low expression HIF-1α recombinant plasmid selected in experiment (1) with liposome 2000. After 14 days of culture, the mRNA and protein expressions of HIF-1α in each group were detected. (3) Another batch of rat vascular endothelial cells were collected and divided into blank control group, negative control group, and HIF-1α high expression group, with 3 wells in each group. Cells in blank control group were transfected with liposome 2000, and cells in negative control group and HIF-1α high expression group were respectively transfected with GV230 empty plasmid and HIF-1α high expression recombinant plasmid with liposome 2000. After 14 days of culture, the mRNA and protein expressions of HIF-1α of cells in each group were detected. (4) After transfection of 24 h, cells of three groups in experiment (1) and three groups in experiment (2) were collected, and mRNA and protein expressions of myosin light chain kinase (MLCK), phosphorylated myosin light chain (p-MLC), and zonula occludens 1 (ZO-1) of cells were detected. Data were processed with one-way analysis of variance and
6.Microorganisms in the typical anaerobic digestion system of organic solid wastes: a review.
Xingsheng YANG ; Shang WANG ; Qing HE ; Zhujun WANG ; Zhaojing ZHANG ; Chengying JIANG ; Liping MA ; Xianwei LIU ; Baolan HU ; Yongmei LI ; Ye DENG
Chinese Journal of Biotechnology 2021;37(10):3425-3438
The facultative anaerobic and strict anaerobic microorganisms enriched and acclimated during the anaerobic digestion process are crucial for the efficiency of the anaerobic digestion system. Most of the problems encountered during running anaerobic digestion processes could be effectively improved via stimulation of microbial metabolic activity. Benefited from the rapid development of microbiome techniques, deeper insights into the microbial diversity in anaerobic digestion systems, e.g. the microbe-microbe interactions and microbe-environment interactions, have been gained. A complex and intricate metabolic network exists in the anaerobic digestion system of solid organic wastes. However, little is known about these interactions and the underlying mechanisms. This review briefly summarized the representative interactions between microbial communities during anaerobic digestion process discovered to date. In addition, typical issues encountered during the anaerobic digestion of solid organic wastes and how microbes can tackle and alleviate these issues were discussed. Finally, future priorities on microbiome research were proposed based on present contribution of microbiome analysis in anaerobic digestion system.
Anaerobiosis
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Bioreactors
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Methane
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Microbial Interactions
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Microbiota
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Solid Waste