1.Diagnostic utility of detection of CEA mRNA, CEA and CA19-9 in benign and malignant hydrothorax
China Oncology 1998;0(01):-
Purpose:To determine the diagnostic value of detection of carcinoembryonic antigen mRNA (CEA mRNA), CEA and CA19-9 for diagnosis and differential diagnosis of malignant or benign hydrothorax. Methods:The groups included 76 cases of malignant hydrothorax group and 58 cases in benign hydrothorax group from whom specimens of chest fluid were obtined. CEA mRNA was detected by reverse transcriptase-nested primer-polymerase chain reaction (RT-NP-PCR). CEA and CA19-9 were detected by magnetic antibody immunoenzymetric assay (MAIA). Results:The positive rates of CEA mRNA, CEA and CA19-9 were 78.9%(60/76), 52.6%(40/76) and 55.3%(42/76) in the malignant hydrothorax group, and 8.6% (5/58), 5.2%(3/58) and 3.4%(2/58) in the benign dropsy of chest group respectively. There were significant differences in the positive results of CEA mRNA, CEA and CA19-9 between benign and malignant hydrothorax(P
2.An Analysis on Detection of Multipathogens Infection in the Women with Unhealthy Pregnancy
Chengyan XUE ; Hongru SONG ; Haiyang BAI
Journal of Chinese Physician 2001;0(05):-
Chlamydia trachomatic(CT 2 8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B 19 V,CMV,RV,HSV,TOX were significant difference(? 2 test,P=0 000~P=0 031) among the three groups,hovever others were not(? 2 test,P=0 121~P=0 724).Conclusions The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B 19 V,CMV,TOX,RV,HSV.These pathogens infection should be detected timely in gestational period.
3.The Study on the Detection of Pathogens for the Acute Respiratory Infection in the Early Diagnosis of Clinical Etiology
Chengyan XUE ; Xia LIU ; Licheng LIU
Journal of Chinese Physician 2000;0(11):-
Objective To study the practical methods for the diagnosis of clinical etiology of acute respiratory infection(ARI).Methods 108 patients with ARI were the expermental group and forty healthy peoples were the control group.The specimens from throaty excretions were collected in the both groups.Adenovirus(ADV),coxsackie virus(COX),respirator syncytial virus(RSV),chlamidia pneumonia(CP) and mycoplasma pneumonia(MP) in the specimens were detected with polymerase chain reaction(PCR).Results The detected positive rates were 10 2% for ADV,17 6% for COX,28 7% for RSV,33 3% for CP and 13 9% for MP respectively in ARI.There was significantly difference between the ARI group and the healthy control group(P
4.An Analysis on Detection of Multipathogens Infection in the Women with Unhealthy Pregnancy
Chengyan XUE ; Hongru SONG ; Haiyang BAI
Journal of Chinese Physician 2001;3(5):341-343,346
Objective To analysis the relationship between the multipathogens infection and unhealthy pregnancy.Methods All patients was selectively divided into three groups:group A including 106 patients with unhealthy pregnancy,group B:55 gravidas with normal induced abortion,group C:55 normal parturient with term labor.The ten kinds of pathogens from abortion tissues and placentas were tested by PCR method respectively.Anti-pathogen IgM antibodies were detected by ELISA in the blood specimens.Results The distribution status of the pathogens were as followed:Human parvovirus B19(B19V 23.6%)>Cytomegalovirus(CMV,16.0%)>Toxoplasma (TOX,15.1%)>Rubella virus(RV,10.4%)>Ureaplasma urealyticum(9.4%)>Coxsackie(7.5%)>Herpes simplex virus(HSV,6.6%)>Adenovirus,Eprstan Barr virus(3.8%)>Chlamydia trachomatic(CT 2.8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B19V,CMV,RV,HSV,TOX were significant difference(χ2 test,P=0.000~P=0.031) among the three groups,hovever others were not(χ2 test,P=0.121~P=0.724).Conclusions The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B19V,CMV,TOX,RV,HSV.These pathogens infection should be detected timely in gestational period.
5.An estimate on three markers in the monitoring of blood metastasis of lung cancer
Dongmei LU ; Zhiqiang DI ; Chengyan XUE ; Xinsheng DU ; Linying YANG
Cancer Research and Clinic 2010;22(1):41-43
Objective To understand the clinical value on the detection of carcinoembryonic antigen mRNA (CEA mRNA), cytokeratin 19 mRNA (CK19 mRNA) and telomerase in blood for the monitoring of blood metastasis of lung cancer. Methods CEA mRNA and CK19 mRNA were detected by reverse transcriptase-nested primers-polymerase chain reaction, telomerase was detected by telomeric repeat amplification protocol-hybridism-enzyme-linked immunosorbent assay in peripheral blood. Results The positive rates of the three tumor markers in lung cancer group were much higher than the non-tumor group and the health group (P<0.001). The sensibility of CEA mRNA and telomerase were much higher than CK19 mRNA (P<0.01). The positive rates of the markers in TNM stages Ⅲ and Ⅳ were much higher than in stages Ⅰ (P<0.05 to P<0.01). Conclusion It had high value that detecting CEA mRNA, CK19 mRNA and telomerase in peripheral blood on the discovery of blood metastasis of lung cancer. Among them, the clinical value of CEA mRNA and telomerase are higher than CK19 mRNA, and combined assay of the markers can improve the sensibility.
6.A study on the expression of carcinoembryonic antigen mRNA in tissues specimens by reverse transcription polymerase chain reaction in patients with digestive tract carcinoma
Chengyan XUE ; Yan LIANG ; Baoxin SUN ; Al ET
China Oncology 2001;0(03):-
Purpose:To evaluate the differences in expression of carcinoembryonic antigen mRNA (CEA mRNA) in various tissues and its use in the diagnosis of digestive tract carcinoma.Methods:There were 35 patients with digestive tract carcinoma and 12 patients with non carcinoma disease in the study. The expression of CEA mRNA in the digestive tract tissues specimens was detected with nested reverse transcriptase polymerase chain reaction(RT PCR). A contrast test was detection of carcinoembryonic antigen (CEA) in blood sepcimen with enzyme linked immunosorbent assay (ELISA). Results:The expression of CEA mRNA in cancer tissues with RT PCR was 29 (82.86%) with positive results and that of CEA in blood with ELISA was 15 (42.86%) in the patients with digestive tract carcinoma. The difference in positive results was significant ( P
7.Guideline for the diagnosis and treatment of chronic refractory wounds in orthopedic trauma patients (version 2023)
Yuan XIONG ; Bobin MI ; Chenchen YAN ; Hui LI ; Wu ZHOU ; Yun SUN ; Tian XIA ; Faqi CAO ; Zhiyong HOU ; Tengbo YU ; Aixi YU ; Meng ZHAO ; Zhao XIE ; Jinmin ZHAO ; Xinbao WU ; Xieyuan JIANG ; Bin YU ; Dianying ZHANG ; Dankai WU ; Guangyao LIU ; Guodong LIU ; Qikai HUA ; Mengfei LIU ; Yiqiang HU ; Peng CHENG ; Hang XUE ; Li LU ; Xiangyu CHU ; Liangcong HU ; Lang CHEN ; Kangkang ZHA ; Chuanlu LIN ; Chengyan YU ; Ranyang TAO ; Ze LIN ; Xudong XIE ; Yanjiu HAN ; Xiaodong GUO ; Zhewei YE ; Qisheng ZHOU ; Yong LIU ; Junwen WANG ; Ping XIA ; Biao CHE ; Bing HU ; Chengjian HE ; Guanglin WANG ; Dongliang WANG ; Fengfei LIN ; Jiangdong NI ; Aiguo WANG ; Dehao FU ; Shiwu DONG ; Lin CHEN ; Xinzhong XU ; Jiacan SU ; Peifu TANG ; Baoguo JIANG ; Yingze ZHANG ; Xiaobing FU ; Guohui LIU
Chinese Journal of Trauma 2023;39(6):481-493
Chronic refractory wound (CRW) is one of the most challengeable issues in clinic due to complex pathogenesis, long course of disease and poor prognosis. Experts need to conduct systematic summary for the diagnosis and treatment of CRW due to complex pathogenesis and poor prognosis, and standard guidelines for the diagnosis and treatment of CRW should be created. The Guideline forthe diagnosis and treatment of chronic refractory wounds in orthopedic trauma patients ( version 2023) was created by the expert group organized by the Chinese Association of Orthopedic Surgeons, Chinese Orthopedic Association, Chinese Society of Traumatology, and Trauma Orthopedics and Multiple Traumatology Group of Emergency Resuscitation Committee of Chinese Medical Doctor Association after the clinical problems were chosen based on demand-driven principles and principles of evidence-based medicine. The guideline systematically elaborated CRW from aspects of the epidemiology, diagnosis, treatment, postoperative management, complication prevention and comorbidity management, and rehabilitation and health education, and 9 recommendations were finally proposed to provide a reliable clinical reference for the diagnosis and treatment of CRW.