Abstract: Our previous work revealed berberine can significantly enhance the susceptibility of fluconazole against fluconazole-resistant Candida albicans, which suggested that berberine has synergistic antifungal activity with fluconazole. Preliminary SAR of berberine needs to be studied for the possibility of investigating its target and SAR, improving its drug-likeness, and exploring new scaffold. In this work, 13-substitutited benzyl berberine derivatives and N-benzyl isoquinoline analogues were synthesized and characterized by 1H NMR and MS. Their synergetic activity with fluconazole against fluconazole-resistant Candida albicans was evaluated in vitro. The 13-substitutited benzyl berberine derivatives 1a-1e exhibited comparable activity to berberine, which suggested that the introduction of functional groups to C-13 can maintain its activity. The N-benzyl isoquinolines, which were designed as analogues of berberine with its D ring opened, exhibited lower activity than berberine. However, compound 2b, 2c, and 4b showed moderate activity, which indicated that berberine may be deconstructed to new scaffold with synergistic antifungal activity with fluconazole. The results of our research may be helpful to the SAR studies on its other biological activities.

Objective:To explore the latent categories of college students′ regulation emotional self-efficacy and its relationship with social anxiety, so as to provide theoretical basis for different groups to implement relevant intervention.Methods:A total of 415 college students were investigated by scale of regulation emotional self-efficacy(SRESE)and interaction anxiousness scale(SIAS). SPSS 26.0 was used for descriptive statistics and data collation, and Mplus 8.3 was used for latent profile analysis (LPA) to explore the potential categories of regulation emotional self-efficacy of college students. The modified BCH method was used to explore the relationship between different categories of regulation emotional self-efficacy and social anxiety.Results:Regulation emotional self-efficacy can be divided into three categories: " high positive expression and low management negative regulation emotional efficiency" , " low regulation emotional efficiency" and " high regulation emotional efficiency" , accounting for 30.3%, 22.3% and 47.4% of all college students. The three categories had different predictive effects on social anxiety. The " high positive expression and low management negative regulation emotional efficiency" (48.66±0.75) and " the low regulation emotional efficacy" (48.05±0.97) had higher scores in social anxiety and there was no significant difference in the prediction of social anxiety between them( χ2=0.24, P=0.62). However, " high regulation emotional efficiency" ( 45.29±0.56) had a lower score on social anxiety, which was significantly different in the prediction of social anxiety compare the other two categories( χ2=6.06, 12.30, both P<0.05). Conclusion:There are three different potential categories of regulation emotional self-efficacy. Different potential categories of regulation emotional self-efficacy have different social anxiety, so targeted intervention methods can be developed to improve the regulation emotional self-efficacy and reduce social anxiety.