OBJECTIVETo detect the expression of cell cycle positive regulators cyclin D(1), cyclin E, CDK(2), CDK(4) and negative regulators p21(cip1), p27(kip1), p16(ink4a) and p15(ink4b) during wound healing in rats.

METHODSOpen wounds of full-thickness skin, diameter 1.8 cm, on rat backs were used as the wound model. Wound tissues were harvested on postwounding days 3, 5, 7, 9, 11, 14, 21 and 30. Ki67 expression in granulation tissue was detected by immunohistochemical assay. The patterns of the expression of cyclin D(1), cyclin E, CDK(2), CDK(4) and p21(cip1), p27(kip1), p16(ink4a), p15(ink4b) were detected by Western blot.

RESULTSCell proliferation in granulation tissue took place predominantly within the first week after injury, with the proliferation peak occurring at postwounding day 5. There were no dramatic variations in the expression of cyclin D(1), CDK(2) and CDK(4) during wound healing. Up-regulated cyclin E was maintained from day 3 to 11 after injury, and then was down-regulated. No expression of p16(ink4a) and p15(ink4b) was found. p21(cip1) was expressed only from day 7 to 14, with peak expression observed on day 9. Constitutive p27(kip1) was expressed throughout wound healing with low levels in the proliferating period of day 3 to 5 and with increased levels in the post-mitotic and remodeling stage. The expression of p21(cip1) and p27(kip1) showed an inverse gradient to that of Ki67.

CONCLUSIONp21(cip1) and p27(kip1) play a supervising role in preventing the hyperproliferative tendency in tissue repair.

Animals ; Cell Cycle ; physiology ; Cell Cycle Proteins ; biosynthesis ; physiology ; Cell Division ; physiology ; Cyclin-Dependent Kinase Inhibitor p16 ; biosynthesis ; Cyclin-Dependent Kinase Inhibitor p27 ; Cyclin-Dependent Kinases ; antagonists & inhibitors ; biosynthesis ; Cyclins ; biosynthesis ; Male ; Rats ; Rats, Wistar ; Skin ; cytology ; metabolism ; Tumor Suppressor Proteins ; biosynthesis ; physiology ; Wound Healing

Objective To explore the effect of comprehensive nursing intervention on expectoration in patients with acute cerebral vascular disease combined with acute lung injury (ALI).Methods One hundred and fifteen patients with acute cerebral vascular disease combined with ALI were randomly divided into the intervention group ( n=58 ) and the control group ( n=57 ) according to random number table .The intervention group received the comprehensive nursing intervention including the breathing exercises , sputum induction and chest percussion therapy , and the control group received the conventional sputum nursing according to the acute cerebral vascular disease combined with ALI , and the effect of expectoration was compared in two groups . Results No difference was found in the oxygen saturation before the intervention in two groups (P>0.05). The oxygen saturation is (96.58 ±2.31)% after the expectoration in the intervention group , and was higher than (92.53 ±2.73)% in the control group, and the difference was statistically significant (t=8.594, P<0.01).The PaO2 was (81.05 ±1.23) mmHg after the expectoration in the intervention group , and was higher than (77.18 ±1.37) mmHg in the control group, and the difference was statistically significant (t=15.945, P<0.01).The time of effective expectoration , alleviating the symptoms of asthma hold , the disappearance of sputum, body temperature returned to normal and ICU monitoring were respectively (3.57 ±1.25) h, (5.26 ± 1.53) h, (3.52 ±1.63) d, (5.32 ±1.65) d, (7.52 ±1.67) d in the intervention group, and were less than (5.72 ±1.52) h, (9.57 ±2.05) h, (5.93 ±2.15) d, (7.82 ±1.87) d, (11.65 ±2.31) d in the control group, and the differences were statistically significant ( t =8.291, 12.792, 6.781, 7.605, 11.001, respectively;P <0.01).The total efficiency of the recovery of respiratory function after the effective expectoration in the intervention group was 96.55%, and was higher than 82.46%in the control group, and the difference was statistically significant (χ2 =6.116, P <0.01 ).Conclusions Comprehensive nursing intervention can promote the expectoration , increase gas exchange efficiency , improve hypoxia as soon as possible, alleviate the symptoms of asthma hold , and enhance the effect of treatment in patients with acute cerebral vascular disease combined with ALI .