Fifteen patients with hepatocellular failure from viral hepatitis were treated with general combined therapy and transfusion" of homologous fetal hepatic cells (FLO for 36 times (group A). An additional 15 cases with hepatocellular failure from viral hepatitis were treated only with general combined therapy, as control (group B). Twelve cases of the group A were diagnosed by transcutaneous liver biopsy.The results showed that FLC suspension achieved satisfactory effects and those who received FLC rallied remarkably better than those who did not. The fatality rates of group A and B were 26.7% (4/15) and 60.0% (9/15) respectively. In group A after transfusion of FLC, the effects were mainly shown by the elevation of the prothrombin activity (elevated for 16.5% after 24 h and for 18.2% after 48 h in average), recovery from hepatic coma and improvement in the subjective symptoms and various hepatic function tests. In survivals of group A (11 cases), the serum bilirubin returned to normal more quickly than that of group B (5 cases), being 55.4?24.5 days and 119.7?54.9 days, respectively (P

Objective To observe the clinical efficacy of acupuncture, chiropractic plus functional exercise of lumbar and gluteal muscles in treating postpartum sacroiliac malposition.Method Forty patients with postpartum sacroiliac malposition were randomized into group A, group B, group C and group D, 10 cases in each group. Group A was intervened by acupuncture, chiropractic plus functional exercise of lumbar and gluteal muscles, group B by acupuncture plus chiropractic, group C by acupuncture plus functional exercise of lumbar and gluteal muscles, and group D by chiropractic plus functional exercise of lumbar and gluteal muscles. The four groups were intervened once every other day. The modified Oswestry Disability Index (ODI) score was observed before and after 5 treatment sessions, and the clinical efficacies in the four groups were compared.Result The total effective rates were 100.0% in the four groups, and the inter-group differences were statistically insignificant (P>0.05). The ODI scores in the four groups were significantly changed after treatment and in the 3-month follow-up study compared with those before the treatment (P<0.05). After the treatment, the ODI score in group B was significantly different from that in the other groups (P<0.05). The ODI scores in group A and B in the 3-month follow-up study were significantly different from those after the treatment in the same group (P<0.05). The ODI score in group A was significantly different from that in the other groups in the 3-month follow-up study (P<0.05). The ODI score in group B in the 3-month follow-up study was significantly different from that in group C and D (P<0.05).Conclusion Acupuncture, chiropractic plus functional exercise of lumbar and gluteal muscles is definitely effective in treating postpartum sacroiliac malposition, acupuncture plus chiropractic can produce a significant short-term efficacy and alleviate the symptoms, and functional exercise of lumbar and gluteal muscle can produce an obvious long-term efficacy.

A comparison of effectiveness of chemo-immunotherapy (CIT) using LAK/CD3AK 1-2 weeks after TACE in 74 patients with no surgical indication and TACE alone in 41 patients with moderately advanced HCC was carried out. The rates of remission between CIT group and TACE alone group were 72.4% and 40.4%(P

Objective To study the clinical effect of endoscopic therapy in the patients with severe acute pancreatitis(SAP).Methods Totally 289 patients with SAP were divided into endoscopic therapy and control groups in random.The patients in control group received conventional medications.The patients in endoscopic group not only received conventional medications but also took endoscopic sphincterotomy (EST),removing stones,endoscopic nasobile or nasopancreatic drainage within 72 h after onset.The remission time of abdominal pain and distention,the duration of serum amylase back to normal,APACHEⅡscores,hospitalization days and expenditure were compared between two groups.Results The remission time [(10.5?3.0) day] of abdominal pain and distention in endoscopic group was significantly lower than that [(12.4?6.8) day] of control group,P

Objective To evaluate the features of solid-pseudopapiHary tumors of pancreas (SPTP),and to compare with the clinical and pathological findings in order to improve the diagnostic accura-cy.Methods The CT appearances were analyzed retrospectively in 5 patients with pathologically proven SPTP.There were 4 women and 1 man,with the mean age of(16.20±7.49)years.Plain CT and contrasted CT were performed in all 5 cases.Results The tumors of 4 caseswerelocatedinthe head and neck of pan-creats and 1 case was in the body and tail of pancreas.Clear tumor margins were seen in all cases.The mean diameter of these tumors was 5.2 cm(3.0-9.0 cm).Two cases were predominantly composed of cystic por-tions,2 cases were mainly composed of solid portions,and 1 case was composed of similar proportions of solid and cystic portions.Inhomogeneous enhancement Was rewealed in contrasted CT scan,but Was lower in both phase than normal pancreas.Tumor border discontinuous ring-shape calcification was seen in 1 case,dilata-tion of the main pancreatic duct Was demonstrated in 1 case and no metastasis were seen.Conclusions Certain CT features may suggest the diagnosis of SPTP.CT combined with the clinical feature is helpful to the diagnosis.

Objective To investigate the effect of percutaneous transforaminal endoscopic surgery for lumbar prolapse upwards type disc herniation.Methods According to the digital table,90 patients with lumbar prolapse upwards type disc herniation were randomly divide into the observation group and the control group,45 cases in each group.The observation group was treated with percutaneous transforaminal endoscopic surgery,the control group was treated with conventional open surgery.The curative effect,operation and adverse reactions were compared between the two groups.Results All patients were satisfied with the location,and they were completely removed from the nucleus.Compared with the control group,the operation time[(75.3±23.1)min vs.(103.4±35.4)min]and hospitalization time[(6.8±1.2)d vs.(8.7±1.8)d]of the observation group were shorter,the bleeding volume[(24.5±8.5)mL vs.(51.6±12.7)mL]was lower,the differences were statistically significant(t=4.453,5.632,4.269,all P<0.05).After one month and three months,the VAS score[(2.53±0.65)pints and(1.33±0.48)points]and ODI[(21.40±5.04)% and(12.93±3.50)%]of the observation group were significantly lower,there were statistically differences between the two groups compared with those before treatment(t=6.755,8.765,all P<0.05).And after one month,the VAS score and ODI of the observation group were significantly lower than those of the control group,the differences were statistically significant(t=4.342,3.985,all P<0.05).There was no significant difference between the two groups in the excellent and good rate(86.7% vs.82.2%,X2=1.011,P>0.05).ConclusionPercutaneous endoscopic discectomy surgery in the treatment of lumbar prolapse upwards type disc herniation is reliable,and it has less trauma and shorter hospital stay.

Objective To evaluate the efficiency of different interventional mode in treating primary hepatocellular carcinoma with decompensated liver cirrhosis.Methods 132 cases with hepatocellular carcinoma accompanied by decompensational liver cirrhosis were undertaken transcatheter arterial chemo-embolization(TACE,Group A,n=36),segmental transcatheter arterial chemo-embolization(S-TACE,Group B,n=54)and S-TACE + radiofrequency ablation(RFA) + percutaneous ethanol injection(PEI)(multi-interventional therapy,Group C,n=42).Univariate analysis and multivariate COX proportional hazard regression model were used to analyze the factors affecting the prognosis.Results All patients of A,B,C groups had been followed up for 3 months to 48 months,with median survival times of 4.1 months,9.4 months(P 50%) demonstrated as 8.3%,31.5%(P

Because the medical treatment mode is being transformed into 'patients as orientation',our hospital introduces queuing system,transformsand redesigns programs of registration system,outpatient doctor workstation system,outpatient charge system and outpatient dosage system.By using liquid crystal display and automatic phonetic call,the outpatient procedure is optimized with a friendly service environment so as to improve the work efficiency and enhance the overall administration and service quality.

Objective:To construct I-Ad/IgG2b Fc baculovirus expression vector and express I-Ad/IgG2b Fc dimer fusion protein in Sf9 insect cells.Methods:I-Ad α,I-Ad β and IgG2b Fc gene sequences were amplified from BALB/c mouse lymphocytes by RT-PCR.I-Ad α and I-Ad β were connected with the leucine zipper sequence Fos and Jun respectively by overlapping PCR to form I-Ad α-Fos and I-Ad β-Jun.I-Ad α-Fos and IgG2b Fc fragments were ligated by restriction sites Xba I to form I-Ad α-Fos-IgG2b Fc recombination sequence.I-Ad α-Fos-IgG2b Fc and I-Ad β-Jun fragments were inserted to PPH and PP10,which were the downstream of the promoters in the plasmid pFastBacTMDual,to form pFastBacTMDual+[I-Ad/IgG2b Fc] recombinant plasmids.The constructed vector was identified by PCR,restriction endonuclease and sequencing.The recombinant plasmids pFastBacTMDual+[I-Ad/IgG2b Fc] was transferred into the DH10Bac competent cell to form recombinant baculovirus Bacmid+[I-Ad/IgG2b Fc].The recombinant baculovirus was transfected into Sf9 insect cells by liposome transfection reagent.After infected with Sf9 insect cells,the supernatant was collected and concentrated by PEG20000 to obtain I-Ad/IgG2b Fc dimer fusion protein.The fusion protein was detected by double-antibody sandwich ELISA and Western blot.Results:PCR,restriction enzyme digestion and sequencing confirmed that the recombinant vector pFastBacTMDual+[I-Ad/IgG2b Fc] had the correct sequence.The double antibody sandwich ELISA and Western blot showed that recombinant bacmid could successfully infect Sf9 insect cells,and the expressed fusion protein had the correct conformation.Conclusion:The pFastBacTMDual+[I-Ad/IgG2b Fc] baculovirus expression vector was successfully constructed and expressed in Sf9 insect cells,laying a foundation for the study of I-Ad-restricted T cells.

Objective Estimating clinical effects of the Therapy of Potassium L-aspartate Injection on Hypokalemia.Methods These hypolalemia patients from the Renji hospital of Shanghai Jiaotong University and Other Two Hospitals,and from March 2007 to May 2008,which were caused from various kinds of reasons.The test group (42 patients) were intravenous dripped by using three rami potassium L-aspartate injection added in 5% Glucose (or saline) 750 mL,the control group (44 patients) were gived two rami chloratum Kalium injection added in 5% Glucose (or saline) 750 mL,the Kalium were 1.10 g/d in both groups,once per day,time of therapy was 7 day.Results The test and control groups effective power after therapy were 86.67% and 91.11%,respectively,there was no significant difference (P=0.3998).The Kalium dose of test group was advanced from (3.18?0.23) mmol/L before testing to (3.73?0.37) mmol/L after therapy,the elevated average was (0.61?0.46)mmol/L,and there was no significant difference (P=0.4722).The accumulation power of Kalium recovery were (51.11?7.45)%,(77.78?6.20)%,(86.67?5.07)%,at the second,the fored and the sevened day,respectively.There was no significant difference between the two groups (P=0.1430).Conclusion The clinical effect was correspond between three rami potassium L-aspartate injection added in 5% Glucose (or saline) 750 ml and the Isodose chloratum Kalium injection,by once per day,and seven days of therapy,which can treat hypokalimeia caused from various kinds of reasons.