Objective:To establish an HS-GC method for the determination of menthol in Xiao ’ er Fufang Mahuangjian Jiegeng syrups. Methods:An Agilent DB-WAX column(30 m × 0. 53 mm,1. 0 μm)with an FID detector was used;the inlet temperature was 150℃, the detector temperature was 250℃;the flow rate of the carrier gas ( N2 ) was 2. 0 ml·min-1 , the split ratio was 1∶1; the headspace equilibrium temperature was 80℃, the balance time was 30 min, and the sample volume was 1ml. Results:The calibration curve of menthol was within the range of 2. 09-20. 92 μg·ml-1, which showed a good linear relationship (r=0. 999 1). The average recovery of menthol was 98. 0%(RSD=1. 8%, n=6). Conclusion: The method is simple, accurate and reproducible, and can be used for the determination of menthol in Xiao’ er Fufang Mahuangjian Jiegeng syrups.

Objective:To systematically examine the efficacy of mindfulness-based stress reduction (MBSR) for post-traumatic stress disorder (PTSD).Methods:A systematic literature search was performed in multiple databases,including Cochrane Library,Pubmed,MEDLINE,Embase,Web of Science,CBM,CNKI,WanFang Database.Paper inclusion criteria were confined to intervention trials of MBSR conducted in patients with PTSD.Revman5.3 and Stata12.0 was used to get pooled results.Results:A total of 8 studies were included,4 randomized controlled trials (including 255 patients with PTSD) and 4 self-controlled studies (including 152 patients with PTSD).Comparing to control groups,MBSR groups resulted in statistically significant improvements on symp toms of PTSD [SMD =-0.53,95 ％ CI(-0.87--0.19),P ＜ 0.05].The symptoms of PTSD after the intervention was improved [SMD =-1.95,95％ CI(-2.84--1.07),P ＜0.01].There was no statistically significant difference between the data of follow-up and post-intervention [SMD =-0.26,95％ CI(-0.77-0.26),P ＞0.05].Contusion:It suggests that the mindfulness-based stress reduction (MBSR) may be effective in reducing symptoms of post-traumatic stress disorder and have stable short-term effects.

Mitochondrial dynamics, the processes of mitochondrial fusion and fission maintain homeostasis, are precisely regulated by fusion/fission-related proteins, and play an important physiological role in mitochondrial metabolism, quality and function. The aberrant changes of these proteins can trigger mitochondrial dynamics imbalance, which cause mitochondrial dysfunctions and result various disease states. This article focuses on gene knockout technology, and reviews the role and application progress of genes encoding for fusion and fission knockout mice in insulin resistance researches, in order to lay a foundation for future studies on signal transduction mechanism of mitochondrial dynamics imbalance in insulin resistance.
Animals ; Gene Knockout Techniques ; Insulin Resistance ; Mice ; Mitochondria ; Mitochondrial Dynamics ; Mitochondrial Proteins